Table of Content

30 April 2013, Volume 28 Issue 4

Previous Issue    Next Issue

Detection and analysis of the multi-drug resistant regulator A in the clinical isolates

LI Li 1，JIANG Yanqun 2, SHEN Jianxiong 1，XUE Zhizhong 1

2013, 28(4):  259-262.  DOI: 10.3969/j.issn.1673-8640.2013.04.001

Asbtract ( 631 )   HTML   PDF (788KB) ( 578 )  

References | Related Articles | Metrics

Objective　To detect the expression of multi-drug resistant regulator A (MarA) in Escherichia coli，and to analyze the relationship of MarA with the resistance to 3rd and 4th generation cephalosporin.   Methods　A total of 49 isolates of Escherichia coli between 2003 and 2009 were collected randomly. Real-time quantitation reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression levels of anti-putative outer membrane porin F protein (OmpF) antisense RNA-F (MicF)，efflux pump AB (AcrAB) and MarA. The antibiotic sensitivity was also analyzed by disk diffusion method. Results　Among the 49 isolates of Escherichia coli，7 of 49 isolates (14.29%) had high AcrAB transcription level，13 of 49 isolates (26.53%) had MicF transcription level，11 of 49 isolates (22.45%) had high MarA transcription level，and 2 isolates had the 3 resistant factors simultaneously with increasing level. The resistance rates to cefotaxime，ceftazidime and cefepime were 79.59%, 75.51% and 51.02%. The increasing expression level of AcrAB group showed that the resistance rates to these 3 antibiotics were 100.00%，100.00% and 57.14%，respectively. The increasing expression level of MarA group showed that the resistance rates to these 3 antibiotics were 75.00%，100.00% and 58.33%，respectively. The resistance rate increased to 100.00%，when all the 3 resistant factors existed simultaneously. Conclusions　MarA plays an important role in regulating the expressions of AcrAB and MicF. The increasing expression levels of AcrAB and MicF regulated by MarA will lead to high resistance rate to antibiotics.

Significance on the bacterial distribution in blood culture and the positive alarm time

GUAN Youhua，ZHOU Jinfeng，OU Yunzhi

2013, 28(4):  263-266.  DOI: 10.3969/j.issn.1673-8640.2013.04.002

Asbtract ( 594 )   HTML   PDF (647KB) ( 595 )  

References | Related Articles | Metrics

Objective　To analyze the significance on the bacterial distribution in blood culture and the positive alarm time in differentiating the pathogens and contaminated bacteria.   Methods　The retrospective analysis of 642 isolates for blood culture from July 2009 to December 2011 was performed to observe the bacterial distribution, and 148 possible contaminated bacteria were comprehensively analyzed with the clinical data. Results　Of the 642 positive isolates, 338(52.6%)were Gram-negative,280 (43.6%) were Gram-positive, and 24 (3.7%)were fungi. The 376 (58.6%) isolates were detected in<18 h,120 (18.7%) isolates were detected in 19-24 h,89 (13.9%) isolates were detected in 25-48 h, and 57 (8.8%) isolates were detected in＞48 h. Totally 111 of 148 isolates were identified to be contamination by reviewing the clinical data, and the contamination rate was 17.3%.All the contaminated bacteria were Gram-positive, and coagulase negative Staphylococcus was major. The 33 isolates were pathogens, and 4 isolates were indeterminable. The counts of contamination durning 19-24 h, 25-48 h and ＞48 h were 14 isolates, 40 isolates and 57 isolates with positive alarm time ＞ 18 h, respectively. Conclusions　The bacterial distribution in blood culture is wide in this area, and the contamination rate is high. The pathogens and contaminated bacteria can initially be differentiated by the clinical data and other auxiliary examination combining with the positive alarm time according to certain evaluation standard.

Determination and analysis of high-sensitivity cardiac troponin-T in neonates with pathological jaundice

ZOU Hanliang 1，YAN Zhen 2，JIANG Ming 2，HOU Jiaxing 2

2013, 28(4):  267-270.  DOI: 10.3969/j.issn.1673-8640.2013.04.003

Asbtract ( 610 )   HTML   PDF (752KB) ( 949 )  

References | Related Articles | Metrics

Objective　To investigate primarily the differences of serum high-sensitivity cardiac troponin-T （hs-cTnT） in healthy children in different periods and the changes of hs-cTnT in neonates with pathological jaundice，and compare it with creatine kinase MB （CK-MB） and serum total bilirubin （TBil）. Methods　The reference range of serum hs-cTnT in children was determined from 225 healthy children in age from 1 d to 6 years. The levels of hs-cTnT，CK-MB，TBil and direct bilirubin (DBil) were determined and compared between 46 neonates with pathological jaundice and 30 control neonates. The hs-cTnT levels were also compared with those of 31 neonates with preterm delivery，43 neonates with hypoxia and 12 neonates with infections. Results　The reference interval of hs-cTnT was 0-0.074 μg/L. The level of serum hs-cTnT in healthy children decreased with the increasing of age and reached the adult′s level at 6 months after birth. The average level of hs-cTnT in 46 neonates with pathological jaundice was（0.120±0.095）μg/L，and was significantly higher than those in the control group[（0.035±0.020）μg/L]，preterm delivery group [（0.065±0.035） μg/L]，infection group [（0.037±0.017） μg/L] and hypoxia group [（0.051±0.026） μg/L]（P＜0.001）. The levels of TBil, DBil and CK-MB in neonates with pathological jaundice were also significantly higher than those in the control group (P＜0.000 1 and P＜0.05). There was no significant correlation between hs-cTnT and TBil in neonates with pathological jaundice （r=0.088 6，P＞0.05）. Conclusions　In healthy children，the level of serum hs-cTnT decreases with the increasing of age and reaches the adult′s level at 6 months after birth. The level of serum hs-cTnT can be used to indicate the myocardial injury in neonates with pathological jaundice and provide the reference for the early diagnosis，laboratory examination and following therapy in neonates with pathological jaundice.

Research on the early diagnosis and risk stratification significance of the determination for serum H-FABP and MPO in elderly patients with NSTE ACS

CHEN Jian

2013, 28(4):  271-275.  DOI: 10.3969/j.issn.1673-8640.2013.04.004

Asbtract ( 558 )   HTML   PDF (662KB) ( 519 )  

References | Related Articles | Metrics

Objective　To investigate the early diagnosis and risk stratification significance of the determination for serum heart-type fatty acid-binding protein (H-FABP) and myeloperoxidase (MPO) in elderly patients with non-ST-segment elevation acute coronary syndrome (NSTE ACS).   Methods　The 107 patients with NSTE ACS were classified into 2 groups according to the severity of patient′s condition，unstable angina pectoris (UAP) group (38 cases) and non-ST-segment elevation myocardial infarction (NSTEMI) group (69 cases). According to the coronary narrow degree, they were classified into mild narrow group (49 cases)，moderate narrow group (24 cases) and severe narrow group (34 cases). The 107 patients were classified into 3 groups: single-vessel disease group (37 cases)，double-vessel disease group (30 cases) and multi-vessel disease group (40 cases) according to the coronary lesion involving range. The 107 patients were classified into 3 groups： low risk group (51 cases)，average risk group (40 cases) and high risk group (16 cases) according to the global registry of acute coronary event (GRACE) risk score. The levels of serum H-FABP and cardiac troponin I (cTnI) in the 107 patients with NSTE ACS and 31 healthy controls were detected by bidirectional lateral flow immunoassay, and the levels of serum MPO were detected by enzyme-linked immunosorbent assay. The high sensitive C reaction protein (hs-CRP) was determined by latex enhanced immuno-trubidimetry. Results　The levels of serum H-FABP，MPO, cTnI and hs-CRP in NSTEMI group were significantly higher than those in UAP group and the healthy control group (P＜ 0.05), and the levels in UAP group were significantly higher than those in the healthy control group (P＜0.05). In the NSTE ACS group, the levels of serum H-FABP and MPO had close relations with the coronary narrow degree，the coronary lesion involving range and GRACE risk score. The higher the levels of serum H-FABP, MPO and hs-CRP in the NSTE ACS group were，the higher the coronary narrow degree，the coronary lesion involving range and GRACE risk score were. The serum H-FABP, MPO and hs-CRP levels had statistical significance between the groups (P＜0.01), and the levels of H-FABP, MPO and hs-CRP in the coronary narrow degree, the coronary lesion involving range and GRACE risk score groups were higher than those in the healthy control group (P＜0.01). There was a positive correlation of H-FABP with MPO and hs-CRP in the NSTE ACS patients (r=0.555 0 and 0.526 0，P＜0.01). Conclusions　The H-FABP and MPO levels are the biomarkers in NSTE ACS patients. The levels of serum H-FABP and MPO in the NSTE ACS patients have close relations with the coronary narrow degree，the coronary lesion involving range and GRACE risk score. The H-FABP and MPO have the early diagnosis and risk stratification significance in elderly patients with NSTE ACS.

Investigation on the clinical significance of serum anti-acrosin antibodies and anti-sperm protein 17 antibodies in infertility

ZHONG Zhimin，WANG Wei，MO Yundan，DAI Hui

2013, 28(4):  276-279.  DOI: 10.3969/j.issn.1673-8640.2013.04.005

Asbtract ( 629 )   HTML   PDF (855KB) ( 540 )  

References | Related Articles | Metrics

Objective　To investigate the influence and significance of serum anti-acrosin antibodies (AcrAb) and anti-sperm protein 17 antibodies(Sp17Ab) in infertility.   Methods　The levels of serum AcrAb and Sp17Ab were detected by enzyme-linked immunosorbent assay (ELISA) in 60 patients with unexplained infertility and 48 healthy subjects(control group). The results were analyzed by t test and straight line related analysis.   Results　The levels of serum AcrAb in males and females were (22.39±3.15) and (21.02±3.56）U/L in the unexplained infertility group, which were significantly higher than those in the control group [(11.29±2.15) and （10.23±2.12）U/L，P＜0.01]. The levels of serum Sp17Ab in males and females were （10.47±2.10） and （9.63±2.04）U/L in the unexplained infertility group, which were significantly higher than those in the control group [3.63±1.05 and （3.58±1.03）U/L，P＜0.01]. There was no correlation of serum AcrAb with Sp17Ab in males and females [coefficient of correlation (r) = 0.17 and -0.20, P＞0.05]. Conclusions　The levels of serum AcrAb and Sp17Ab in patients with infertility are obviously higher than those in the healthy subjects，and they are independent and can be alone or jointly detected as the indicators of infertility auxiliary diagnosis.

The Level Changes and Clinical Significance of serum EPOin Patients with Acute Coronary Syndrome

NING Li，WEI Dianjun，ZHANG Jingwei

2013, 28(4):  280-282.  DOI: 10.3969/j.issn.1673-8640.2013.04.006

Asbtract ( 270 )   HTML   PDF (641KB) ( 491 )  

References | Related Articles | Metrics

Objective　To study the level and its clinical significance of peripheral blood erythropoietin（EPO） in patients with acute coronary syndrome （ACS）.   Methods　The serum EPO levels were measured by enzyme-linked immunosorbent assay in 68 patients with ACS [35 patients with acute myocardial infarction （AMI） and 33 patients with unstable angina pectoris（UAP）]，28 patients with stable angina pectoris（SAP） and 30 controls with normal coronary arteriography. The ACS patients were classified into intracoronary stent implantation group (26 cases) and un-implantation group (42 cases). Results　The serum EPO level in ACS group [(1.21±0.27)mg/L] was significantly higher than that in SAP group [(0.90±0.23)mg/L] and that in control group [(0.81±0.36)mg/L](P＜0.01). The serum EPO level in AMI group was higher than those in UAP group and control group（P＜0.05）. The serum EPO level in implantation group was higher than that in un-implantation group（P＜0.05）. Conclusions　The serum EPO level is related with ACS. The serum EPO level will increase in ACS patients with intracoronary stent implantation.

The relationship of HBV DNA load and platelet parameters in 878 HBsAg positive patients

ZENG Tingting，JIANG Hong，LUO Shunda，DING Bin，SU Jun

2013, 28(4):  283-286.  DOI: 10.3969/j.issn.1673-8640.2013.04.007

Asbtract ( 525 )   HTML   PDF (649KB) ( 562 )  

References | Related Articles | Metrics

Objective　To investigate the relationship between platelet (PLT) parameters and age，sex，hepatitis B virus (HBV) DNA load and other factors.   Methods　A total of 878 hepatitis B surface antigen (HBsAg) positive patients were enrolled for the determinations of PLT count，thrombocytocrit(PCT)，mean platelet volume (MPV)，platelet distribution width (PDW)，HBV DNA and alanine aminotransferase(ALT)/ aspartate aminotransferase (AST). Variance analysis，regression analysis，one-way ANOVA and 2-sample t-test were used to analyze the data statistically. Results　Factorial analysis demonstrated age，sex and HBV DNA would influence PLT parameters，but ALT/AST would not. PLT and PCT were lower in HBsAg positive patients than in healthy subjects with same age and sex，while MPV and PDW were higher. There was a negative relationship of PLT and PCT with HBV DNA in elderly HBsAg positive patients，while MPV and PDW had no correlation. The 4 PLT parameters in young and middle-aged subjects had no correlation with HBV DNA. Conclusions　PLT and PCT decrease with the increase of HBV DNA load in HBsAg positive patients over 50 years old，which may be related to the suppression or destruction of megakaryocyte differentiation and growth with the increase of HBV DNA load. Monitoring the PLT parameters is of benefit to monitor the hemostatic and coagulative conditions in the patients.

Pathogenic analysis of recurrent vaginitis

YUE Wenyan 1，SHAN Jiangjing 2

2013, 28(4):  287-289.  DOI: 10.3969/j.issn.1673-8640.2013.04.008

Asbtract ( 410 )   HTML   PDF (652KB) ( 560 )  

References | Related Articles | Metrics

Objective　To investigate the species and distribution of pathogens in the vaginal secretion of patients with recurrent vaginitis，and to provide reference for clinical application.  Methods　The vaginal secretion samples were collected from the outpatients with vaginitis in the Department of Gynecology. The normal saline smear method was used to detect the trichomonas，rapid Gram′s staining was used to detect Candida pelliculosa，and sialidase method was used to detect bacterial vaginosis (BV). Results　In 67 patients with recurrent vaginitis，32 cases with pathogen infection were detected(47.76%)，and 19 cases with single pathogen infection were detected (28.36%)，including 10 cases of single Candida pelliculosa infection (14.93%)，1 case of single trichomonas infection (1.49%) and 8 cases of single positive BV (11.94%). A total of 13 cases of mixed infection were detected (19.40%)，among which Candida pelliculosa mixing with positive BV had 10 cases (14.93%)，trichomonas mixing with positive BV had 2 cases (2.99%), and Candida pelliculosa and trichomonas mixing with positive BV had 1 case (1.49%). In 123 cases of control group，50 cases were found with pathogen infection (40.65%), including 40 single infection cases(32.52%) and 10 mixed infection cases(8.13%). Among the 40 single infection cases，single Candida pelliculosa infection was found in 18 cases(14.63%)，single trichomonas infection was found in 2 cases (1.63%)，and single positive BV was found in 20 cases(16.26%). Among the 10 mixed infection cases，Candida pelliculosa mixing with positive BV had 8 cases (6.50%)，and trichomonas mixing with positive BV had 2 cases (1.63%). Conclusions　The pathogen mixed infection in patients with recurrent vaginitis is notable，accounting for nearly half of the detected pathogens，especially the cases mixing with BV，and it should arouse the notice in clinical application.

Evaluation on the clinical application of Sysmex UF-1000i urine formed element analyzer for screening funguria

DING Zhixiang，JIANG Yinfen，ZHOU Yilan，JIN Wentao，QIAN Gaochao

2013, 28(4):  290-292.  DOI: 10.3969/j.issn.1673-8640.2013.04.009

Asbtract ( 629 )   HTML   PDF (692KB) ( 621 )  

References | Related Articles | Metrics

Objective　To evaluate the clinical application significance of Sysmex UF-1000i urine formed element analyzer (UF-1000i analyzer) for screening funguria.   Methods　The precision，linear range and carryover rate of Candida albicans were detected by UF-1000i analyzer. A total of 200 urine samples which UF-1000i analyzer showed like yeast cell（YLC） positive and 400 YLC negative urine samples were detected by manual microscopy. Results　For UF-1000i analyzer detecting Candida albicans，the precision was 3.83%- 4.71%，the linear range was 10.0-5 873.1 cells/μL，the correlation coefficient was 0.999, and the carryover rate was 0.12%. Manual microscopy was as the gold standard，and the result of receiver operating characteristic (ROC) curve showed the best cut-off value for UF-1000i analyzer detecting YLC was 18.05 cells/μL. The sensitivity，specificity，accuracy，positive predictive value and negative predictive value of UF-1000i analyzer in the diagnosis of funguria were 97.0%，85.6%，87.5%，57.4% and 99.3%，respectively. Conclusions　UF-1000i analyzer can provide quantitative results of fungal spores. It aids to diagnose urinary tract fungal infection.

Comparison on the results of Sysmex UF-1000i and AVE764B analyzers and manual microscopy

YING Jun，GUO Wei，MA Jirong，YING Chunmei

2013, 28(4):  293-295.  DOI: 10.3969/j.issn.1673-8640.2013.04.010

Asbtract ( 646 )   HTML   PDF (651KB) ( 601 )  

References | Related Articles | Metrics

Objective　To use the traditional manual microscopy as the standard method，to compare the results of quantitative analysis of Sysmex UF-1000i urine formed element analyzer (UF-1000i analyzer) and AVE764B urinary sediment analyzer (AVE764B analyzer) and to analyze the 2 detection systems′strengths and weaknesses.   Methods　A total of 200 fresh (within 1 h) urine samples of patients from Department of Urinary Surgery and Department of Urology were analyzed，and the 2 systems were evaluated. Results　Manual microscopy and UF-1000i analyzer comparison showed that red blood cell: Y＝1.173X－3.277，r＝0.975，F＝3 771.028，P＝0.000; white blood cell: Y＝0.658X＋35.842，r＝0.973，F＝4 759.543，P＝0.000; epithelial cell: Y＝0.604X＋11.252，r＝0.973，F＝1 378.888，P＝0.000. Manual microscopy and AVE764B analyzer comparison showed that red blood cell: Y＝1.27X－41.857，r＝0.973，F＝3 585.945，P＝0.000; white blood cell: Y＝0.741X－9.402，r＝0.990，F＝10 210.826，P＝0.000; epithelial cell: Y＝0.486X－9.852，r＝0.959，F＝2 271.304，P＝0.000. Conclusions　The results of the 2 systems and manual microscopy had consistent correlation. Urinary sediment analyzer has good recognition analysis for urine formed elements，but for the actual work or some special specimens, manual microscopy identification is needed.

Establishment and verification of blood cell analysis review criteria in women and children specialized hospital

WANG Yuefang，JIANG Yongmei，ZHANG Ge，DU Zeli，CHEN Lan，YE Lei，CHEN Qi

2013, 28(4):  296-300.  DOI: 10.3969/j.issn.1673-8640.2013.04.011

Asbtract ( 575 )   HTML   PDF (658KB) ( 1237 )  

References | Related Articles | Metrics

Objective　To establish proper review criteria for blood cell analysis in women and children specialized hospital. Methods　Based on the review criteria from the cooperation group of formulation for blood cell analysis by Sysmex XE-2100 hematology analyzer，as the women and children specialized hospital with the own unique disease characteristics and children white blood cell (WBC) physiological characteristics，the review criteria was optimized. The total 1 120 samples were detected by Sysmex XE-2100 hematology analyzer， blood smear determination was as the golden standard，and the new review criteria was evaluated and optimized. Results　The review rate of initial review criteria was 65.98%. According to the women and children specialized hospital characteristics，by adjusting the WBC，platelet (PLT) count and WBC differential percentage for the first time after optimization，the false positive rate was 40.71%. By combining immature granulocyte (IG) and WBC≥10×109/L，combining blast cell (Blast) and WBC≤4×10⁹/L or ≥10×10⁹/L，and combining atypical/variant lymphocyte (Lymph) (Atypical/Variant) and Lymph%＞50% for the second time after optimization，the false positive and false negative rates were 17.53% and 4.49%. The main items were Atypical/Variant Lymph （<5%）and PLT＞70×10⁹/L，which were verified. The proper review criteria in women and children specialized hospital was established. Conclusions　The review criteria for women and children specialized hospital should be established in clinical application，improve the review criteria timely，and improve the analysis quality and efficiency of blood cell analysis.

Detection of peripheral blood high mobility group protein box-1 in patients with gastric cancer and its clinical significance

XIAO Jinhua，ZHU Huayan，WANG Yaping，PAN Yuhong，HUANG Xuan

2013, 28(4):  301-304.  DOI: 10.3969/j.issn.1673-8640.2013.04.012

Asbtract ( 586 )   HTML   PDF (651KB) ( 503 )  

References | Related Articles | Metrics

Objective　To investigate the change and clinical significance of serum high mobility group protein box-1 (HMGB1) in patients with gastric cancer.   Methods　The serum levels of HMGB1 were determined by enzyme-linked immunosorbent assay (ELISA) in 101 patients with gastric cancer，31 patients with benign gastric diseases and 40 healthy controls. Simultaneously，serum carcinoembryonic antigen(CEA) was determined by electrochemiluminescence (ECL). The results were expressed as median (range). Results　The levels of serum HMGB1 and CEA in patients with gastric cancer were 15.58（3.05-125.63）ng/mL and 11.75（0.85-850.23）ng/mL. The levels of serum HMGB1 and CEA in patients with benign gastric diseases were 4.46（2.61-12.06）ng/mL and 2.75（0.52-11.23）ng/mL. The levels of serum HMGB1 and CEA in healthy controls were 3.24（2.50-5.38）ng/mL and 1.23（0.45- 4.56）ng/mL. The levels of serum HMGB1 and CEA in patients with gastric cancer were significantly higher than those in patients with benign gastric diseases and healthy controls ( P＜0.01）. The levels of serum HMGB1 were significantly associated with tumor size，gastric wall invasion, TNM stage and lymph node metastasis（P＜0.05）. Compared to the traditional tumor marker CEA, the diagnostic sensitivity and specificity of serum HMGB1 were 72.2% and 74.6% for the diagnosis of early gastric cancer.  Conclusions　HMGB1 plays a role in the tumorigenesis and development of gastric cancer. The determination of HMGB1 in serum of patients with gastric cancer is helpful for improving early diagnosis, monitoring and predicting the prognosis in gastric cancer.

Establishment of TRFIA for anti-thyroglobulin antibody and its clinical application

ZHU Lan，HUANG Biao，ZHANG Jue，CHEN Yongwei，LI Yuesong

2013, 28(4):  305-307.  DOI: 10.3969/j.issn.1673-8640.2013.04.013

Asbtract ( 508 )   HTML   PDF (652KB) ( 536 )  

References | Related Articles | Metrics

Objective　To establish a time-resolved fluoroimmunoassay（TRFIA）for anti-thyroglobulin antibody（TgAb）.   Methods　The thyroglobulin antigen was coated on the 96 well plates. A goat anti-mouse IgG-europium (Eu^3＋) was used to the detection. Serum TgAb level was detected by TRFIA.  Results　The sensitivity was 1.0 IU/mL. The within-run coefficient of variation (CV)was 3.3%- 4.1%. The between-run CV was 3.7%- 4.7%. The average recovery was 97.9%. The kit was stable. The correlation coefficient with TgAb by electrochemiluminescence immunoassay (ECLIA) was 0.881 3. The TgAb levels between males and females had no statistical significance. The normal range of TgAb was ≤110 IU/mL.  Conclusions　The established TRFIA for the detection of TgAb in serum is a sensitive and reliable assay, which would be useful for the clinical diagnosis of thyroid diseases.

Research on correlation between immunophenotype and c-MYC rearrangement in diffuse large B-cell lymphoma

WANG Zhilin 1，YAO Yuhua 2，LI Sang 1，SUN Guanxing 1

2013, 28(4):  308-311.  DOI: 10.3969/j.issn.1673-8640.2013.04.014

Asbtract ( 468 )   HTML   PDF (919KB) ( 685 )  

References | Related Articles | Metrics

Objective　To investigate the correlation between immunophenotype and c-MYC rearrangement in diffuse large B-cell lymphoma (DLBCL).  Methods　According to the lymphoma cell immune marker CD10，B cell lymphoma 6 (BCL-6) and multiple myeloma oncogene 1 (MUM-1) expression levels，46 cases of DLBCL were classified into germinal center B cell-like(GCB) group and non-germinal center B cell-like(non-GCB) group. c-MYC rearrangement status was analyzed by fluorescence in situ hybridization (FISH) in GCB and non-GCB groups. Results　Of the 46 DLBCL cases，4 cases (8.7%) had c-MYC rearrangement. The 4 cases of c-MYC rearrangement belonged to the GCB group. There was no c-MYC rearrangement in non-GCB group. The c-MYC rearrangement had statistical significance between GCB and non-GCB groups (P＜0.05). Conclusions　The GCB group in patients with DLBCL has a high c-MYC rearrangement，and c-MYC rearrangement is a poor predictor for a part of GCB group. FISH is a rapid，accurate and sensitive technique for the detection of c-MYC rearrangement.

The investigation of the retesting range of the TPAb sample detected by ELISA

YU Hong，ZHU Wei，CUI Yangyang，QUE Jinya

2013, 28(4):  312-314.  DOI: 10.3969/j.issn.1673-8640.2013.04.015

Asbtract ( 589 )   HTML   PDF (642KB) ( 548 )  

References | Related Articles | Metrics

Objective　To investigate the retesting range of anti-treponema pallidum antibody (TPAb) sample detected by enzyme-linked immunosorbent assay(ELISA).  Methods　A total of 1 257 samples were collected in the first TPAb testing with absorbance (A) value ＞ 0.08 by ELISA. They were retested duplicately by the same reagent kit of same lot number. The samples retested with A vaule ＞ 0.14 were comfirmed by treponema pallidum particale agglutination (TPPA) test.The appropriate retesting range was analyzed statistically. Results　The samples were classified into 7 groups according to the first A value by ELISA: 0.08-0.14，0.15-0.30，0.31-0.60，0.61-0.90，0.91-1.20，1.21-2.00 and ＞2.00. The retesting coincidence rates by ELISA were 16.6%(37/223)，21.6%(56/259)，32.0%(66/206)，50.5%(100/198)，78.0%(99/127)，94.8%(147/155) and 100.0%(89/89). The coincidence rates by TPPA and retesting by ELISA were 0.0%(0/37)，3.6%(2/56)，22.7%(15/66)，38.0%(38/100)，80.8%(80/99)，93.2%(137/147) and 96.6%(86/89). Conclusions　The A value of the retesting range by ELISA is considered appropriate from 0.14-1.20 under a standardized laboratory condition.The appropriate retesting range can assure the accuracy of the testing results and can avoid the waste of manpower and reagents.

The relationship of IFN-γ，IL-10 and CD19＋ levels with viral load in chronic hepatitis B patients

CHEN Yongqin, JIN Wenjun, DAI Menglu

2013, 28(4):  315-317.  DOI: 10.3969/j.issn.1673-8640.2013.04.016

Asbtract ( 616 )   HTML   PDF (644KB) ( 483 )  

References | Related Articles | Metrics

Objective　To observe the change of immune function in patients with chronic hepatitis B, and to investigate the relationship of interferon-gamma(IFN-γ), interleukin 10 (IL-10) and CD19^＋ with hepatitis B virus（HBV） DNA load.   Methods　By enzyme-linked immunosorbent assay (ELISA) and flow cytometry, the serum IFN-γ, IL-10 and peripheral blood CD19^＋ levels of 76 patients with chronic hepatitis B and 30 healthy subjects were determined. By fluorescence quantitative polymerase chain reaction (PCR), serum HBV DNA copy number was determined. Results　The IFN-γ levels in HBV DNA low copy group and high copy group decreased significantly compared with those in HBV DNA negative group. Their IL-10 and CD19^＋ levels increased significantly compared with those in HBV DNA negative group. The IFN-γ levels were correlated negatively with the HBV DNA load (r＝－0.311,P＜0.05)， and the IL-10 and CD19^＋ levels were correlated positively with the HBV DNA load (r＝0.350,P＜0.05; r＝0.321, P＜0.05).  Conclusions　The patients have immune function adjustment disorder. The IFN-γ, IL-10 and CD19^＋ levels can be monitoring indices for the inflammatory of hepatitis B related diseases.

Evaluation on the speciality skill examination of laboratory diagnostic residents from 2004 to 2011

WANG Yin, WANG Qingzhong, ZHOU Jing

2013, 28(4):  318-321.  DOI: 10.3969/j.issn.1673-8640.2013.04.017

Asbtract ( 458 )   HTML   PDF (825KB) ( 452 )  

References | Related Articles | Metrics

Objective　To evaluate the standardized training quality of laboratory diagnostic residents from 2004 to 2011 in Shanghai.  Methods　The scores of 537 examinees, attending Shanghai speciality skill examination of laboratory diagnostic residents from 2004 to 2011, were analyzed. According to the levels of hospitals (Level 3 general hospitals, Level 3 specialized hospitals and Level 2 hospitals), the scores were classified and analyzed. Results　The cycle training rates of Level 3 general hospitals, Level 3 specialized hospitals and Level 2 hospitals were 100.0%, 90.2% and 29.7%. The passing percentages of practice parts(78%) were much better than those of theory parts(63%). The passing percentages of biochemistry and hematology (60% and 67%) were poorer than those of microbiology and immunology(80% and 98%). The scores of examinees were statistically significant between the 3 kinds of hospitals（P＜0.05）. The scores of Level 3 general hospitals were higher than those of the other 2 kinds of hospitals, and the scores of Level 3 specialized hospitals were higher than those of Level 2 hospital. The scores of biochemistry, hematology and microbiology examinees had statistical significance in the 3 kinds of hospitals (P＜0.05), but the scores of immunology examinees had no statistical significance(P＞0.05). Conclusions　Improving the standardized training and examination system is an effective way on improving the speciality skill of laboratory diagnostic residents.