Research on the function of miRNA-195 on bladder cancer cell

doi:10.3969/j.issn.1673-8640.2014.05.024

Abstract

Abstract: Objective To investigate the influence of microRNA (miRNA)-195 on the proliferation, migration and invasion of human bladder cancer cell 5637. Methods Human bladder cancer cell 5637 was transfected with miRNA-195 by cationic liposomes as miRNA-195 group and transfected with the vector as control group. The expression of miRNA-195 was determined by real-time fluorescence quantitation polymerase chain reaction (PCR). Methyl thiazolyl tetrazolium(MTT) assay, cell counting assay and colony formation assay were used to detect the cell proliferation . Cell migration and invasion were determined by wound healling assay, Transwell assay and Matrigel tumour invasion assay. Results Real-time fluorescence quantitation PCR showed that there was high expression of miRNA-195 after transfection of cell 5637. MTT assay, cell counting assay and colony formation assay demonstrated that miRNA-195 can suppress cell 5637 proliferation in vitro, and there were statistical significance between miRNA-195 and control groups(P<0.05).Wound healing assay, Transwell assay and tumour invasion assay showed that miRNA-195 can suppress cell migration and invasion with statistical significance between the 2 groups(P<0.05). Conclusions MiRNA-195 has inhibitory ability on the proliferation, migration and invasion of bladder cancer cell 5637 cell.

Key words: Micro RNA-195, Human bladder cancer cell, Cell proliferation, Cell migration

CLC Number:

CHEN Jing, SUN Fenyong, MA Ji.. Research on the function of miRNA-195 on bladder cancer cell[J]. , 2014, 29(5): 540-544.

References

[1] Van Roekel EH, Cheng KK, James ND, et al. Smoking is associated with lower age, higher grade, higher stage, and larger size of malignant bladder tumors at diagnosis[J]. Int J Cancer, 2013, 133(2):446-454.
[2] Kiriluk KJ, Prasad SM, Patel AR, et al. Bladder cancer risk from occupational and environmental exposures[J]. Urol Oncol, 2012, 30(2):199-211.
[3] Catto JW, Alcaraz A, Bjartell AS, et al. MicroRNA in prostate, bladder, and kidney cancer: a systematic review[J]. Eur Urol, 2011, 59(5):671-681.
[4] Bo J, Yang G, Huo K, et al. microRNA-203 suppresses bladder cancer development by repressing bcl-w expression[J]. FEBS J, 2011, 278(5):786-792.
[5] Majid S, Dar AA, Saini S, et al. MicroRNA-23b functions as a tumor suppressor by regulating Zeb1 in bladder cancer[J]. PLoS One, 2013, 8(7):e67686.
[6] Xu X, Chen H, Lin Y, et al. MicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met[J]. Mol Cells, 2013, 36(1):62-68.
[7] Hui W, Yuntao L, Lun L, et al.MicroRNA-195 inhibits the proliferation of human glioma cells by directly targeting cyclin D1 and cyclin E1[J]. PLoS One, 2013, 8(1):e54932.
[8] Deng H, Guo Y, Song H, et al.MicroRNA-195 and microRNA-378 mediate tumor growth suppression by epigenetical regulation in gastric cancer[J]. Gene, 2013, 518(2):351-359.
[9] Zaravinos A, Radojicic J, Lambrou GI, et al.Expression of miRNAs involved in angiogenesis, tumor cell proliferation, tumor suppressor inhibition, epithelial-mesenchymal transition and activation of metastasis in bladder cancer[J]. J Urol, 2012, 188(2):615-623.
[10] Guancial EA, Bellmunt J, Yeh S, et al. The evolving understanding of microRNA in bladder cancer[J]. Urol Oncol, 2013, 32(1):e31-e40.
[11] Wang R, Zhao N, Li S, et al.MicroRNA-195 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting the expression of VEGF, VAV2, and CDC42[J]. Hepatology, 2013, 58(2):642-653.
[12] Mao JH, Zhou RP, Peng AF, et al. microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN[J]. Oncol Lett, 2012, 4(5):1125-1129.
[13] 蒋海锋, 薄隽杰. MicroRNA与膀胱癌的研究进展[J]. 中国癌症杂志, 2011, 21(4): 308-312.
[14] Ichimi T, Enokida H, Okuno Y, et al. Identification of novel microRNA targets based on microRNA signatures in bladder cancer[J]. Int J Cancer, 2009, 125(2): 345-352.
[15] Huang L, Luo J, Cai Q, et al. MicroRNA-125b suppresses the development of bladder cancer by targeting E2F3[J]. Int J Cancer, 2011, 28(8): 1758-1769.
[16] Lu Q, Lu C, Zhou GP, et al. MicroRNA-221 silencing predisposed human bladder cancer cells to undergo apoptosis induced by TRAIL[J]. Urol Oncol, 2010, 28(6): 635-641.