Abstract: Objective　To investigate the species and distribution of pathogens in the vaginal secretion of patients with recurrent vaginitis，and to provide reference for clinical application.  Methods　The vaginal secretion samples were collected from the outpatients with vaginitis in the Department of Gynecology. The normal saline smear method was used to detect the trichomonas，rapid Gram′s staining was used to detect Candida pelliculosa，and sialidase method was used to detect bacterial vaginosis (BV). Results　In 67 patients with recurrent vaginitis，32 cases with pathogen infection were detected(47.76%)，and 19 cases with single pathogen infection were detected (28.36%)，including 10 cases of single Candida pelliculosa infection (14.93%)，1 case of single trichomonas infection (1.49%) and 8 cases of single positive BV (11.94%). A total of 13 cases of mixed infection were detected (19.40%)，among which Candida pelliculosa mixing with positive BV had 10 cases (14.93%)，trichomonas mixing with positive BV had 2 cases (2.99%), and Candida pelliculosa and trichomonas mixing with positive BV had 1 case (1.49%). In 123 cases of control group，50 cases were found with pathogen infection (40.65%), including 40 single infection cases(32.52%) and 10 mixed infection cases(8.13%). Among the 40 single infection cases，single Candida pelliculosa infection was found in 18 cases(14.63%)，single trichomonas infection was found in 2 cases (1.63%)，and single positive BV was found in 20 cases(16.26%). Among the 10 mixed infection cases，Candida pelliculosa mixing with positive BV had 8 cases (6.50%)，and trichomonas mixing with positive BV had 2 cases (1.63%). Conclusions　The pathogen mixed infection in patients with recurrent vaginitis is notable，accounting for nearly half of the detected pathogens，especially the cases mixing with BV，and it should arouse the notice in clinical application.

Key words: Vaginitis, Candida pelliculosa, Bacterial vaginosis, Trichomonas