Laboratory Medicine ›› 2022, Vol. 37 ›› Issue (9): 860-863.DOI: 10.3969/j.issn.1673-8640.2022.09.013

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Application evaluation of loop-mediated isothermal amplification in determining Clostridioides difficile in fecal samples

WANG Shiqi1, LI Henan1(), LIU Jingbo1, WANG Zhanwei1, WANG Zhiren1, CHI Dali2, WANG Hui1   

  1. 1. Department of Clinical Laboratory,Peking University People's Hospital,Beijing 100044,China
    2. Reference Testing Center,Fosun Diagnostics(Shanghai) Co. Ltd.,Shanghai 200444,China
  • Received:2021-10-16 Revised:2022-06-18 Online:2022-09-30 Published:2022-10-25
  • Contact: LI Henan

Abstract:

Objective To evaluate a loop-mediated isothermal amplification(LAMP) for the rapid determination of Clostridioides difficile in fecal samples from patients. Methods Totally,132 clinical fecal samples were collected and determined by LAMP,bacterium culture and toxin Clostridioides difficile assay,respectively. The consistency of the 3 methods was evaluated. The specificity and sensitivity were evaluated. Results The sensitivity of LAMP for determing Clostridioides difficile reached 105 CFU/mL. The positive rate of LAMP(13.64%) was higher than that of bacterium culture(11.11%,χ2=18.74,P<0.05) and that of toxin Clostridioides difficile assay(9.09%,χ2=35.94,P<0.05). The LAMP showed all negative for the 11 bacteria and 2 fungi determined by bacterium culture. Conclusions Compared with bacterium culture and toxin Clostridioides difficile assay,the LAMP surpasses in good specificity and short reaction time. It can be regarded as a rapid determination method for Clostridioides difficile.

Key words: Clostridioides difficile, Loop-mediated isothermal amplification, Rapid determination

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