Characteristics and whole-genome sequencing of 1 isolate of Campylobacter coil from blood

doi:10.3969/j.issn.1673-8640.2019.12.007

Abstract

Abstract:

Objective To investigate the characteristics and whole-genome sequencing of 1 isolate of Campylobacter coil from blood. Methods The isolate from an elder female blood was identified for type,drug susceptibility and gene sequence by routine biochemical method,mass spectrometry,genomic sequencing and so on. Results This Campylobacter coil was collected,which can survive for 3 months in blood culture bottle at room temperature,and it was sensitive to erythromycin,but it was resistant to ciprofloxacin and tetracycline. The data of whole-genome sequencing showed 1.86 Mb,the number of gene was 1 693. The sequence numbers of tRNA and rRNA were 3 and 40,respectively. A total of 21 groups of COG functions and 3 GO contents were obtained by comparing the annotations between COG database and GO database. The top 5 drug resistance genes were CmeB,TetO,CmeC,MacB and CmeA. The top 5 drug virulence genes were Cj1435c,fsrC,ECP_2806,lpg0769 and A1S-0053. Conclusions The isolate keeps high sensitive to erythromycin,but it is highly resistant to ciprofloxacin and tetracycline. There is a correlation between drug resistance and CmeB,TetO,CmeC,MacB,CmeA and so on. The virulence is related with Cj1435c,fsrC and so on.

Key words: Campylobacter coil, Blood infection, Biological characteristic, Whole-genome sequencing, Drug resistance, Drug resistance gene, Virulence gene

CLC Number:

R378.3

HUANG Chunlan, XU Qiuhong, YU Hongli, MA Limei, LIAO Zhihong. Characteristics and whole-genome sequencing of 1 isolate of Campylobacter coil from blood[J]. Laboratory Medicine, 2019, 34(12): 1091-1096.

Figures/Tables 6

References 14

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类型	测序数	测序长度（bp）
rRNA	40	3 155
tRNA	3	4 670

类型	测序数	测序长度（bp）
rRNA	40	3 155
tRNA	3	4 670

起始位点	终止位点	分值	正义链/反义链	类别
6205	6319	57.4	+	5s_rRNA
2814	5867	3141.9	+	23s_rRNA
439	1939	1762.9	+	16s_rRNA

起始位点	终止位点	分值	正义链/反义链	类别
6205	6319	57.4	+	5s_rRNA
2814	5867	3141.9	+	23s_rRNA
439	1939	1762.9	+	16s_rRNA

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