Laboratory Medicine ›› 2022, Vol. 37 ›› Issue (1): 68-71.DOI: 10.3969/j.issn.1673-8640.2022.01.014
Previous Articles Next Articles
YAO Lifeng, ZHENG Qiaoping, YANG Meng, LUO Qingqiong, CHEN Xu(
), CHEN Fuxiang()
Received:2021-06-10
Revised:2021-08-06
Online:2022-01-30
Published:2022-03-07
Contact:
CHEN Xu,CHEN Fuxiang
CLC Number:
YAO Lifeng, ZHENG Qiaoping, YANG Meng, LUO Qingqiong, CHEN Xu, CHEN Fuxiang. Evaluation of colloidal gold immunochromatography assay for the rapid determination of carbapenemase in CRE isolates[J]. Laboratory Medicine, 2022, 37(1): 68-71.
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.shjyyx.com/EN/10.3969/j.issn.1673-8640.2022.01.014
| 目的基因 | 序列（5'~3'） | 片段大小/bp | |
|---|---|---|---|
| blaKPC | F： | CGTCTAGTTCTGCTGTCTTG | 798 |
| R： | GTTGTCATCCTTGTTAGGCG | ||
| blaNDM | F： | GGTTTGGCGATCTGGTTTTC | 621 |
| R： | CGGAATGGCTCATCACGATC | ||
| blaIMP | F： | GGAATAGAGTGGCTTAATTCTC | 232 |
| R： | GGTTTAATAAAACAACCACC | ||
| blaOXA | F： | GCGTGGTTAAGGATGAACAC | 438 |
| R： | CATCAAGTTCAACCCAACCG | ||
| blaVIM | F： | GATGGTGTTTGGTCGCATA | 390 |
| R： | CGAATGCGCAGCACCAG | ||
| 目的基因 | 序列（5'~3'） | 片段大小/bp | |
|---|---|---|---|
| blaKPC | F： | CGTCTAGTTCTGCTGTCTTG | 798 |
| R： | GTTGTCATCCTTGTTAGGCG | ||
| blaNDM | F： | GGTTTGGCGATCTGGTTTTC | 621 |
| R： | CGGAATGGCTCATCACGATC | ||
| blaIMP | F： | GGAATAGAGTGGCTTAATTCTC | 232 |
| R： | GGTTTAATAAAACAACCACC | ||
| blaOXA | F： | GCGTGGTTAAGGATGAACAC | 438 |
| R： | CATCAAGTTCAACCCAACCG | ||
| blaVIM | F： | GATGGTGTTTGGTCGCATA | 390 |
| R： | CGAATGCGCAGCACCAG | ||
| [1] |
VAN DUIN D, ARIAS C A, KOMAROW L, et al. Molecular and clinical epidemiology of carbapenem-resistant Enterobacterales in the USA(CRACKLE-2):a prospective cohort study[J]. Lancet Infect Dis, 2020, 20(6):731-741.
DOI URL |
| [2] |
DURANTE-MANGONI E, ANDINI R, ZAMPINO R. Management of carbapenem-resistant Enterobacteriaceae infections[J]. Clin Microbiol Infect, 2019, 25(8):943-950.
DOI URL |
| [3] |
KOTB S, LYMAN M, ISMAIL G, et al. Epidemiology of carbapenem-resistant Enterobacteriaceae in Egyptian intensive care units using national healthcare-associated infections surveillance data,2011-2017[J]. Antimicrob Resist Infect Control, 2020, 9(1):2.
DOI URL |
| [4] |
DAVID S, REUTER S, HARRIS S R, et al. Epidemic of carbapenem-resistant Klebsiella pneumoniae in Europe is driven by nosocomial spread[J]. Nat Microbiol, 2019, 4(11):1919-1929.
DOI URL |
| [5] | SUAY-GARCÍA B, PÉREZ-GRACIA M T. Present and future of carbapenem-resistant Enterobacteriaceae(CRE) Infections[J]. Antibiotics(Basel), 2019, 8(3):122. |
| [6] | TAVOSCHI L, FORNI S, PORRETTA A, et al. Prolonged outbreak of New Delhi metallo-beta-lactamase-producing carbapenem-resistant Enterobacterales(NDM-CRE),Tuscany,Italy,2018 to 2019[J]. Euro Surveill, 2020, 25(6):2000085. |
| [7] |
MATSUMURA Y, PEIRANO G, BRADFORD P A, et al. Genomic characterization of IMP and VIM carbapenemase-encoding transferable plasmids of Enterobacteriaceae[J]. J Antimicrob Chemother, 2018, 73(11):3034-3038.
DOI URL |
| [8] | PITOUT J D D, PEIRANO G, KOCK M M, et al. The global ascendency of OXA-48-type carbapenemases[J]. Clin Microbiol Rev, 2019, 33(1):e00102-e00119. |
| [9] |
HAN R, SHI Q, WU S, et al. Dissemination of carbapenemases(KPC,NDM,OXA-48,IMP,and VIM) among carbapenem-resistant Enterobacteriaceae isolated from adult and children patients in China[J]. Front Cell Infect Microbiol, 2020, 10:314.
DOI URL |
| [10] |
TAMMA P D, GOODMAN K E, HARRIS A D, et al. Comparing the outcomes of patients with carbapenemase-producing and non-carbapenemase-producing carbapenem-resistant Enterobacteriaceae bacteremia[J]. Clin Infect Dis, 2017, 64(3):257-264.
DOI URL |
| [11] | 任艳丽, 王云英, 蒋敏, 等. 不同碳青霉烯酶酶型肠杆菌科细菌感染的治疗策略研究[J]. 中国抗生素杂志, 2021, 46(4):339-345. |
| [12] |
NORDMANN P, POIREL L, DORTET L. Rapid detection of carbapenemase-producing Enterobacteriaceae[J]. Emerg Infect Dis, 2012, 18(9):1503-1507.
DOI URL |
| [13] | 胡锡池, 胡仁静, 刘小云. Carba NP直接纸片法检测肠杆菌科细菌碳青霉烯酶的应用评估[J]. 临床与病理杂志, 2017, 37(10):2043-2049. |
| [14] |
YOSHIOKA N, HAGIYA H, DEGUCHI M, et al. Multiplex real-time PCR assay for six major carbapenemase genes[J]. Pathogens, 2021, 10(3):276.
DOI URL |
| [15] |
HUANG T S, LEE S S, LEE C C, et al. Detection of carbapenem-resistant Klebsiella pneumoniae on the basis of matrix-assisted laser desorption ionization time-of-flight mass spectrometry by using supervised machine learning approach[J]. PLoS One, 2020, 15(2):e0228459.
DOI URL |
| [16] | 李东明, 王宇凡, 武玉晶, 等. 耐碳青霉烯革兰阴性杆菌耐药性及基因分型[J]. 中华医院感染学杂志, 2021, 31(6):816-820. |
| [17] |
WOZNIAK A, PAILLAVIL B, LEGARRAGA P, et al. Evaluation of a rapid immunochromatographic test for detection of KPC in clinical isolates of Enterobacteriaceae and Pseudomonas species[J]. Diagn Microbiol Infect Dis, 2019, 95(2):131-133.
DOI URL |
| [18] | Centers for Disease Control and Prevention. Facility guidance for control of carbapenem-resistant Enterobacteriaceae（CRE）[EB/OL]. (2015-11-01)[2021-01-03]. https://www.cdc.gov/hai/organisms/cre/cre-toolkit/. |
| [19] |
POIREL L, WALSH T R, CUVILLIER V, et al. Multiplex PCR for detection of acquired carbapenemase genes[J]. Diagn Microbiol Infect Dis, 2011, 70(1):119-123.
DOI URL |
| [20] |
VARDAKAS K Z, TANSARLI G S, RAFAILIDIS P I, et al. Carbapenems versus alternative antibiotics for the treatment of bacteraemia due to Enterobacteriaceae producing extended-spectrum β-lactamases:a systematic review and meta-analysis[J]. J Antimicrob Chemother, 2012, 67(12):2793-2803.
DOI URL |
| [21] |
XU L, SUN X, MA X. Systematic review and meta-analysis of mortality of patients infected with carbapenem-resistant Klebsiella pneumoniae[J]. Ann Clin Microbiol Antimicrob, 2017, 16(1):18.
DOI URL |
| [22] | 胡付品, 郭燕, 朱德妹, 等. 2018年CHINET中国细菌耐药性监测[J]. 中国感染与化疗杂志, 2020, 20(1):1-10. |
| [1] | SHI Yingying, PAN Fen, SUN Yan, WANG Chun, JIANG Jie, YU Fangyuan, ZHANG Tiandong, QIN Huihong, ZHANG Hong. Nested multiple polymerase chain reaction in rapid diagnosis of meningitis/encephalitis in children [J]. Laboratory Medicine, 2023, 38(8): 766-770. |
| [2] | DU Jinghui, HE Xin, LIU Jing, LIU Ruiyan, BAO Zhijun, WANG Jun, ZHAO Yan, LEI Yaorong, LIU Min, LIU Xu. Evaluation of 7 oscillating mixing methods for SARS-CoV-2 nucleic acid determination [J]. Laboratory Medicine, 2023, 38(7): 680-685. |
| [3] | DAI Fangfang, LU Xinxin, YU Yanhua, CHEN Ming, SUN Guizhen. Analysis of Mycobacterium infection in HIV/AIDS patients [J]. Laboratory Medicine, 2023, 38(12): 1173-1176. |
| [4] | WANG Shiqi, LI Henan, LIU Jingbo, WANG Zhanwei, WANG Zhiren, CHI Dali, WANG Hui. Application evaluation of loop-mediated isothermal amplification in determining Clostridioides difficile in fecal samples [J]. Laboratory Medicine, 2022, 37(9): 860-863. |
| [5] | XU Qi, PAN Fen, SUN Yan, SHI Yingying, YU Fangyuan, ZHANG Hong. Methodology evaluation of screening techniques for CRE colonized in intestinal tract [J]. Laboratory Medicine, 2022, 37(8): 761-765. |
| [6] | WANG Xiaoyu, SEMIRE Ainiwaer, LI Shijun. Influence of specimen state on the accuracy of plasma Epstein-Barr virus DNA determination results [J]. Laboratory Medicine, 2022, 37(7): 615-617. |
| [7] | WAN Changchun, YANG Hui, ZHUANG Xuewei. Application of polymerase chain reaction melting curve method in determining hypertensive drug-related gene mutations [J]. Laboratory Medicine, 2022, 37(7): 646-651. |
| [8] | CHEN Hanlu, WU Shenghai. Influence factors of blood culture negative results and progress of pathogen detection in bloodstream infection [J]. Laboratory Medicine, 2022, 37(7): 688-694. |
| [9] | SUN Lei, LONG Ju, FAN Zuqian, WU Suping, LIN Guixian, LING Xiuming. sRapid determination of sex chromosome abnormality using double relative quantitative SD-qPCR [J]. Laboratory Medicine, 2022, 37(6): 557-560. |
| [10] | SUN Ying, LI Yi, YAN Wenjuan, JING Nan, MA Bing. Analysis of drug resistance of rare CRE and clinical application of carbapenem screening test in Henan [J]. Laboratory Medicine, 2022, 37(2): 146-149. |
| [11] | LIU Wenxia, WANG Wei, LIN Songyang, LI Zhenhong. Inhibitory elimination effects of different enhancers on urea,heme and cholate in fluorescent PCR [J]. Laboratory Medicine, 2022, 37(2): 155-158. |
| [12] | LI Renqiang, LUO Junfeng, CHEN Yundi. Establishment and evaluation of α-thalassemia and β-thalassemia mutation determinations using hetero-tail dual-labeled fluorescent probe hydridization [J]. Laboratory Medicine, 2022, 37(10): 955-962. |
| [13] | LIU Hongqian, SONG Chaohui, LIANG Qiaomi. Establishment and clinical application of multiple RT-PCR MassARRAY for the determinations of 27 respiratory pathogens [J]. Laboratory Medicine, 2021, 36(9): 939-946. |
| [14] | WANG Bowen, ZENG Xiaohua, PENG Kaige, LIU Chang, WU Yingchao, HUANG Yingmei, MA Panpan, Lamuciren , LI Guokai, YANG Li. Influence of different inactivating conditions on nucleic acid determination for respiratory pathogens [J]. Laboratory Medicine, 2021, 36(8): 805-808. |
| [15] | LIU Shiyun, TAN Ping. Etiological analysis of influenza surveillance in Chizhou from 2018 to 2019 [J]. Laboratory Medicine, 2021, 36(7): 722-725. |
| Viewed | ||||||
|
Full text |
|
|||||
|
Abstract |
|
|||||
