Analysis of molecular typing,virulence genes and antibiotic susceptibility of uropathogenic Escherichia coli in patients with recurrent urinary tract infection

doi:10.3969/j.issn.1673-8640.2022.02.009

Abstract

Abstract:

Objective To investigate molecular typing,virulence genes and antibiotic susceptibility of uropathogenic Escherichia coli in patients with recurrent urinary tract infection（rUTI）. Methods Totally,60 patients with rUTI were enrolled,and the isolates of Escherichia coli were isolated. Pulsed-field gel electrophoresis（PFGE）,multi-locus sequence typing（MLST）and phylogenetic groups were performed. Polymerase chain reaction（PCR） and sequencing were used to analyze common Escherichia coli virulence genes（feoB,chuA,iutA,iroN,ireA,fimH,foc,sfa,aer and cnf）. The antibiotic susceptibilities of Escherichia coli to 13 common antimicrobial agents were analyzed. Results The research subjects were classified into relapse and reinfection groups by PFGE. A total of 30 isolates from relapse group and 60 isolates from reinfection group were collected for further analysis. MLST indicated that ST131 was the predominant type followed by ST405. The major phylogenetic groups were B2 and D. Among the 10 virulence genes,feoB（98.8%） was the most common virulence gene followed by fimH（93.3%）. Except for iutA（P=0.020）,no statistical significance of the other virulence genes was found between the 2 groups（P>0.05）. Conclusions The predominant types of uropathogenic Escherichia coli are ST405 and ST131 in Shanghai Sixth People's Hospital. Two virulence genes,feoB and fimH,are highly prevalent. The carry rate of iutA is high in relapse group,which can be as the treatment target for rUTI.

Key words: Uropathogenic Escherichia coli, Recurrent urinary tract infection, Pulsed-field gel electrophoresis, Virulence gene

CLC Number:

R446.1

PAN Yunqi, LI Yungai, WANG Jianqiang, WU Qiong, TANG Jin. Analysis of molecular typing,virulence genes and antibiotic susceptibility of uropathogenic Escherichia coli in patients with recurrent urinary tract infection[J]. Laboratory Medicine, 2022, 37(2): 141-145.

Figures/Tables 4

References 24

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基因或DNA片段	碱基长度/bp	引物序列（5'~ 3'）	退火温度/ ℃
chuA	1 500	F:GCTGTTTCTGCCACCTTG	55
		R:CCTGAGTTTCGTTAGTTTCC
yjaA	211	F:TGAAGTGTCAGGAGACGCTG	55
		R:ATGGAGAATGCGTTCCTCAAC
TSPE4.C2	152	F:GAGTAATGTCGGGGCATTCA	53
		R:CGCGCCAACAAAGTATTACG

基因或DNA片段	碱基长度/bp	引物序列（5'~ 3'）	退火温度/ ℃
chuA	1 500	F:GCTGTTTCTGCCACCTTG	55
		R:CCTGAGTTTCGTTAGTTTCC
yjaA	211	F:TGAAGTGTCAGGAGACGCTG	55
		R:ATGGAGAATGCGTTCCTCAAC
TSPE4.C2	152	F:GAGTAATGTCGGGGCATTCA	53
		R:CGCGCCAACAAAGTATTACG

组别	菌株数/株	A		B1		B2		D		合计
组别	菌株数/株	菌株数/株	百分比/%	菌株数/株	百分比/%	菌株数/株	百分比/%	菌株数/株	百分比/%	菌株数/株	百分比/%
再感染组	60	2	3.3	15	25.0	12	20.0	31	51.7	60	100.0
复发组	30	1	3.3	4	13.3	6	20.0	19	63.3	30	100.0

组别	菌株数/株	A		B1		B2		D		合计
组别	菌株数/株	菌株数/株	百分比/%	菌株数/株	百分比/%	菌株数/株	百分比/%	菌株数/株	百分比/%	菌株数/株	百分比/%
再感染组	60	2	3.3	15	25.0	12	20.0	31	51.7	60	100.0
复发组	30	1	3.3	4	13.3	6	20.0	19	63.3	30	100.0

组别	黏附基因			铁获取基因						毒力基因 cnf
组别	fimH	sfa	foc	foeB	chuA	ireA	iutA	iroN	aer	毒力基因 cnf
再感染组	27（90.0）	1 （3.3）	6 （20.0）	29（96.7）	25（83.3）	3 （10.0）	23（76.7）	19（63.3）	20（66.7）	0 （0）
复发组	57（95.0）	2 （3.3）	7 （11.7）	60（100.0）	44（73.3）	10（16.7）	55（91.7）	36（60.0）	40（66.7）	5 （8.3）
P值	0.396	1.000	0.289	0.333	0.290	0.396	0.020	0.760	1.000	0.165