Abstract:

Objective To investigate the effect of polypyrimidine tract-binding protein 1（PTBP1） on the proliferation activity，migration and invasion abilities of choroidal melanoma（CM） cells. Methods Human CM cell line OCM-1 was selected and classified into si-PTBP1 group（transfected with PTBP1 specific interference sequence），si-Control group（transfected with negative control sequence） and blank group（without any treatment），according to different transfection sequences. The expressions of PTBP1 mRNA，PTBP1，E-cadherin（E-Cad） and vimentin protein were determined. The proliferation activity，migration and invasion abilities were determined by cytological experiment. Results The relative expression level of PTBP1 mRNA in si-PTBP1 group was lower than those in si-Control group and blank group（P<0.05），and there was no statistical significance between si-Control group and blank group（P>0.05）. At 24，48，72 and 96 h of culture，the proliferation activities in si-PTBP1 group were lower than those in si-Control group and blank group（P<0.05）. The number of migrating cells and the number of invasive cells in si-PTBP1 group were lower than those in si-Control group and blank group（P<0.05）. The relative expression levels of PTBP1 and vimentin protein in si-PTBP1 group were lower than those in si-Control group and blank group（P<0.05），and the relative expression level of E-Cad was higher than those in si-Control group and blank group（P<0.05）. Conclusions The inhibition of the expression of PTBP1 in OCM-1 can inhibit cell proliferation activity and reduce migration and invasion，and its mechanism may be related to the inhibition of epithelial-mesenchymal transformation.

Key words: Polypyrimidine tract-binding protein 1, Cell proliferation, Cell invasion, Epithelial-mesenchymal transformation, Choroidal melanoma