Research progress of bacterial integron determination methods

doi:10.3969/j.issn.1673-8640.2022.09.019

Abstract

Abstract:

Bacterial integrons have unique structures that can capture and express foreign genes,and they can be transmitted horizontally between bacteria through transposons and plasmids. The emergence of multi-drug-resistant bacteria is a challenge for clinical treatment,and many drug-resistant bacteria carry integrons. Therefore,the early determination of integrons is important for the early determination of bacterial drug resistance. At present,the methods of bacterial integron determination are mainly to determine the integrase of integron and to determine gene cassettes. This review introduces several bacterial integron determination methods,including the determination methods based on polymerase chain reaction（PCR）,loop-mediated isothermal amplification（LAMP）,metagenomic next generation sequencing（mNGS）,gene chip technology. The principle and characteristics of various determination methods are introduced,and the advantages and disadvantages of methods are compared. This review provides a reference for finding a better determination method for clinical use,so as to grasp the situation of bacterial integron carrying in real time and respond to bacterial drug resistance as soon as possible.

Key words: Bacterial integron, Gene cassette, Integrase, Drug resistance

CLC Number:

R446.5

HAO Jiahui, YANG Zehua. Research progress of bacterial integron determination methods[J]. Laboratory Medicine, 2022, 37(9): 887-891.

References 36

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