Drug resistance of Ureaplasma isolated from infertile males by microdilution method

doi:10.3969/j.issn.1673-8640.2018.12.004

Abstract

Abstract:

Objective To determine the the drug resistance of Ureaplasma to macrolides,quinolones and tetracyclines in infertile males,to provide a reference for the rational use of antimicrobial agents.Methods Ureaplasma were isolated and purified from semen samples of infertile males by solid culture medium. The minimum inhibitory concentration（MIC） of 12 kinds of antimicrobial agents against 64 isolates of Ureaplasma were determined by microdilution method.Results The MIC of Ureaplasma was 0.015-64.000 μg/mL against the 12 kinds of antimicrobial agents. For Ureaplasma,the MIC₅₀ was 16 μg/mL,and the MIC₉₀ was 64 μg/mL against ciprofloxacin. The MIC₅₀ was 0.25 μg/mL,and the MIC₉₀was 0.5 μg/mL against doxycycline. The drug resistance rates of Ureaplasma to ciprofloxacin,levofloxacin and antofloxacin were 92.2%,70.3% and 64.0%,respectively,according to the Clinical and Laboratory Standards Institute documents and related reference. The drug resistance rates of Ureaplasma to azithromycin,josamycin and doxycycline were 0.0%,1.6% and 3.1%,respectively.Conclusions The MIC of Ureaplasma against the 12 kinds of antimicrobial agents are distributed widely. Ciprofloxacin has low antimicrobial ability,and doxycycline has high antimicrobial ability. Quinolones have high drug resistance to Ureaplasma. Azithromycin,josamycin and doxycycline can be recommended as primary antimicrobial agents for the treatment of Ureaplasma infection in infertile males.

Key words: Microdilution method, Ureaplasma, Male infertility, Drug resistance, Minimum inhibitory concentration

CLC Number:

R446.1

ZHOU Yunheng, SHI Xiaoxing, YANG Yang, GU Weiming, MA Hongxia. Drug resistance of Ureaplasma isolated from infertile males by microdilution method[J]. Laboratory Medicine, 2018, 33(12): 1076-1080.

Figures/Tables 4

References 16

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抗菌药物	S	I	R
左氧氟沙星	≤2	-	≥4
莫西沙星	≤2	-	≥4
四环素	≤1	-	≥2
红霉素	≤8	-	≥16

抗菌药物	S	I	R
左氧氟沙星	≤2	-	≥4
莫西沙星	≤2	-	≥4
四环素	≤1	-	≥2
红霉素	≤8	-	≥16

抗菌药物	测定结果	标准要求
左氧氟沙星	1、2、1	0.5~2
莫西沙星	0.5、0.5、1	0.5~2
四环素	2、0.5、0.5
红霉素	2、2、4	1~8

抗菌药物	测定结果	标准要求
左氧氟沙星	1、2、1	0.5~2
莫西沙星	0.5、0.5、1	0.5~2
四环素	2、0.5、0.5
红霉素	2、2、4	1~8

抗菌药物	S[株（%）]	R[株（%）]	MIC范围（μg/mL）	MIC₅₀（μg/mL）	MIC₉₀（μg/mL）
红霉素	58（90.6）	6（9.4）	0.030~16	4.00	8.00
阿奇霉素	64（100）	0（0）	0.060~8	1.00	2.00
罗红霉素	30（46.9）	24（37.5）	0.060~16	2.00	8.00
克拉霉素	61（93.7）	3（6.3）	0.015~8	0.25	1.00
交沙霉素	63（98.4）	1（1.6）	0.015~8	0.25	1.00
左氧氟沙星	19（29.7）	45（70.3）	0.5~64	8.00	16.00
莫西沙星	51（79.4）	13（20.3）	0.06~16	1.00	4.00
环丙沙星	5（7.8）	59（92.2）	0.5~64	16.00	64.00
安妥沙星	17（26.5）	41（64.0）	1~64	16.00	32.00
四环素	58（90.6）	6（9.4）	0.015~4	0.50	1.00
多西环素	62（96.9）	2（3.1）	0.015~2	0.125	0.50
米诺环素	58（90.6）	3（6.3）	0.015~8	0.50	1.00