Relationship between the expression of miR-138 in peripheral blood mononuclear cells of hepatitis B virus proliferation in carriers

doi:10.3969/j.issn.1673-8640.2023.06.010

Abstract

Abstract:

Objective To investigate the correlation between the expression of miR-138 in peripheral blood mononuclear cells（PBMC） and hepatitis B virus（HBV）proliferation in HBV carriers. Methods A total of 144 HBV carriers（HBV carrier group） and 144 healthy subjects（healthy control group） were enrolled from Dongying People's Hospital from January 2018 to January 2021. The general data，such as sex and age，hepatitis B serological markers and liver function determination results were collected. The relative expression of miR-138 in PBMC was determined，and the HBV DNA load of HBV carriers was determined. According to the results of hepatitis B serological markers，HBV carriers were classified into hepatitis B e antigen（HBeAg） negative group（63 cases） and HBeAg positive group（81 cases）. Pearson correlation analysis was used to evaluate the correlation between the relative expression of miR-138 and HBV DNA load. Results The relative expression of miR-138 in PBMC of HBV carrier group was lower than that of healthy control group（P<0.001）. The HBV DNA load in HBeAg positive group was higher than that in HBeAg negative group（P<0.001），and the relative expression of miR-138 in PBMC was lower than that in HBeAg negative group（P<0.001）. Pearson correlation analysis showed that PBMC miR-138 relative expression was negatively correlated with HBV DNA load in HBV carrier group（r=-0.841，P<0.05）. Conclusions The expression of miR-138 in PBMC of HBV carriers is down-regulated，which is related to HBV proliferation.

Key words: MicroRNA-138, Mononuclear cell, Hepatitis B virus, Proliferation

CLC Number:

R446.1

ZHANG Ying, JING Lijuan, LI Li. Relationship between the expression of miR-138 in peripheral blood mononuclear cells of hepatitis B virus proliferation in carriers[J]. Laboratory Medicine, 2023, 38(6): 559-562.

Figures/Tables 4

References 18

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引物名称	上游引物序列（5'~3'）	下游引物序列（5'~3'）
miR-138	GGTGTCGTGGAGTCGGCAA	AACTTCACAACACCAGCTTA
U6	CTCGCTTCGGCAGCACA	AACGCTTCACGAATTTGCGT

引物名称	上游引物序列（5'~3'）	下游引物序列（5'~3'）
miR-138	GGTGTCGTGGAGTCGGCAA	AACTTCACAACACCAGCTTA
U6	CTCGCTTCGGCAGCACA	AACGCTTCACGAATTTGCGT

组别	例数	ALT/ （U/L）	AST/ （U/L）	TB/ （μmol/L）
HBV携带组	144	84.37±15.18	89.72±26.32	31.29±3.74
正常对照组	144	23.09±11.34	24.77±11.59	12.59±1.83
t值		38.809	40.811	53.894
P值		<0.001	<0.001	<0.001

组别	例数	ALT/ （U/L）	AST/ （U/L）	TB/ （μmol/L）
HBV携带组	144	84.37±15.18	89.72±26.32	31.29±3.74
正常对照组	144	23.09±11.34	24.77±11.59	12.59±1.83
t值		38.809	40.811	53.894
P值		<0.001	<0.001	<0.001

组别	例数	miR-138	HBV DNA
HBeAg阳性组	81	1.05±0.14	6.22±0.48
HBeAg阴性组	63	1.24±0.17	5.73±0.29
t值		10.353	7.580
P值		<0.001	<0.001