Laboratory Medicine ›› 2023, Vol. 38 ›› Issue (5): 435-440.DOI: 10.3969/j.issn.1673-8640.2023.05.006
Previous Articles Next Articles
ZHU Yurong, ZHANG Dan, HE Yaxing, LI Jingjing, HUANG Jing, LI Ting, LIU Peng, LIU Ronghua()
Received:
2022-06-06
Revised:
2022-12-12
Online:
2023-05-30
Published:
2023-08-07
CLC Number:
ZHU Yurong, ZHANG Dan, HE Yaxing, LI Jingjing, HUANG Jing, LI Ting, LIU Peng, LIU Ronghua. Classification and epidemiology characteristics of Klebsiella pneumoniae isolated from clinic[J]. Laboratory Medicine, 2023, 38(5): 435-440.
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.shjyyx.com/EN/10.3969/j.issn.1673-8640.2023.05.006
引物名称 | 引物序列(5'~3') | 退火温度/℃ |
---|---|---|
rpoB | F:GGCGAAATGGCWGAGAACCA | 55 |
R:GAGTCTTCGAAGTTGTAACC | ||
TEM | F:ACAATAACCCTGGTAAATGC | 55 |
R:GAGTAAACTTGGTCTGACAG | ||
CTX | F:TTTCCAATGTGCAGTACCAGTAA | 60 |
R:CAATATCATTGGTGGTGCCGTA | ||
SHV | F:GCCCTCACTCAAGGATGTAT | 55 |
R:TTAGCGTTGCCAGTGCTCGA | ||
ACC(6')- Ib-cr | F:TTGCGATGCTCTATGAGTGGCTA | 62 |
R:CTCGAATGCCTGGCGTGTTT | ||
LEN | F:GCCCTCACTCAAGGATGTAT | 55 |
R:TTAGCGTTGCCAGTGCTCGA | ||
OKP | F:GGCCGGYGAGCGGGGCTCA | 62 |
R:AGAAGCATCCTGCTGTGCG | ||
qnrA | F:TCAGCAAGAGGATTTCTCA | 55 |
R:GGCAGCACTATTACTCCCA | ||
qnrB | F:GATCGTGAAAGCCAGAAAGG | 55 |
R:ACGATGCCTGGTAGTTGTCC | ||
qnrS | F:ACGACATTCGTCAACTGCAA | 60 |
R:TAAATTGGCACCCTGTAGGC | ||
YBT | F:GACGGAAACAGCACGGTAAA | 62 |
R:GAGCATAATAAGGCGAAAGA | ||
ENT | F:GTCAACTGGGCCTTTGAGCCGTC | 60 |
R:TATGGGCGTAAACGCCGGTGAT | ||
KFU | F:GGCCTTTGTCCAGAGCTACG | 62 |
R:GGGTCTGGCGCAGAGTATGC | ||
fimH | F:ATGAACGCCTGGTCCTTTGC | 60 |
R:GCTGAACGCCTATCCCCTGC | ||
mrkD | F:AAGCTATCGCTGTACTTCCGGCA | 60 |
R:GCGTTGGCGCTCAGATAGG | ||
Wzi | F:GTGCCGCGAGCGCTTTCTATCTT-GGTATTCC | 55 |
R:GAGAGCCACTGGTTCCAGAA[C/T]TT[C/G]ACCGC |
引物名称 | 引物序列(5'~3') | 退火温度/℃ |
---|---|---|
rpoB | F:GGCGAAATGGCWGAGAACCA | 55 |
R:GAGTCTTCGAAGTTGTAACC | ||
TEM | F:ACAATAACCCTGGTAAATGC | 55 |
R:GAGTAAACTTGGTCTGACAG | ||
CTX | F:TTTCCAATGTGCAGTACCAGTAA | 60 |
R:CAATATCATTGGTGGTGCCGTA | ||
SHV | F:GCCCTCACTCAAGGATGTAT | 55 |
R:TTAGCGTTGCCAGTGCTCGA | ||
ACC(6')- Ib-cr | F:TTGCGATGCTCTATGAGTGGCTA | 62 |
R:CTCGAATGCCTGGCGTGTTT | ||
LEN | F:GCCCTCACTCAAGGATGTAT | 55 |
R:TTAGCGTTGCCAGTGCTCGA | ||
OKP | F:GGCCGGYGAGCGGGGCTCA | 62 |
R:AGAAGCATCCTGCTGTGCG | ||
qnrA | F:TCAGCAAGAGGATTTCTCA | 55 |
R:GGCAGCACTATTACTCCCA | ||
qnrB | F:GATCGTGAAAGCCAGAAAGG | 55 |
R:ACGATGCCTGGTAGTTGTCC | ||
qnrS | F:ACGACATTCGTCAACTGCAA | 60 |
R:TAAATTGGCACCCTGTAGGC | ||
YBT | F:GACGGAAACAGCACGGTAAA | 62 |
R:GAGCATAATAAGGCGAAAGA | ||
ENT | F:GTCAACTGGGCCTTTGAGCCGTC | 60 |
R:TATGGGCGTAAACGCCGGTGAT | ||
KFU | F:GGCCTTTGTCCAGAGCTACG | 62 |
R:GGGTCTGGCGCAGAGTATGC | ||
fimH | F:ATGAACGCCTGGTCCTTTGC | 60 |
R:GCTGAACGCCTATCCCCTGC | ||
mrkD | F:AAGCTATCGCTGTACTTCCGGCA | 60 |
R:GCGTTGGCGCTCAGATAGG | ||
Wzi | F:GTGCCGCGAGCGCTTTCTATCTT-GGTATTCC | 55 |
R:GAGAGCCACTGGTTCCAGAA[C/T]TT[C/G]ACCGC |
抗菌药物 | KpⅠ (135株) | KpⅡ (4株) | KpⅢ (4株) |
---|---|---|---|
头孢呋辛 | 14(10.37) | 1(25.00) | 0(0) |
头孢唑啉 | 47(34.81) | 3(75.00) | 2(50.00) |
头孢他啶 | 11(8.15) | 0(0) | 0(0) |
头孢哌酮 | 16(11.85) | 0(0) | 0(0) |
头孢哌酮-舒巴坦 | 6(4.44) | 0(0) | 0(0) |
头孢曲松 | 16(11.85) | 0(0) | 0(0) |
头孢吡肟 | 14(10.37) | 0(0) | 0(0) |
头孢西丁 | 16(11.85) | 1(25.00) | 0(0) |
哌拉西林 | 29(21.48) | 2(50.00) | 2(50.00) |
哌拉西林-他唑巴坦 | 7(5.19) | 1(25.00) | 0(0) |
阿莫西林-棒酸 | 13(9.63) | 0(0) | 0(0) |
丁胺卡那霉素 | 4(2.96) | 0(0) | 0(0) |
庆大霉素 | 15(11.11) | 0(0) | 0(0) |
妥布霉素 | 14(10.37) | 0(0) | 0(0) |
环丙沙星 | 20(14.81) | 0(0) | 0(0) |
左氧氟沙星 | 17(12.59) | 0(0) | 0(0) |
美罗培南 | 1(0.74) | 0(0) | 0(0) |
亚胺培南 | 1(0.74) | 0(0) | 0(0) |
米诺环素 | 14(10.37) | 0(0) | 0(0) |
抗菌药物 | KpⅠ (135株) | KpⅡ (4株) | KpⅢ (4株) |
---|---|---|---|
头孢呋辛 | 14(10.37) | 1(25.00) | 0(0) |
头孢唑啉 | 47(34.81) | 3(75.00) | 2(50.00) |
头孢他啶 | 11(8.15) | 0(0) | 0(0) |
头孢哌酮 | 16(11.85) | 0(0) | 0(0) |
头孢哌酮-舒巴坦 | 6(4.44) | 0(0) | 0(0) |
头孢曲松 | 16(11.85) | 0(0) | 0(0) |
头孢吡肟 | 14(10.37) | 0(0) | 0(0) |
头孢西丁 | 16(11.85) | 1(25.00) | 0(0) |
哌拉西林 | 29(21.48) | 2(50.00) | 2(50.00) |
哌拉西林-他唑巴坦 | 7(5.19) | 1(25.00) | 0(0) |
阿莫西林-棒酸 | 13(9.63) | 0(0) | 0(0) |
丁胺卡那霉素 | 4(2.96) | 0(0) | 0(0) |
庆大霉素 | 15(11.11) | 0(0) | 0(0) |
妥布霉素 | 14(10.37) | 0(0) | 0(0) |
环丙沙星 | 20(14.81) | 0(0) | 0(0) |
左氧氟沙星 | 17(12.59) | 0(0) | 0(0) |
美罗培南 | 1(0.74) | 0(0) | 0(0) |
亚胺培南 | 1(0.74) | 0(0) | 0(0) |
米诺环素 | 14(10.37) | 0(0) | 0(0) |
荚膜血清型 | KpⅠ (135株) | KpⅡ (4株) | KpⅢ (4株) |
---|---|---|---|
K1 | 31(22.96) | 3(75.00) | 0(0) |
K2 | 25(18.52) | 0(0) | 0(0) |
K5 | 7(5.19) | 0(0) | 0(0) |
K23 | 5(3.70) | 0(0) | 0(0) |
K30 | 4(2.96) | 0(0) | 0(0) |
K54 | 4(2.96) | 0(0) | 0(0) |
K57 | 17(12.59) | 0(0) | 1(25.00) |
K61 | 0(0) | 0(0) | 2(50.00) |
K126 | 0(0) | 0(0) | 1(25.00) |
其他 | 42(31.11) | 1(25.00) | 0(0) |
荚膜血清型 | KpⅠ (135株) | KpⅡ (4株) | KpⅢ (4株) |
---|---|---|---|
K1 | 31(22.96) | 3(75.00) | 0(0) |
K2 | 25(18.52) | 0(0) | 0(0) |
K5 | 7(5.19) | 0(0) | 0(0) |
K23 | 5(3.70) | 0(0) | 0(0) |
K30 | 4(2.96) | 0(0) | 0(0) |
K54 | 4(2.96) | 0(0) | 0(0) |
K57 | 17(12.59) | 0(0) | 1(25.00) |
K61 | 0(0) | 0(0) | 2(50.00) |
K126 | 0(0) | 0(0) | 1(25.00) |
其他 | 42(31.11) | 1(25.00) | 0(0) |
耐药基因 | KpⅠ (135株) | KpⅡ (4株) | KpⅢ (4株) |
---|---|---|---|
ACC(6')-Ib-cr | 17(12.59) | 0(0) | 0(0) |
qnrA | 1(0.74) | 0(0) | 0(0) |
qnrB | 20(14.81) | 0(0) | 0(0) |
qnrS | 16(11.85) | 0(0) | 0(0) |
LEN | 130(96.30) | 4(100.00) | 4(100.00) |
SHV | 134(99.26) | 4(100.00) | 4(100.00) |
OKP | 131(97.03) | 4(100.00) | 4(100.00) |
CTX | 18(13.33) | 0(0) | 0(0) |
TEM | 13(9.63) | 0(0) | 0(0) |
耐药基因 | KpⅠ (135株) | KpⅡ (4株) | KpⅢ (4株) |
---|---|---|---|
ACC(6')-Ib-cr | 17(12.59) | 0(0) | 0(0) |
qnrA | 1(0.74) | 0(0) | 0(0) |
qnrB | 20(14.81) | 0(0) | 0(0) |
qnrS | 16(11.85) | 0(0) | 0(0) |
LEN | 130(96.30) | 4(100.00) | 4(100.00) |
SHV | 134(99.26) | 4(100.00) | 4(100.00) |
OKP | 131(97.03) | 4(100.00) | 4(100.00) |
CTX | 18(13.33) | 0(0) | 0(0) |
TEM | 13(9.63) | 0(0) | 0(0) |
毒力基因 | KpⅠ(135株) | KpⅡ(4株) | KpⅢ(4株) |
---|---|---|---|
fimH | 133(98.52) | 4(100.00) | 4(100.00) |
mrkD | 132(97.78) | 4(100.00) | 4(100.00) |
ENT | 132(97.78) | 4(100.00) | 4(100.00) |
KFU | 99(73.33) | 4(100.00) | 4(100.00) |
YBT | 74(54.81) | 0(0) | 1(25.00) |
毒力基因 | KpⅠ(135株) | KpⅡ(4株) | KpⅢ(4株) |
---|---|---|---|
fimH | 133(98.52) | 4(100.00) | 4(100.00) |
mrkD | 132(97.78) | 4(100.00) | 4(100.00) |
ENT | 132(97.78) | 4(100.00) | 4(100.00) |
KFU | 99(73.33) | 4(100.00) | 4(100.00) |
YBT | 74(54.81) | 0(0) | 1(25.00) |
[1] |
WANG G, ZHAO G, CHAO X, et al. The characteristic of virulence,biofilm and antibiotic resistance of Klebsiella pneumoniae[J]. Int J Environ Res Public Health, 2020, 17(17):6278.
DOI URL |
[2] | 全国细菌耐药监测网2014—2019年细菌耐药性监测报告[J]. 中国感染控制杂志, 2021, 20(1):15-31. |
[3] |
RODRÍGUEZ-MEDINA N, BARRIOS-CAMACHO H, DURAN-BEDOLLA J, et al. Klebsiella variicola:an emerging pathogen in humans[J]. Emerg Microbes Infect, 2019, 8(1):973-988.
DOI URL |
[4] |
WYRES K L, HOLT K E. Klebsiella pneumoniae as a key trafficker of drug resistance genes from environmental to clinically important bacteria[J]. Curr Opin Microbiol, 2018, 45:131-139.
DOI URL |
[5] |
MAATALLAH M, VADING M, KABIR M H, et al. Klebsiella variicola is a frequent cause of bloodstream infection in the stockholm area,and associated with higher mortality compared to K. pneumoniae[J]. PLoS One, 2014, 9(11):e113539.
DOI URL |
[6] | 尚红, 王毓三, 申子瑜. 全国临床检验操作规程[M]. 4版. 北京: 人民卫生出版社, 2015. |
[7] | WEINSTEIN M P, LEWIS J S 2nd. The clinical and laboratory standards institute subcommittee on antimicrobial susceptibility testing:background,organization,functions,and processes[J]. J Clin Microbiol, 2020, 58(3):e01864-19. |
[8] |
HARADA S, ISHII Y, SAGA T, et al. Molecular epidemiology of Klebsiella pneumoniae K1 and K2 isolates in Japan[J]. Diagn Microbiol Infect Dis, 2018, 91(4):354-359.
DOI URL |
[9] | LONG S W, LINSON S E, OJEDA SAAVEDRA M, et al. Whole-genome sequencing of human clinical Klebsiella pneumoniae isolates reveals misidentification and misunderstandings of Klebsiella pneumoniae,Klebsiella variicola,and Klebsiella quasipneumoniae[J]. mSphere, 2017, 2(4):e00290-17. |
[10] |
GARZA-RAMOS U, BARRIOS-CAMACHO H, MORENO-DOMÍNGUEZ S, et al. Phenotypic and molecular characterization of Klebsiella spp. isolates causing community-acquired infections[J]. New Microbes New Infect, 2018, 23:17-27.
DOI URL |
[11] |
BARRIOS-CAMACHO H, SILVA-SÁNCHEZ J, CERCAS-AYALA E, et al. PCR system for the correct differentiation of the main bacterial species of the Klebsiella pneumoniae complex[J]. Arch Microbiol, 2021, 204(1):73.
DOI |
[12] | 鞠启文, 王运中, 陶云珍, 等. 儿童血流感染肺炎克雷伯菌毒力与耐药基因分析[J]. 中华医院感染学杂志, 2021, 31(21):3337-3341. |
[13] |
ONISHI R, SHIGEMURA K, OSAWA K, et al. Impact on quinolone resistance of plasmid-mediated quinolone resistance gene and mutations in quinolone resistance-determining regions in extended spectrum beta lactamase-producing Klebsiella pneumoniae isolated from urinary tract infection patients[J]. Pathog Dis, 2022, 80(1):ftac030.
DOI URL |
[14] | 吕承秀, 陶欣荣. 肺炎克雷伯菌质粒携带的耐药基因研究进展[J]. 锦州医科大学学报, 2019, 40(6):104-108. |
[15] | JOMEHZADEH N, AHMADI K, BAHMANSHIRI M A. Investigation of plasmid-mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae in southwest Iran[J]. J Clin Lab Anal, 2022, 36(7):e24342. |
[16] | 陈国忠, 俞燕红, 应建飞, 等. ICU产肺炎克雷伯菌喹诺酮类药物耐药相关基因研究[J]. 中国卫生检验杂志, 2021, 31(13):1564-1566. |
[17] |
MARTIN R M, BACHMAN M A. Colonization,infection,and the accessory genome of Klebsiella pneumoniae[J]. Front Cell Infect Microbiol, 2018, 8:4.
DOI URL |
[18] | 施瑜, 王震, 张汉园, 等. 肺炎克雷伯菌荚膜血清型、毒力基因和耐药性研究[J]. 国际检验医学杂志, 2021, 42(14):1720-1725. |
[19] | 吴乾. 某院肺炎克雷伯菌分布及其耐药性与携带毒力基因种数的关系[J]. 检验医学与临床, 2021, 18(19):2900-2902. |
[1] | FANG Yongmei, ZHANG Yan, XU Xueying, ZHONG Feng. Correlation of Staphylococcus aureus drug resistance with drug resistance genes and virulence genes [J]. Laboratory Medicine, 2023, 38(4): 357-361. |
[2] | WANG Jiawei, ZHU Weinan, CHEN Yingying, JI Ping, WANG Ying. Clinical characteristics,molecular typing and drug resistance genes of carbapenem-resistant Klebsiella pneumoniae isolated from blood culturing [J]. Laboratory Medicine, 2023, 38(4): 362-367. |
[3] | DENG Chen, DAI Jiaze, QIU Lihong, SHEN Weiting, CAI Mufa, LUO Wenying. Molecular epidemic characteristics of hypervirulent Klebsiella pneumoniae in Zhanjiang [J]. Laboratory Medicine, 2023, 38(11): 1026-1031. |
[4] | WANG Su, DING Li, JIANG Wenrong, MIAO Yingxin, ZHANG Yanmei, ZHAO Hu. Research progress on drug resistance and treatment strategies of hypervirulent Klebsiella pneumoniae [J]. Laboratory Medicine, 2023, 38(1): 81-86. |
[5] | PAN Yunqi, LI Yungai, WANG Jianqiang, WU Qiong, TANG Jin. Analysis of molecular typing,virulence genes and antibiotic susceptibility of uropathogenic Escherichia coli in patients with recurrent urinary tract infection [J]. Laboratory Medicine, 2022, 37(2): 141-145. |
[6] | KANG Yi, ZHU Hong, SHEN Han, ZHOU Wanqing. Virulence gene analysis of Enterococcus isolated from clinical blood and urine samples [J]. Laboratory Medicine, 2022, 37(11): 1030-1033. |
[7] | ZHANG Guanyi, YAO Dongting, WANG Jian, HU Xiaobo. Research progress on Chinese and western medicine treatment for Candida glabrata infection [J]. Laboratory Medicine, 2021, 36(7): 761-765. |
[8] | HUANG Yun, LI Congrong. Research progress on hypervirulent Klebsiella pneumonia [J]. Laboratory Medicine, 2021, 36(11): 1181-1185. |
[9] | ZHOU Yanxia, JIN Dongfang. Inhibition and mechanism of tannic acid on biofilm formation in Klebsiella pneumoniae [J]. Laboratory Medicine, 2021, 36(10): 1070-1073. |
[10] | LU Shuhua, LI Xiaozhe, LIU Lingyun, JIN Chengqiang, GE Ningning, DONG Haixin. Clinical infection characteristics and their drug resistance genes of carbapenem-resistant Enterobacteriaceae in a hospital of Shandong [J]. Laboratory Medicine, 2020, 35(8): 757-762. |
[11] | DU Jinlong, FENG Yan, LI Hengtao. Role of real-time fluorescence quantitative PCR-binding probe in the determination of MP and MP drug resistance [J]. Laboratory Medicine, 2020, 35(6): 535-539. |
[12] | WANG Junjie, YAO Zonghui, MA Bing, LI Yi, YAN Wenjuan, WANG Shanmei, MA Qiong, YUAN Youhua. Risk factors and prognosis of bloodstream infection caused by carbapenem-resistant Klebsiella pneumoniae [J]. Laboratory Medicine, 2020, 35(3): 195-199. |
[13] | GUO Wei, HUANG Jianghua, WU Bei, CHEN Fang, HE Xiaohua. Drug resistance and virulence distribution characteristics of clinical isolates of Enterococcus in a hospital [J]. Laboratory Medicine, 2020, 35(12): 1267-1271. |
[14] | HE Lihua, NI Lijun, YANG Simin, YU Xiaoyu, ZHOU Aiping, HU Liang, GUO Jian, WU Wenjuan. Clinical application of RT-PCR for Klebsiella pneumoniae producing KPC carbapenemase [J]. Laboratory Medicine, 2020, 35(10): 983-987. |
[15] | WEN Weihong, LIANG Yingjian, MA Furong, LI Yuzhen, XU Lingqing. Carbapenemase detection and multilocus sequence typing analysis of carbapenem-resistant Enterobacteriaceae [J]. Laboratory Medicine, 2019, 34(7): 577-582. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||