Virulence genes and drug resistance of Vibrio parahaemolyticus isolated from patients with diarrhea in Ningbo

doi:10.3969/j.issn.1673-8640.2019.02.009

Abstract

Abstract:

Objective To investigate the virulence genes,type Ⅲ secretion system（T3SS） and drug resistance of Vibrio parahaemolyticus isolated from patients with diarrhea in Ningbo. Methods A total of 49 isolates of Vibrio parahaemolyticus were isolated from feces specimens of patients with diarrhea. The virulence genes,tdh,trh and T3SS1,T3SS2,were determined by polymerase chain reaction（PCR）. The drug susceptibility of 17 kinds of antibiotics were determined by disk diffusion assay. Results Among the 49 isolates of Vibrio parahaemolyticus,48 isolates carried tdh. There was 1 isolate carrying trh,47 isolates showed tdh（+）trh（-）,1 isolate showed tdh（+）trh（+）,and 1 isolate showed tdh（-）trh（-）. The 49 isolates carried T3SS1 gene,47 isolates carried T3SS2α gene,and 1 isolate carried T3SS2β gene. The drug resistance rate to ampicillin was 75.5%. The drug susceptibility rates were high to the 3rd generation cephalosporins and aminoglycoside tetracyclines. All the isolates were sensitive to cefepimes,levofloxacins and carbapenems（imipenem and meropenem）,and the drug susceptibility rates were 100%. Conclusions The carrying rates of virulence genes of Vibrio parahaemolyticus isolated from patients with diarrhea in Ningbo are high. The drug susceptibility rates are high to common antibiotics,and it should also pay attention to monitoring drug resistance rate and guiding rational antibiotic use in clinic.

Key words: Vibrio parahaemolyticus, Diarrhea, Virulence gene, Drug resistance

CLC Number:

R378.3

XIE Hongyi, YE Hongyan, ZHOU Fangman, SHI Lumei, GE Tingting, LE Xubo. Virulence genes and drug resistance of Vibrio parahaemolyticus isolated from patients with diarrhea in Ningbo[J]. Laboratory Medicine, 2019, 34(2): 136-139.

Figures/Tables 3

References 16

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基因名称	序列（5'~3'）	长度（bp）	反应条件
tlh	AAAGCGGATTATGCAGAAGCACTG	450	94 ℃ 5 min; 94 ℃ l min,58 ℃ l min,72 ℃ l min, 30个循环;72 ℃ 10 min
	GCTACTTTCTAGCATTTTCTCTGC
trh	TTGGCTTCGATATTTTCAGTATCT	500	95 ℃ 5 min; 95 ℃ l min,54 ℃ l min,72 ℃ l min, 30个循环;72 ℃ 10 min
	CATAACAAACATATGCCCATTTCCG
tdh	GTAAAGGTCTCTGACTTTTGGAC	269	94 ℃ 5 min; 94 ℃ l min,58 ℃ l min,72 ℃ l min, 30个循环;72 ℃ 10 min
	TGGAATAGAACCTTCATCTTCACC

基因名称	序列（5'~3'）	长度（bp）	反应条件
tlh	AAAGCGGATTATGCAGAAGCACTG	450	94 ℃ 5 min; 94 ℃ l min,58 ℃ l min,72 ℃ l min, 30个循环;72 ℃ 10 min
	GCTACTTTCTAGCATTTTCTCTGC
trh	TTGGCTTCGATATTTTCAGTATCT	500	95 ℃ 5 min; 95 ℃ l min,54 ℃ l min,72 ℃ l min, 30个循环;72 ℃ 10 min
	CATAACAAACATATGCCCATTTCCG
tdh	GTAAAGGTCTCTGACTTTTGGAC	269	94 ℃ 5 min; 94 ℃ l min,58 ℃ l min,72 ℃ l min, 30个循环;72 ℃ 10 min
	TGGAATAGAACCTTCATCTTCACC

抗菌药物	敏感率	耐药率
头孢吡肟	100.0	0.0
左氧氟沙星	100.0	0.0
亚胺培南	100.0	0.0
美洛培南	100.0	0.0
哌拉西林-他唑巴坦	100.0	0.0
头孢他啶	97.8	0.0
头孢噻肟	93.9	0.0
四环素	91.8	0.0
氯霉素	89.8	1.6
庆大霉素	80.0	0.0
复方磺胺甲噁唑	75.5	1.5
头孢西丁	74.5	0.0
环丙沙星	74.5	2.7
头孢呋辛	26.5	20.4
阿米卡星	16.3	22.4
头孢唑啉	10.2	58.6
氨苄西林	10.2	75.5

抗菌药物	敏感率	耐药率
头孢吡肟	100.0	0.0
左氧氟沙星	100.0	0.0
亚胺培南	100.0	0.0
美洛培南	100.0	0.0
哌拉西林-他唑巴坦	100.0	0.0
头孢他啶	97.8	0.0
头孢噻肟	93.9	0.0
四环素	91.8	0.0
氯霉素	89.8	1.6
庆大霉素	80.0	0.0
复方磺胺甲噁唑	75.5	1.5
头孢西丁	74.5	0.0
环丙沙星	74.5	2.7
头孢呋辛	26.5	20.4
阿米卡星	16.3	22.4
头孢唑啉	10.2	58.6
氨苄西林	10.2	75.5