Laboratory Medicine ›› 2020, Vol. 35 ›› Issue (8): 837-842.DOI: 10.3969/j.issn.1673-8640.2020.08.022
Previous Articles Next Articles
HOU Yanyan1, FAN Jinong2, LIU Xiangfan1, NI Peihua1
Received:
2020-03-16
Online:
2020-08-30
Published:
2020-09-24
CLC Number:
HOU Yanyan, FAN Jinong, LIU Xiangfan, NI Peihua. Role of circular RNA in tumor proliferation and metastasis[J]. Laboratory Medicine, 2020, 35(8): 837-842.
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.shjyyx.com/EN/10.3969/j.issn.1673-8640.2020.08.022
方法 | 优点 | 局限性 | 参考文献 |
---|---|---|---|
circRNA测序 | 有助于发现有潜力的circRNA分 子 | 检测结果可能包括对RNase R有抗性的线性 RNA | [9] |
微阵列 | 需要较少的生物信息学知识,是 RNA-seq可行的替代方法 | 只能分析有限数量的已知circRNA,,并且不 能提供对应的线性对应物的信息 | [10] |
Northern印迹分析 | 可用于评估circRNA的大小、同 工型、序列和丰度 | 操作复杂,不能用于高通量检测 | [11] |
实时定量PCR | 实验条件简单,并且可以验证 circRNA的完整序列 | 存在滚环效应影响检测的精确度 | [5] |
数字液滴PCR | 可量化circRNA拷贝数 | 需要专用设备和试剂 | [12] |
NanoString nCounter荧光条 形码标记单分子检测技术 | 有一定的检测通量且能绝对定量 | 需要专用设备和试剂 | [13] |
方法 | 优点 | 局限性 | 参考文献 |
---|---|---|---|
circRNA测序 | 有助于发现有潜力的circRNA分 子 | 检测结果可能包括对RNase R有抗性的线性 RNA | [9] |
微阵列 | 需要较少的生物信息学知识,是 RNA-seq可行的替代方法 | 只能分析有限数量的已知circRNA,,并且不 能提供对应的线性对应物的信息 | [10] |
Northern印迹分析 | 可用于评估circRNA的大小、同 工型、序列和丰度 | 操作复杂,不能用于高通量检测 | [11] |
实时定量PCR | 实验条件简单,并且可以验证 circRNA的完整序列 | 存在滚环效应影响检测的精确度 | [5] |
数字液滴PCR | 可量化circRNA拷贝数 | 需要专用设备和试剂 | [12] |
NanoString nCounter荧光条 形码标记单分子检测技术 | 有一定的检测通量且能绝对定量 | 需要专用设备和试剂 | [13] |
相关蛋白/通路 | circRNA | 失调情况 | 靶标miRNA | 靶标蛋白 | 肿瘤 | 参考文献 |
---|---|---|---|---|---|---|
细胞周期相关蛋白/激酶 | ||||||
cyclin | circNR3C1 | 下调 | miR-27a-3p | cyclin D1 | 膀胱癌 | [14] |
has_circ_0000204 | 下调 | miR-191 | KLF6 | 肝癌 | [15] | |
CKI | circ-ZKSCAN1 | 下调 | miR-1178-3p | P21 | 膀胱癌 | [16] |
circRNA BCRC-3 | 下调 | miR-182-5p | P27 | 膀胱癌 | [17] | |
CDK | circ-Foxo3 | 上调 | - | P21/CDK2 | 乳腺癌 | [18] |
circ-Ccnb1 | 下调 | - | cyclin B1/CDK1 | 乳腺癌 | [19] | |
EMT相关蛋白 | ||||||
EMT-TF | circPRMT5 | 上调 | miR-30c | Snail | 尿道上皮癌 | [20,21] |
circNUP214 | 上调 | miR-145 | ZEB2 | 甲状腺乳头状癌 | [22] | |
circ-10720 | 上调 | miR-490-5p | Twist1 | 肝细胞癌 | [23] | |
cirs-7 | 上调 | miR-7 | KLF4 | 食管鳞状细胞癌 | [24] | |
circNHSL1 | 上调 | miR-1306-3p | Vimentin | 胃癌 | [25] | |
细胞黏附分子与骨架蛋白 | circAMOTL1L | 下调 | miR-193a-5p | Pcdha8 | 前列腺癌 | [25] |
circMTO1 | 上调 | miR-221 | N-CAD | 膀胱癌 | [26] | |
circPTK2 | 下调 | miR-429/miR-200b-3p | TIF1γ | 非小细胞肺癌 | [34] | |
相关信号通路 | ||||||
TGF-β/Smad | circRIP2 | 上调 | miR-1305 | TGF-β2 | 膀胱癌 | [28] |
Wnt/β-catenin | circMYO10 | 上调 | miR-370-3p | RUVBL1 | 骨肉瘤 | [29] |
Notch | circNFIX | 上调 | miR-34a-5p | Notch1 | 神经胶质瘤 | [30] |
PI3K/Akt/mTOR | circNRIP1 | 上调 | miR-149-5p | AKT1 / mTOR | 胃癌 | [31] |
NF-κB | circRNA-000911 | 上调 | miR-449a | Notch1 | 乳腺癌 | [32] |
相关蛋白/通路 | circRNA | 失调情况 | 靶标miRNA | 靶标蛋白 | 肿瘤 | 参考文献 |
---|---|---|---|---|---|---|
细胞周期相关蛋白/激酶 | ||||||
cyclin | circNR3C1 | 下调 | miR-27a-3p | cyclin D1 | 膀胱癌 | [14] |
has_circ_0000204 | 下调 | miR-191 | KLF6 | 肝癌 | [15] | |
CKI | circ-ZKSCAN1 | 下调 | miR-1178-3p | P21 | 膀胱癌 | [16] |
circRNA BCRC-3 | 下调 | miR-182-5p | P27 | 膀胱癌 | [17] | |
CDK | circ-Foxo3 | 上调 | - | P21/CDK2 | 乳腺癌 | [18] |
circ-Ccnb1 | 下调 | - | cyclin B1/CDK1 | 乳腺癌 | [19] | |
EMT相关蛋白 | ||||||
EMT-TF | circPRMT5 | 上调 | miR-30c | Snail | 尿道上皮癌 | [20,21] |
circNUP214 | 上调 | miR-145 | ZEB2 | 甲状腺乳头状癌 | [22] | |
circ-10720 | 上调 | miR-490-5p | Twist1 | 肝细胞癌 | [23] | |
cirs-7 | 上调 | miR-7 | KLF4 | 食管鳞状细胞癌 | [24] | |
circNHSL1 | 上调 | miR-1306-3p | Vimentin | 胃癌 | [25] | |
细胞黏附分子与骨架蛋白 | circAMOTL1L | 下调 | miR-193a-5p | Pcdha8 | 前列腺癌 | [25] |
circMTO1 | 上调 | miR-221 | N-CAD | 膀胱癌 | [26] | |
circPTK2 | 下调 | miR-429/miR-200b-3p | TIF1γ | 非小细胞肺癌 | [34] | |
相关信号通路 | ||||||
TGF-β/Smad | circRIP2 | 上调 | miR-1305 | TGF-β2 | 膀胱癌 | [28] |
Wnt/β-catenin | circMYO10 | 上调 | miR-370-3p | RUVBL1 | 骨肉瘤 | [29] |
Notch | circNFIX | 上调 | miR-34a-5p | Notch1 | 神经胶质瘤 | [30] |
PI3K/Akt/mTOR | circNRIP1 | 上调 | miR-149-5p | AKT1 / mTOR | 胃癌 | [31] |
NF-κB | circRNA-000911 | 上调 | miR-449a | Notch1 | 乳腺癌 | [32] |
[1] | PATOP I L,WÜST S,KADENER S. Past,present,and future of circRNAs[J]. EMBO J,2019,38(16):e100836. |
[2] | SALZMAN J,GAWAD C,WANG P L,et al. Circular RNAs are the predominant transcript isoform from hundreds of human genes in diverse cell types[J]. PLoS One,2012,7(2):e30733. |
[3] | JECK W R,SORRENTINO J A,WANG K,et al. Circular RNAs are abundant,conserved,and associated with ALU repeats[J]. RNA,2013,19(2):141-157. |
[4] | DONG R,MA X K,CHEN L L,et al. Increased complexity of circRNA expression during species evolution[J]. RNA Biol,2017,14(8):1064-1074. |
[5] | WEI C Y,ZHU M X,LU N H,et al. Circular RNA circ_0020710 drives tumor progression and immune evasion by regulating the miR-370-3p/CXCL12 axis in melanoma[J]. Mol Cancer,2020,19(1):84. |
[6] | RYBAK-WOLF A,STOTTMEISTER C,GLAŽAR P,et al. Circular RNAs in the mammalian brain are highly abundant,conserved,and dynamically expressed[J]. Mol Cell,2015,58(5):870-885. |
[7] | ZHU Y J,ZHENG B,LUO G J,et al. Circular RNAs negatively regulate cancer stem cells by physically binding FMRP against CCAR1 complex in hepatocellular carcinoma[J]. Theranostics,2019,9(12):3526-3540. |
[8] | CHEN L,KONG R,WU C,et al. Circ-MALAT1 functions as both an mRNA translation brake and a microRNA sponge to promote self-renewal of hepatocellular cancer stem cells[J]. Adv Sci(Weinh),2019,7(4):1900949. |
[9] | WANG S,TANG D,WANG W,et al. circLMTK2 acts as a sponge of miR-150-5p and promotes proliferation and metastasis in gastric cancer[J]. Mol Cancer,2019,18(1):162. |
[10] | XU G,YE D,ZHAO Q,et al. circNFIC suppresses breast cancer progression by sponging miR-658[J]. J Cancer,2020,11(14):4222-4229. |
[11] | DING L,ZHAO Y,DANG S,et al. Circular RNA circ-DONSON facilitates gastric cancer growth and invasion via NURF complex dependent activation of transcription factor SOX4[J]. Mol Cancer,2019,18(1):45. |
[12] | LI T,SHAO Y,FU L,et al. Plasma circular RNA profiling of patients with gastric cancer and their droplet digital RT-PCR detection[J]. J Mol Med,2018,96(1):85-96. |
[13] | DAHL M,DAUGAARD I,ANDERSEN M S,et al. Enzyme-free digital counting of endogenous circular RNA molecules in B-cell malignancies[J]. Lab Invest,2018,98(12):1657-1669. |
[14] | ZHENG F,WANG M,LI Y,et al. CircNR3C1 inhibits proliferation of bladder cancer cells by sponging miR-27a-3p and downregulating cyclin D1 expression[J]. Cancer Lett,2019,460:139-151. |
[15] | TIAN F,YU C,WU M,et al. MicroRNA-191 promotes hepatocellular carcinoma cell proliferation by has_circ_0000204/miR-191/KLF6 axis[J]. Cell Prolif,2019,52(5):e12635. |
[16] | BI J,LIU H,DONG W,et al. Circular RNA circ-ZKSCAN1 inhibits bladder cancer progression through miR-1178-3p/p21 axis and acts as a prognostic factor of recurrence[J]. Mol Cancer,2019,18(1):133. |
[17] | XIE F,LI Y,WANG M,et al. Circular RNA BCRC-3 suppresses bladder cancer proliferation through miR-182-5p/p27 axis[J]. Mol Cancer,2018,17(1):144. |
[18] | DU W W,YANG W,LIU E,et al. Foxo3 circular RNA retards cell cycle progression via forming ternary complexes with p21 and CDK2[J]. Nucleic Acids Res,2016,44(6):2846-2858. |
[19] | FANG L,DU W W,AWAN F M,et al. The circular RNA circ-Ccnb1 dissociates Ccnb1/Cdk1 complex suppressing cell invasion and tumorigenesis[J]. Cancer Lett,2019,459:216-226. |
[20] | CHEN X,CHEN R X,WEI W S,et al. PRMT5 circular RNA promotes metastasis of urothelial carcinoma of the bladder through sponging miR-30c to induce epithelial-mesenchymal transition[J]. Clin Cancer Res,2018,24(24):6319-6330. |
[21] | MA H B,YAO Y N,YU J J,et al. Extensive profiling of circular RNAs and the potential regulatory role of circRNA-000284 in cell proliferation and invasion of cervical cancer via sponging miR-506[J]. Am J Transl Res,2018,10(2):592-604. |
[22] | LI X,TIAN Y,HU Y,et al. CircNUP214 sponges miR-145 to promote the expression of ZEB2 in thyroid cancer cells[J]. Biochem Biophys Res Commun,2018,507(1-4):168-172. |
[23] | MENG J,CHEN S,HAN J X,et al. Twist1 regulates vimentin through Cul2 circular RNA to promote EMT in hepatocellular carcinoma[J]. Cancer Res,2018,78(15):4150-4162. |
[24] | HUANG H,WEI L,QIN T,et al. Circular RNA ciRS-7 triggers the migration and invasion of esophageal squamous cell carcinoma via miR-7/KLF4 and NF-κB signals[J]. Cancer Biol Ther,2019,20(1):73-80. |
[25] | ZHU Z,RONG Z,LUO Z,et al. Circular RNA circNHSL1 promotes gastric cancer progression through the miR-1306-3p/SIX1/vimentin axis[J]. Mol Cancer,2019,18(1):126. |
[26] | YANG Z,QU C B,ZHANG Y,et al. Dysregulation of p53-RBM25-mediated circAMOTL1L biogenesis contributes to prostate cancer progression through the circAMOTL1L-miR-193a-5p-Pcdha pathway[J]. Oncogene,2019,38(14):2516-2532. |
[27] | LI Y,WAN B,LIU L,et al. Circular RNA circMTO1 suppresses bladder cancer metastasis by sponging miR-221 and inhibiting epithelial-to-mesenchymal transition[J]. Biochem Biophys Res Commun,2019,508(4):991-996. |
[28] | SU Y,FENG W,SHI J,et al. circRIP2 accelerates bladder cancer progression via miR-1305/Tgf-β2/smad3 pathway[J]. Mol Cancer,2020,19(1):23. |
[29] | CHEN J,LIU G,WU Y,et al. CircMYO10 promotes osteosarcoma progression by regulating miR-370-3p/RUVBL1 axis to enhance the transcriptional activity of β-catenin/LEF1 complex via effects on chromatin remodeling[J]. Mol Cancer,2019,18(1):150. |
[30] | XU H,ZHANG Y,QI L,et al. NFIX circular RNA promotes glioma progression by regulating miR-34a-5p via Notch signaling pathway[J]. Front Mol Neurosci,2018,11:225. |
[31] | ZHANG X,WANG S,WANG H,et al. Circular RNA circNRIP1 acts as a microRNA-149-5p sponge to promote gastric cancer progression via the AKT1/mTOR pathway[J]. Mol Cancer,2019,18(1):20. |
[32] | WANG H,XIAO Y,WU L,et al. Comprehensive circular RNA profiling reveals the regulatory role of the circRNA-000911/miR-449a pathway in breast carcinogenesis[J]. Int J Oncol,2018,52(3):743-754. |
[33] | WANG W,WANG J,ZHANG X,et al. Serum circSETDB1 is a promising biomarker for predicting response to platinum-taxane-combined chemotherapy and relapse in high-grade serous ovarian cancer[J]. Onco Targets Ther,2019,12:7451-7457. |
[34] | GAN X,ZHU H,JIANG X,et al. CircMUC16 promotes autophagy of epithelial ovarian cancer via interaction with ATG13 and miR-199a[J]. Mol Cancer,2020,19(1):45. |
[35] | HE F,ZHONG X,LIN Z,et al. Plasma exo-hsa_circRNA_0056616:a potential biomarker for lymph node metastasis in lung adenocarcinoma[J]. J Cancer,2020,11(14):4037-4046. |
[36] | LIU X,TANG H,LIU J,et al. hsa_circRNA_101237:a novel diagnostic and prognostic biomarker and potential therapeutic target for multiple myeloma[J]. Cancer Manag Res,2020,12:2109-2118. |
[1] | ZHOU Yunlan, SHEN Lisong. Clinical application and challenges of liquid biopsy biomarkers in non-small cell lung cancer [J]. Laboratory Medicine, 2023, 38(9): 807-811. |
[2] | PENG Wei, LI Yungai, XU Jing, LIU Hua, YANG Cuixia, SHEN Yunyue. Serum inflammatory factors combined with PSA and f-PSA in the auxiliary diagnosis of prostate cancer [J]. Laboratory Medicine, 2023, 38(9): 849-854. |
[3] | DING Xiaoyuan, DAI Jinsheng, JIAO Ronghong, MA Suli, ZHU Haifeng, WU Mengya, CHE Yanran, ZHANG Lei. Influence of obesity/overweight on inflammatory factors of Th1/Th2 and Th17/Treg in children with asthma attacks [J]. Laboratory Medicine, 2023, 38(8): 748-752. |
[4] | ZHAO Jing, WANG Pei, LU Dandan, QI Dandan, LI Xuan. Expression levels of serum miR-4443 and TRAF4 in patients with hemorrhagic stroke [J]. Laboratory Medicine, 2023, 38(5): 413-418. |
[5] | GAO Feng. Clinical application of novel tumor biomarkers:prospects and challenges [J]. Laboratory Medicine, 2023, 38(4): 303-306. |
[6] | SONG Shiwei, LI Jingwu, LIN Tao, LIU Zeyu, WANG Zhiqiang, SUN Weidong. Relationship between GPNMB and non-small cell lung cancer immune infiltration and prognosis [J]. Laboratory Medicine, 2023, 38(4): 347-351. |
[7] | WU Jiong, HU Jiahua, SHI Meifang, LIU Tao, DAI Jie, LU Xinyi, ZOU Zheng. Research progress of biomarkers of prostate cancer [J]. Laboratory Medicine, 2023, 38(2): 190-195. |
[8] | QIAN Linyu, LIANG Weifang, TANG Sichen. Determination and clinical significance of NLRP3,CTRP6 and IL-1β in patients with gestational diabetes mellitus [J]. Laboratory Medicine, 2023, 38(10): 936-940. |
[9] | SHANG Zhenjun, WEI Wei, LIU Ruihan. Effect of inhibiting PTBP1 on the proliferation and invasiveness of choroidal melanoma cells [J]. Laboratory Medicine, 2023, 38(1): 51-55. |
[10] | WU Yating, LI Zhuolin, LEI Yan, JIA Ruxue, ZHANG Shenghang, WANG Shuiliang. Research progress of miRNA in urine as a biomarker for common malignant tumors [J]. Laboratory Medicine, 2023, 38(1): 94-99. |
[11] | KONG Yujie, WANG Chen, HE Bing. Research progress of N6-methyladenosine in biological function and detection technology [J]. Laboratory Medicine, 2022, 37(9): 872-876. |
[12] | WANG Rui, LI Zhaoyan, ZHAO Aiguang. Application of circulating tumor DNA detection in the diagnosis and treatment of gastric cancer [J]. Laboratory Medicine, 2022, 37(9): 877-881. |
[13] | LIU Xingqiang, NING Lifen, LI Lin, CHEN Zhongcheng. Correlation of lung cancer clinicopathological characteristics with FR+-CTC,ANXA2 and ProGRP [J]. Laboratory Medicine, 2022, 37(8): 735-740. |
[14] | WEN Shuzhan, FU Zile, CHEN Shuying. Expression and bioinformatics analysis of hsa-miR-34a in tumors [J]. Laboratory Medicine, 2022, 37(7): 657-663. |
[15] | GAO Feng. Research progress of new tumor molecular markers in the age of precision medicine:from accurate diagnosis to precision chemotherapy [J]. Laboratory Medicine, 2022, 37(4): 309-312. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||