Expression of RRBP1 in osteosarcoma tissues and its effect on cell biological characteristics

doi:10.3969/j.issn.1673-8640.2023.09.006

Abstract

Abstract:

Objective To investigate the expression of ribosome-binding protein 1（RRBP1） in osteosarcoma tissues and the effect on the cell biological characteristics of osteosarcoma U-2OS cells. Methods A total of 91 patients with osteosarcoma undergoing surgical treatment in Nanyang Second People's Hospital and the First Affiliated Hospital of Zhengzhou University from June 2014 to June 2020 were enrolled，and their osteosarcoma tissues were collected. Totally，45 patients who underwent orthopedic surgery and excluded bone tumors were enrolled，and the normal bone tissues were collected. The expressions of RRBP1 proteins in osteosarcoma tissues and normal bone tissues were determined by immunohistochemical method. The patients with osteosarcoma were followed up after surgical treatment，with a follow-up until December 2021，and the survival was recorded. The osteosarcoma cell line U-2OS was cultured and classified into si-RRBP1 group（transfected with RRBP1 interference sequence），si-NC group（transfected with negative control sequence） and blank group（only added with transfection reagent）. Normal cultured U-2OS cells were used as control group. The expressions of RRBP1 mRNA and protein in each group were determined，and the proliferative activity，migration and invasion ability of cells were determined. Results The positive expression rate of RRBP1 protein in osteosarcoma tissues was higher than that in normal bone tissues（P<0.001）. The positive rates of RRBP1 protein in osteosarcoma patients with Enneking stage ⅡB to Ⅲ and distant metastasis were higher than those in patients with Enneking stage ⅡA and without distant metastasis（P<0.05）. There was no statistical significance in the positive rates of RRBP1 protein among osteosarcoma patients with different sex，ages，tumor locations，histological types and tumor sizes（P>0.05）. The survival rate and time of patients in RRBP1 positive group were lower than those in RRBP1 negative group（P=0.003）. The relative expressions of RRBP1 mRNA and protein in si-RRBP1 group is cell were lower than those in si-NC groups，blank groups and U-2OS （P<0.05）. Compared with si-NC group and blank group，the absorbance（A） values in si-RRBP1 group at 24，48，72 and 96 h were decreased（P<0.05）. The number of migrating cells and invasive cells in si-RRBP1 group were lower than those in si-NC group and blank group（P<0.05）. Conclusions The expression of RRBP1 protein in osteosarcoma tissues is highly expressed，and it is related to patient prognosis. Down-regulated RRBP1 gene expression in osteosarcoma U-2OS cells can inhibit cell proliferative activity and inhibit cell migration and invasion.

Key words: Ribosome-binding protein 1, Proliferation, Metastasis, Invasion, Osteosarcoma

CLC Number:

R393

YANG Yuqiang, QUAN Xiaoli, WANG Liuyu, XIAN Wenfeng, YANG Hong. Expression of RRBP1 in osteosarcoma tissues and its effect on cell biological characteristics[J]. Laboratory Medicine, 2023, 38(9): 842-848.

Figures/Tables 10

References 22

[1]	孙小丹, 刘鷖雯, 何怡青, 等. 雌激素受体在骨肉瘤中的作用研究进展[J]. 检验医学, 2022, 37(4):313-318.
[2]	汪勇刚. 骨肉瘤中miR-194的表达水平及其与临床病理参数的关系[J]. 临床骨科杂志, 2020, 23(2):280-283.
[3]	缪明星, 黄莉莉, 王欣. 紫草素通过内质网应激蛋白抑制人骨肉瘤U-2 OS细胞增殖的研究[J]. 现代药物与临床, 2019, 34(10):2899-2904.
[4]	WANG Z, YIN F, XU J, et al. CYT997(lexibulin) induces apoptosis and autophagy through the activation of mutually reinforced ER stress and ROS in osteosarcoma[J]. J Exp Clin Cancer Res, 2019, 38(1):44. DOI PMID
[5]	ZHU J, ZHAO R, XU W, et al. Correlation between reticulum ribosome-binding protein 1(RRBP1) overexpression and prognosis in cervical squamous cell carcinoma[J]. Biosci Trends, 2020, 14(4):279-284. DOI URL
[6]	姬红英, 马荣. RRBP1表达与相关肿瘤预后关系的研究进展[J]. 肿瘤学杂志, 2019, 25(11):1001-1005.
[7]	LI T, WANG Q, HONG X, et al. RRBP1 is highly expressed in prostate cancer and correlates with prognosis[J]. Cancer Manag Res, 2019, 11:3021-3027. DOI PMID
[8]	WANG L, WANG M, ZHANG M, et al. Expression and significance of RRBP1 in esophageal carcinoma[J]. Cancer Manag Res, 2018, 10:1243-1249. DOI PMID
[9]	丁丽君, 田洁, 李晓兰. LAPTM4B在子宫内膜癌组织中表达及临床意义[J]. 昆明医科大学学报, 2019, 40(3):87-91.
[10]	吴鸿斌, 孙红, 彭国璇, 等. 长链非编码RNA SNHG家族分子在骨肉瘤中的发生机制及预后价值[J]. 中华实验外科杂志, 2020, 37(11):2127-2130.
[11]	何军, 王明海, 柴宇啸. 四肢骨肉瘤保肢治疗与预后影响因素分析[J]. 癌症进展, 2021, 19(2):157-159.
[12]	董孟权, 杨丽英. 核糖体结合蛋白在甲状腺癌中的表达及其与临床病理特征的关系[J]. 蚌埠医学院学报, 2016, 41(2):215-216.
[13]	王博, 任晓俊, 薛誉, 等. 维替泊芬诱导子宫内膜癌细胞发生内质网应激及自噬相关蛋白表达[J]. 复旦学报(医学版), 2021, 48(1):8-16.
[14]	RANA S V S. Endoplasmic reticulum stress induced by toxic elements-a review of recent developments[J]. Biol Trace Elem Res, 2020, 196(1):10-19. DOI PMID
[15]	郭茜晨, 张婷婷, 王润新, 等. 内质网应激反应IRE1α信号通路与细胞衰老在癌症中的作用及关系的研究进展[J]. 癌变·畸变·突变, 2021, 33(1):72-76.
[16]	KERR S R, KATZ S G. Activation of the unfolded protein response pathway in cytotoxic T cells:a comparison between in vitro stimulation,infection,and the tumor microenvironment[J]. Yale J Biol Med, 2019, 92(4):675-685.
[17]	彭彦才, 郝登荣. 核糖体结合蛋白1在食管癌中的表达及其临床意义[J]. 国际肿瘤学杂志, 2019, 46(11):668-672.
[18]	LIU S, LIN M, JI H, et al. RRBP1 overexpression is associated with progression and prognosis in endometrial endometrioid adenocarcinoma[J]. Diagn Pathol, 2019, 14(1):7. DOI PMID
[19]	LV S W, SHI Z G, WANG X H, et al. Ribosome binding protein 1 correlates with prognosis and cell proliferation in bladder cancer[J]. Onco Targets Ther, 2020, 13:6699-6707. DOI URL
[20]	廖代祥, 郑博, 张彦斌, 等. RRBP1与RABEX-5蛋白在结直肠癌组织中的表达及与临床病理特征和预后的关系[J]. 实用肿瘤学杂志, 2020, 34(1):43-48.
[21]	HE Y, HUANG S, CHENG T, et al. High glucose may promote the proliferation and metastasis of hepatocellular carcinoma via E2F1/RRBP1 pathway[J]. Life Sci, 2020, 252:117656. DOI URL
[22]	WANG M, LIU S, ZHOU B, et al. RRBP1 is highly expressed in bladder cancer and is associated with migration and invasion[J]. Oncol Lett, 2020, 20(5):203. DOI PMID

指标	例数	RRBP1阳性/[例（%）]
性别
男性	52	40（76.92）
女性	39	28（71.79）
χ²值		0.310
P值		0.577
年龄
≤20岁	64	50（78.13）
>20岁	27	18（66.67）
χ²值		1.320
P值		0.251
肿瘤部位
股骨和胫骨	72	55（76.39）
其他部位	19	13（68.42）
χ²值		0.505
P值		0.477
组织学类型
骨母细胞型	43	34（79.07）
软骨母细胞型	17	12（70.59）
纤维母细胞型	15	11（73.33）
混合型	16	11（68.75）
χ²值		0.902
P值		0.825
肿瘤大小
<5 cm	42	29（69.05）
≥5 cm	49	39（79.59）
χ²值		1.331
P值		0.249
Enneking分期
ⅡA期	28	16（57.14）
ⅡB~Ⅲ期	63	52（82.54）
χ²值		6.620
P值		0.010
远隔转移
有	42	36（85.71）
无	49	32（65.31）
χ²值		4.987
P值		0.026

指标	例数	RRBP1阳性/[例（%）]
性别
男性	52	40（76.92）
女性	39	28（71.79）
χ²值		0.310
P值		0.577
年龄
≤20岁	64	50（78.13）
>20岁	27	18（66.67）
χ²值		1.320
P值		0.251
肿瘤部位
股骨和胫骨	72	55（76.39）
其他部位	19	13（68.42）
χ²值		0.505
P值		0.477
组织学类型
骨母细胞型	43	34（79.07）
软骨母细胞型	17	12（70.59）
纤维母细胞型	15	11（73.33）
混合型	16	11（68.75）
χ²值		0.902
P值		0.825
肿瘤大小
<5 cm	42	29（69.05）
≥5 cm	49	39（79.59）
χ²值		1.331
P值		0.249
Enneking分期
ⅡA期	28	16（57.14）
ⅡB~Ⅲ期	63	52（82.54）
χ²值		6.620
P值		0.010
远隔转移
有	42	36（85.71）
无	49	32（65.31）
χ²值		4.987
P值		0.026

组别	12 h	24 h	48 h	72 h	96 h
si-RRBP1组	0.32±0.05	0.48±0.08	0.63±0.10	0.84±0.08	0.93±0.13
si-NC组	0.28±0.06	0.60±0.07^*	0.82±0.08^*	1.05±0.09^*	1.29±0.06^*
空白对照组	0.29±0.07	0.69±0.08^*	0.84±0.11^*	1.08±0.11^*	1.24±0.08^*
F值	0.486	11.049	8.696	11.630	24.960
P值	0.625	0.001	0.003	0.001	<0.001

组别	12 h	24 h	48 h	72 h	96 h
si-RRBP1组	0.32±0.05	0.48±0.08	0.63±0.10	0.84±0.08	0.93±0.13
si-NC组	0.28±0.06	0.60±0.07^*	0.82±0.08^*	1.05±0.09^*	1.29±0.06^*
空白对照组	0.29±0.07	0.69±0.08^*	0.84±0.11^*	1.08±0.11^*	1.24±0.08^*
F值	0.486	11.049	8.696	11.630	24.960
P值	0.625	0.001	0.003	0.001	<0.001

组别	迁移细胞数	侵袭细胞数
si-RRBP1组	103.67±9.63	88.50±9.33
si-NC组	136.00±6.03^*	119.83±6.18^*
空白对照组	127.50±7.40^*	118.50±6.44^*
F值	27.519	33.884
P值	<0.001	<0.001