Inhibition and mechanism of tannic acid on biofilm formation in Klebsiella pneumoniae

doi:10.3969/j.issn.1673-8640.2021.010.017

Abstract

Abstract:

Objective To study the inhibition and its mechanism of tannic acid on biofilm formation in Klebsiella pneumoniae. Methods Totally,3 isolates of carbapenem-resistant Klebsiella pneumoniae（CRKP） and 1 sensitive isolate KP 13883 were chosen. Minimum inhibitory concentrations（MIC） of imipenem and meropenem were determined by broth micro-dilution method,and the biofilm formation ability was evaluated using 96-well plate crystal violet staining method with or without tannic acid. The morphological differences of biofilm were obtained by scanning electron microscopy before and after the treatment of tannic acid. Real-time fluorescence quantitative polymerase chain reaction（PCR） was used to determine the expression of biofilm-related genes（mrkA,luxS and wbbM） in the presence and absence of tannic acid. Results The 3 CRKP isolates showed decreased susceptibilities to imipenem and meropenem,and the MIC were ≥64 μg/mL. Tannic acid could decrease the growth and biofilm formation ability of CRKP（P<0.05）,which was further confirmed by scanning electron microscopy. The results of real-time fluorescence quantitative PCR showed that tannic acid could reduce the expression of quorum sensing related gene luxS and lipopolysaccharide related gene wbbM. Conclusions Tannic acid could decrease the biofilm formation ability of CRKP.

Key words: Klebsiella pneumoniae, Biofilm, Tannic acid, Lipopolysaccharide

CLC Number:

R446.1

ZHOU Yanxia, JIN Dongfang. Inhibition and mechanism of tannic acid on biofilm formation in Klebsiella pneumoniae[J]. Laboratory Medicine, 2021, 36(10): 1070-1073.

Figures/Tables 5

References 12

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引物名称	引物序列（5'~3'）	引物大小/bp
mrkA-F	ACGTCTCTAACTGCCAGGC	115
mrkA-R	TAGCCCTGTTGTTTGCTGGT
luxS-F	AGTGATGCCGGAACGCGG	148
luxS-R	CGGCGTACCAATCAGGCTC
wbbM-F	ATGCGGGTGAGAACAAACCA	124
wbbM-R	AGCCGCTAACGACATCTGAC
rpoB-F	AGGCGAATCCAGCTTGTTCAGC	147
rpoB-R	TGACGTTGCATGTTCGCACCCATCA

引物名称	引物序列（5'~3'）	引物大小/bp
mrkA-F	ACGTCTCTAACTGCCAGGC	115
mrkA-R	TAGCCCTGTTGTTTGCTGGT
luxS-F	AGTGATGCCGGAACGCGG	148
luxS-R	CGGCGTACCAATCAGGCTC
wbbM-F	ATGCGGGTGAGAACAAACCA	124
wbbM-R	AGCCGCTAACGACATCTGAC
rpoB-F	AGGCGAATCCAGCTTGTTCAGC	147
rpoB-R	TGACGTTGCATGTTCGCACCCATCA

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