›› 2013, Vol. 28 ›› Issue (4): 296-300.DOI: 10.3969/j.issn.1673-8640.2013.04.011

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Establishment and verification of blood cell analysis review criteria in women and children specialized hospital

  

  1. Department of Clinical Laboratory,West China Second Hospital,Sichuan University,Sichuan Chengdu 610041,China
  • Received:2012-03-13 Revised:2012-07-26 Online:2013-04-30 Published:2013-04-05

Abstract: Objective To establish proper review criteria for blood cell analysis in women and children specialized hospital. Methods Based on the review criteria from the cooperation group of formulation for blood cell analysis by Sysmex XE-2100 hematology analyzer,as the women and children specialized hospital with the own unique disease characteristics and children white blood cell (WBC) physiological characteristics,the review criteria was optimized. The total 1 120 samples were detected by Sysmex XE-2100 hematology analyzer, blood smear determination was as the golden standard,and the new review criteria was evaluated and optimized. Results The review rate of initial review criteria was 65.98%. According to the women and children specialized hospital characteristics,by adjusting the WBC,platelet (PLT) count and WBC differential percentage for the first time after optimization,the false positive rate was 40.71%. By combining immature granulocyte (IG) and WBC≥10×109/L,combining blast cell (Blast) and WBC≤4×109/L or ≥10×109/L,and combining atypical/variant lymphocyte (Lymph) (Atypical/Variant) and Lymph%>50% for the second time after optimization,the false positive and false negative rates were 17.53% and 4.49%. The main items were Atypical/Variant Lymph (<5%)and PLT>70×109/L,which were verified. The proper review criteria in women and children specialized hospital was established. Conclusions The review criteria for women and children specialized hospital should be established in clinical application,improve the review criteria timely,and improve the analysis quality and efficiency of blood cell analysis.

Key words: Review criteria, Blood cell count, Auto-analysis