Characteristic of blood microbiota of postoperative sepsis based on cfDNA NGS

doi:10.3969/j.issn.1673-8640.2023.08.009

Abstract

Abstract:

Objective To analyze the characteristic of blood microbiota in patients with sepsis after operation. Methods The sequencing data of microbial cell-free DNA（cfDNA） from plasma of 239 postoperative sepsis patients（SP）（SP group） and 34 postoperative patients without SP [control patients（CP）（CP group）] were downloaded from SRA-NCBI databases and reanalyzed by bioinformatics. Results The blood microbiota in SP group showed a decreased microbial diversity compared with CP group，which mainly enriched in 12 taxa. The most representative taxa which were increased in SP group，with higher relative abundance，included Stenotrophomonas maltophilia，Rhodococcus erythropolis，Acidovorax sp. KKS102，Ralstonia pickettii，Enterococcus faecium and human beta herpes virus 5. Conclusions The composition of blood microbiota is altered in SP group compared with CP group，and the 6 representative taxa may play roles in the development of SP.

Key words: Sepsis, Postoperative complication, Cell-free DNA, Microbial composition

CLC Number:

R446.1

LIANG Dong, MEI Yue, WANG Tengjiao, YU Dong. Characteristic of blood microbiota of postoperative sepsis based on cfDNA NGS[J]. Laboratory Medicine, 2023, 38(8): 753-759.

Figures/Tables 6

References 40

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微生物名称	CP/%	SP/%	P值
革兰阴性菌
麦芽寡养单胞菌	14.590	48.810	1.01×10^-60
食酸菌sp. KKS102	0.470	2.900	1.04×10^-56
皮氏罗尔斯顿菌	0.930	2.540	1.16×10^-35
屎肠球菌	0.002	2.530	1.92×10^-3
红串红球菌	0.053	14.170	1.71×10^-59
人乙型疱疹病毒5	<0.001	1.490	0.014

微生物名称	CP/%	SP/%	P值
革兰阴性菌
麦芽寡养单胞菌	14.590	48.810	1.01×10^-60
食酸菌sp. KKS102	0.470	2.900	1.04×10^-56
皮氏罗尔斯顿菌	0.930	2.540	1.16×10^-35
屎肠球菌	0.002	2.530	1.92×10^-3
红串红球菌	0.053	14.170	1.71×10^-59
人乙型疱疹病毒5	<0.001	1.490	0.014

微生物名称	血培养	NGS	本研究
前期研究^[15-16]
光滑念珠菌	+
鼠李糖乳酸杆菌	+		+
副干酪乳杆菌	+		+
经黏液真杆菌.	+		+
金黄色葡萄球菌	+		+
阴沟肠杆菌	+		+
普通拟杆菌		+	+
肺炎克雷伯菌		+	+
肠道沙门菌		+	+
假单胞菌属门多萨菌		+	+
多形拟杆菌		+	+
大肠埃希菌	+	+	+
白念珠菌	+	+
脆弱拟杆菌	+	+	+
本研究
屎肠球菌	+	+	+
人乙型疱疹病毒5		+	+
麦芽寡养单胞菌			+
食酸菌sp. KKS102			+
红串红球菌			+
皮氏罗尔斯顿菌			+

微生物名称	血培养	NGS	本研究
前期研究^[15-16]
光滑念珠菌	+
鼠李糖乳酸杆菌	+		+
副干酪乳杆菌	+		+
经黏液真杆菌.	+		+
金黄色葡萄球菌	+		+
阴沟肠杆菌	+		+
普通拟杆菌		+	+
肺炎克雷伯菌		+	+
肠道沙门菌		+	+
假单胞菌属门多萨菌		+	+
多形拟杆菌		+	+
大肠埃希菌	+	+	+
白念珠菌	+	+
脆弱拟杆菌	+	+	+
本研究
屎肠球菌	+	+	+
人乙型疱疹病毒5		+	+
麦芽寡养单胞菌			+
食酸菌sp. KKS102			+
红串红球菌			+
皮氏罗尔斯顿菌			+