Abstract:

Objective To investigate the effects of antiestrogen（tamoxifen）on the proliferation and the expression level of CC chemokine ligand （CCL）2 of osteosarcoma cells. Methods The difference of estrogen receptor（ER） expression between osteosarcoma cells（143B,U-2OS） and osteoblast（hFOB1.19） was observed. CCK8 experiment and plate colony formation assay were used to observe the effects of tamoxifen on the proliferation of osteosarcoma cells. The relative expression levels of inflammatory factors,IL-6,IL-8,TGF-β,CCL2 and CCL5 in osteosarcoma cells were determined by fluorescence quantitative polymerase chain reaction（PCR）. The effects of tamoxifen on CCL2 expression of osteosarcoma cells in cell culture medium were determined by enzyme-linked immunosorbent assay（ELISA）. Results The expression level of ER mRNA in osteosarcoma cells（143B,U-2OS） was lower than that in osteoblast（hFOB1.19）. Tamoxifen could inhibit the proliferation of osteosarcoma cells. Tamoxifen could inhibit the expressions of inflammatory factors,IL-6,IL-8,TGF-β,CCL2 and CCL5,especially the chemokine CCL2 in osteosarcoma cells. Conclusions Tamoxifen could inhibit the proliferation of osteosarcoma cells and the expression of CCL2,suggesting that it might be used as a adjuvant drug for postoperative chemotherapy of osteosarcoma.

Key words: Estrogen receptor, CC chemokine ligand 2, Tamoxifen, Osteosarcoma