Expression levels of microRNA-132 and high-mobility group box-1 protein in peripheral blood mononuclear cells of children with sepsis

doi:10.3969/j.issn.1673-8640.2018.09.010

Abstract

Abstract:

Objective To investigate the expression levels of microRNA-132（miR-132） and high-mobility group box-1 protein（HMGB1） in peripheral blood mononuclear cells of children with sepsis. Methods A total of 87 children with sepsis were enrolled. According to prognosis,the children were classified into death group （25 cases） and non-death group （62 cases）. A total of 50 healthy children were enrolled as control group. Sepsis group was determined within 12 h after diagnosis,and control group was determined at physical examination day. Real-time fluorescence quantitation polymerase chain reaction （PCR）was used to determine the expression levels of miR-132 and HMGB1 in peripheral blood mononuclear cells. The roles of miR-132 and HMGB1 in the prognosis of children were analyzed using receiver operating characteristic （ROC） curve. Results The relative expression levels of miR-132 and HMGB1 mRNA in peripheral blood mononuclear cells in sepsis group were higher than those in control group （P<0.05）. The relative expression levels of miR-132 and HMGB1 mRNA in peripheral blood mononuclear cells in death group were higher than those in non-death group （P<0.05）. Pearson correlation analysis showed that the relative expression level of miR-132 in peripheral blood mononuclear cells of sepsis group was positively correlated with that of HMGB1 mRNA （r=0.521,P<0.001）. ROC curve analysis showed that using the relative expression levels of miR-132 and HMGB1 mRNA in predicting the prognosis of children,the areas under ROC curves （AUC） were 0.921 [95% confidence interval（CI） 0.853-0.989] and 0.858 （95%CI 0.769-0.947）,the sensitivities were 84.0% and 68.0%,and the specificities were 87.1% and 90.3%,respectively. The AUC of the combined determination of miR-132 and HMGB1 mRNA was 0.941 （95%CI 0.878-1.000）,the sensitivity was 88.0%,and the specificity was 91.9%. Conclusions The expression levels of miR-132 and HMGB1 in peripheral blood mononuclear cells of children with sepsis are increased,and they are related to the prognosis of children. They could be used as early assessment indices for the prognosis of children.

Key words: MicroRNA-132, High-mobility group box-1 protein, Sepsis, Peripheral blood mononuclear cell, Expression

CLC Number:

R446.1

ZHANG Cui. Expression levels of microRNA-132 and high-mobility group box-1 protein in peripheral blood mononuclear cells of children with sepsis[J]. Laboratory Medicine, 2018, 33(9): 815-818.

Figures/Tables 2

References 14

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组别	例数	miR-132	HMGB1 mRNA
脓毒症患儿	87	1.59±0.24^*	1.63±0.11^*
死亡组	25	1.90±0.21^*△	1.71±0.12^*△
非死亡组	62	1.47±0.11^*	1.59±0.09^*
对照组	50	1.27±0.14	1.12±0.08

组别	例数	miR-132	HMGB1 mRNA
脓毒症患儿	87	1.59±0.24^*	1.63±0.11^*
死亡组	25	1.90±0.21^*△	1.71±0.12^*△
非死亡组	62	1.47±0.11^*	1.59±0.09^*
对照组	50	1.27±0.14	1.12±0.08

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