Abstract: Objective　To establish a time-resolved fluoroimmunoassay（TRFIA）for anti-thyroglobulin antibody（TgAb）.   Methods　The thyroglobulin antigen was coated on the 96 well plates. A goat anti-mouse IgG-europium (Eu^3＋) was used to the detection. Serum TgAb level was detected by TRFIA.  Results　The sensitivity was 1.0 IU/mL. The within-run coefficient of variation (CV)was 3.3%- 4.1%. The between-run CV was 3.7%- 4.7%. The average recovery was 97.9%. The kit was stable. The correlation coefficient with TgAb by electrochemiluminescence immunoassay (ECLIA) was 0.881 3. The TgAb levels between males and females had no statistical significance. The normal range of TgAb was ≤110 IU/mL.  Conclusions　The established TRFIA for the detection of TgAb in serum is a sensitive and reliable assay, which would be useful for the clinical diagnosis of thyroid diseases.

Key words: Time-resolved fluoroimmunoassay, Anti-thyroglobulin antibody, Thyroid disease