Abstract: Objective　To investigate the influence and significance of serum anti-acrosin antibodies (AcrAb) and anti-sperm protein 17 antibodies(Sp17Ab) in infertility.   Methods　The levels of serum AcrAb and Sp17Ab were detected by enzyme-linked immunosorbent assay (ELISA) in 60 patients with unexplained infertility and 48 healthy subjects(control group). The results were analyzed by t test and straight line related analysis.   Results　The levels of serum AcrAb in males and females were (22.39±3.15) and (21.02±3.56）U/L in the unexplained infertility group, which were significantly higher than those in the control group [(11.29±2.15) and （10.23±2.12）U/L，P＜0.01]. The levels of serum Sp17Ab in males and females were （10.47±2.10） and （9.63±2.04）U/L in the unexplained infertility group, which were significantly higher than those in the control group [3.63±1.05 and （3.58±1.03）U/L，P＜0.01]. There was no correlation of serum AcrAb with Sp17Ab in males and females [coefficient of correlation (r) = 0.17 and -0.20, P＞0.05]. Conclusions　The levels of serum AcrAb and Sp17Ab in patients with infertility are obviously higher than those in the healthy subjects，and they are independent and can be alone or jointly detected as the indicators of infertility auxiliary diagnosis.

Key words: Anti-acrosin antibody, Anti-sperm protein 17 antibody, Infertility