Abstract: Objective　To observe the change of immune function in patients with chronic hepatitis B, and to investigate the relationship of interferon-gamma(IFN-γ), interleukin 10 (IL-10) and CD19^＋ with hepatitis B virus（HBV） DNA load.   Methods　By enzyme-linked immunosorbent assay (ELISA) and flow cytometry, the serum IFN-γ, IL-10 and peripheral blood CD19^＋ levels of 76 patients with chronic hepatitis B and 30 healthy subjects were determined. By fluorescence quantitative polymerase chain reaction (PCR), serum HBV DNA copy number was determined. Results　The IFN-γ levels in HBV DNA low copy group and high copy group decreased significantly compared with those in HBV DNA negative group. Their IL-10 and CD19^＋ levels increased significantly compared with those in HBV DNA negative group. The IFN-γ levels were correlated negatively with the HBV DNA load (r＝－0.311,P＜0.05)， and the IL-10 and CD19^＋ levels were correlated positively with the HBV DNA load (r＝0.350,P＜0.05; r＝0.321, P＜0.05).  Conclusions　The patients have immune function adjustment disorder. The IFN-γ, IL-10 and CD19^＋ levels can be monitoring indices for the inflammatory of hepatitis B related diseases.

Key words: Interferon-gamma, Interleukin 10, CD19, DNA, Hepatitis B virus, Chronic hepatitis B