Abstract:

Objective　To investigate the resistance mechanism and epidemiology of carbapenem-resistant Klebsiella spp， and to establish the pulsed-field gel electrophoresis（PFGE） typing database of carbapenem-resistant Klebsiella spp， in order to provide the reference for rapidly source-tracking and controlling the bacterium spread at the outbreak of nosocomial infection. Methods　Carbapenem-resistant Klebsiella spp.were collected，and the phenotypic and genetic features were detected by modified Hodge test and polymerase chain reaction（PCR）， respectively. PFGE typing was performed for homology analysis. Results　The main resistance mechanism of carbapenem-resistant Klebsiella spp. was Klebsiella pneumoniae carbapenemase（KPC）-producing， and there were 2 genotypes by PFGE typing， including genotype A（4 isolates） and genotype B（4 isolates）. Conclusions　KPC-producing is the main reason for carbapenem-resistant Klebsiella spp. in the hospital， and it is necessary to monitor the resistance and to strengthen the infection control.

Key words: Carbapenem, Modified Hodge test, Polymerase chain reaction, Pulsed-field gel electrophoresis