Influence of MUC16 on the cell proliferation and invasion of osteosarcoma cells

doi:10.3969/j.issn.1673-8640.2022.010.014

Abstract

Abstract:

Objective To investigate the influence of mucin 16（MUC16） on the proliferation and invasion of human osteosarcoma cell line U2OS. Methods The U2OS cells were classified into sh-MUC16 group（transfected with MUC16 interference sequence），sh-Control group（transfected with negative control sequence） and blank group（without any treatment）. Real-time fluorescence quantitative polymerase chain reaction（PCR） was used to determine the expression of MUC16 mRNA in each group，methyl thiazolyl tetrazolium（MTT） method was used to determine cell proliferation activity，Transwell method was used to determine cell migration and invasion ability，and western blotting was used to determine the expressions of MUC16 epithelial E-cadherin（E-Cad） and neuropathic cadherin（N-Cad） in cells. Results The relative expression level of MUC16 mRNA in sh-MUC16 group were lower than those in sh-Control group and blank group（P<0.05），while there was no statistical significance between sh-Control group and blank group（P>0.05）. The cell proliferation activities in sh-MUC16 group at 24，48，72 and 96 h were lower than those in sh-Control group and blank group（P<0.05）. The number of migrating and invasive cells in sh-MUC16 group were lower than those in sh-Control group and blank group（P<0.05）. The relative expression levels of MUC16 and N-Cad in sh-MUC16 group were lower than those in sh-Control group and blank group，while the relative expression level of E-Cad was higher than those in sh-Control group and blank group（P<0.05）. Conclusions Silencing the expression of MUC16 gene in U2OS cells can inhibit cell proliferation activity，cell migration and invasion，and its mechanism may be related to the inhibition of epithelial-mesenchymal transition.

Key words: Mucin 16, Osteosarcoma, Cell proliferation, Cell invasion, Epithelial-mesenchymal transition

LI Mingwu, WANG Qinzhi, SUN Farui. Influence of MUC16 on the cell proliferation and invasion of osteosarcoma cells[J]. Laboratory Medicine, 2022, 37(10): 979-983.

Figures/Tables 7

References 19

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组别	培养12 h	培养24 h	培养48 h	培养72 h	培养96 h
sh-MUC16组	0.15±0.06	0.20±0.07^*#	0.25±0.07^*#	0.36±0.08^*#	0.50±0.05^*#
sh-Control组	0.18±0.05	0.34±0.07	0.39±0.03	0.57±0.04	0.75±0.07
空白组	0.15±0.03	0.35±0.11	0.42±0.12	0.63±0.10	0.73±0.05
F值	0.873	6.170	5.087	19.958	35.958
P值	0.438	0.011	0.021	<0.001	<0.001

组别	培养12 h	培养24 h	培养48 h	培养72 h	培养96 h
sh-MUC16组	0.15±0.06	0.20±0.07^*#	0.25±0.07^*#	0.36±0.08^*#	0.50±0.05^*#
sh-Control组	0.18±0.05	0.34±0.07	0.39±0.03	0.57±0.04	0.75±0.07
空白组	0.15±0.03	0.35±0.11	0.42±0.12	0.63±0.10	0.73±0.05
F值	0.873	6.170	5.087	19.958	35.958
P值	0.438	0.011	0.021	<0.001	<0.001

组别	迁移细胞数/ （个/高倍镜视野）	侵袭细胞数/ （个/高倍镜视野）
sh-MUC16组	108.83±7.57	93.50±9.29
sh-Control组	131.33±11.20^*	122.25±7.78^*
空白组	127.83±9.22^*	127.00±9.92^*
F值	9.853	26.034
P值	0.002	<0.001

组别	迁移细胞数/ （个/高倍镜视野）	侵袭细胞数/ （个/高倍镜视野）
sh-MUC16组	108.83±7.57	93.50±9.29
sh-Control组	131.33±11.20^*	122.25±7.78^*
空白组	127.83±9.22^*	127.00±9.92^*
F值	9.853	26.034
P值	0.002	<0.001

组别	MUC16	E-Cad	N-Cad
sh-MUC16组	0.21±0.05	0.65±0.05	0.27±0.12
sh-Control组	0.72±0.11^*	0.23±0.09^*	0.86±0.05^*
空白组	0.76±0.16^*	0.28±0.04^*	0.79±0.04^*
F值	42.627	76.559	105.144
P值	<0.001	<0.001	<0.001