Abstract: Objective To investigate the clinical characteristics and epidemiological of enterovirus in children with acute diarrhea in Tianjin，and to provide a reference for the prevention and treatment of viral diarrhea. Methods The 1 102 stool samples of hospitalized children with acute diarrhea admitted in Tianjin Children's Hospital from August 2019 to July 2020 were collected，and there were 680 boys and 422 girls，aged 1-148 months. Rotavirus and adenovirus were determined by colloidal gold immunochromatography，and norovirus was determined by reverse transcription polymerase chain reaction（PCR）. The determination results and clinical data were collected and analyzed. Results Totally，421（38.2%）children were positive for viruses，and the determination rates of norovirus，rotavirus and adenovirus were 24.9%，11.0% and 2.4%，respectively. Norovirus and rotavirus had high determination rates in winter and spring，while adenovirus had high determination rates in summer and autumn. There was statistical significance in the determination rates of the 3 enteroviruses in different age groups（P<0.05），and there was statistical significance in the determination rates of norovirus and adenovirus between boys and girls（P<0.05）. The incidence rates of associated symptoms such as fever，cough，vomiting and abdominal pain of acute diarrhea caused by different enteroviruses were different（P<0.05）. The incidence rates of water，electrolyte and acid-base disturbance were higher in adenovirus infected children（P<0.05）. The incidence rate of fat droplets in stool routine examination and the incidence rate of leukopenia in peripheral blood were higher in rotavirus infected children（P<0.05）. Conclusions Norovirus and rotavirus are the main etiological factors of diarrhea among children in Tianjin and mostly occur in infants and young children with obvious seasonality. The clinical characteristics and laboratory examination results of diarrhea caused by different viral infections are different，which may provide a reference for the clinical treatment of diarrhea.

Key words: Enterovirus, Acute diarrhea, Epidemiology, Clinical characteristic, Children, Tianjin