Table of Content

30 November 2012, Volume 27 Issue 11

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Evaluation of electrochemical luminescence assay in serum herpes simplex virus type Ⅱ IgG antibody detection．

CHEN Daihong，MAO Huijun，QIU Jianping，WU Wenjuan

2012, 27(11):  887-889. 

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Objective　To compare the results of anti-herpes simplex virus type 2（HSV-2）IgG antibody detected by electro-chemiluminescence assay（ECLIA）and enzyme-linked immunosorbent assay（ELISA），and evaluate the performance and application significance of ECLIA. Methods　Anti-HSV-2 specific IgG antibody of 400 serum samples were detected by ECLIA（Roche）and ELISA（Trinity，Ireland）. The samples with inconsistent results were detected by anti-HSV-2 IgG antibody detection kit of Euroimmun Biotechnology Co.Ltd.. Results　The positive rates were 39.0% by ELISA and 37.8% by ECLIA respectively. Compared with ELISA，the false negative rate of ECLIA was 1.3%，and the false positive rate was 0.8%. The relative sensitivity and relative specificity were 96.8% and 98.8%. The coincidence rate of the 2 methods was 98.0%. The 13 samples with inconsistent results（including 2 uncertain results）were detected by anti-HSV-2 IgG antibody detection kit of Euroimmun Biotechnology Co.，Ltd.. The results indicated that 6 samples were consistent with ECLIA，and 5 samples were consistent with ELISA. Conclusions　ECLIA and ELISA results had good coincidence，and Roche ECLIA can be used as a fast，accurate，sensitive，high-specific reagent for clinical application in the determination of anti-HSV-2 IgG antibody.

Detection of tumor necrosis factor and sphingomyelinase in the patients with rheumatoid arthritis and its clinical significance

SU Min 1，SHOU Tao 2，SUN Hong 1

2012, 27(11):  890-892. 

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Objective　To investigate the clinical significance of sphingomyelinase（sMase）and tumor necrosis factor-alpha（TNF-α）in patients with rheumatoid arthritis（RA）and atherosclerosis（AS）. Methods　The cultured human umbilical cord vein vascular endothelial cells（EC）were stimulated by TNF-α， and the activity of sMase in the supernatant was detected. The serum sMase activity and TNF-α levels were detected in 124 RA patients [66 patients with only RA and 58 RA patients also with coronary heart disease（CHD）] and the corresponding control group. Results　The sMase activity of EC increased significantly after the stimulation of TNF-α. The serum sMase activity of RA group was higher than that of control group，especially the activity of RA combined CHD group was the highest. The serum TNF-α levels of only RA group and RA combined CHD group were higher than that of control group. Conclusions　The sMase activity of EC increases with the stimulation of TNF-α. The increases of TNF-α level and sMase activity in RA patients are related to vascular endothelial damage. Therefore，sMase activity may become one of the laboratory diagnostic indicators of vascular endothelial cell dysfunction（ECD）.

Analysis of serum antibody to Hepatitis C Virus in 31551 patients of Fuzhou area

CHEN Yabin，YI Xunfei，LAN Xiaopeng

2012, 27(11):  893-895. 

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Objective　To analyze the determination results of antibody to hepatitis C virus（HCV）in patients and healthy subjects， understand the infection state of hepatitis C in Fuzhou， and to provide reference for clinical treatment. Methods　The antibodies to HCV in 31 551 patients and healthy subjects were determined by chemiluminescence immunoassay. Results　In all 31 551 patients and healthy subjects，the total positive rate for antibody to HCV was 1.15%（363/31 551）.The positive rate of males was 1.33％，and the positive rate of females was 0.97％.The highest in all age groups was the 61-70 years old group， which the positive rate was 1.83%.In the all antibody to HCV positive patients，patients with the S/CO ratio of 1.01-5.00 and >20.01 accounted for 47.38% and 33.88%，respectively.Among 319 positive patients with retrospective clinical data，patients with benign liver diseases，other benign diseases， liver cancer and other cancers accounted for 7.21%，83.39%，6.27% and 16.93%，respectively. Patients with the history of blood transfusion，surgery，hemodialysis，hepatitis B virus infection，drug abuse and mother-to-child transmission accounted for 4.39%，34.80%，18.18%，19.44%，0.63% and 0.63%，respectively. Conclusions　The situation of HCV infection is complicated.Besides blood transmission，other routes of transmission cannot be eglected.Subjects with no clinical symptom must be routinely detected for the antibody to HCV.

Methodology evaluation of particle-enhanced turbidimetric immunoassay for determining alpha-fetoprotein

ZENG Fangyin 1，ZHANG Yuming 2，ZHANG Peng 1，LIN Li 3，SUN Dehua 1

2012, 27(11):  896-899. 

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Objective　To evaluate methodologically particle-enhanced turbidimetric immunoassay（PETIA）for determining quantitatively alpha-fetoprotein（AFP）. Methods　AFP was determined quantitatively by OLYMPUS AU5400 automatic biochemical analyzer， and the precision， reagent stability， detection low limit， sensitivity， linear range， interference and accuracy of this method were investigated. The correlation of fresh serum sample results between SIEMENS CENTAUR electro-chemiluminescence assay（ECLIA）and PETIA was analyzed. The reference interval was verified. Results　Testing 29.95 and 132.25 ng/mL patient samples， the within-run coefficients of variation（CV）were 1.67% and 1.52％，and the day-to-day CV were 7.17% and 7.84% respectively. The detection low limit and sensitivity were 0.52 and 0.53 ng/mL respectively. The linear correlation coefficient（r²）was 0.999 4 from 5.4 to 256 ng/mL， and the regression equation was Y=1.014 X +1.063 3. Both of the relative deviations of the high-level serum（5.96%）and low-level serum（8.40%）were lower than the evaluation criteria "target value±20%" of proficiency testing（PT） from National Center for Clinical Laboratories. There was a good correlation between PETIA with SIEMENS electro-chemiluminescence analyzer in 107 patients，Y_ECLIA=1.138 7X_PETIA+2.835 3（r²=0.992 1）. There was no obvious interference from hemolysis， jaundice and lipidemia. All the results of 20 healthy subject samples were included in the reference interval recommended by the manufacture. Conclusions　PETIA is a convenient， rapid， cheap， accurate and reliable method for determining AFP， particularly be suitable for large-scale health screening physical examination.

Application of CLSI EP12-A2 document in the performance evaluation of qualitative test of HBeAb

HUANG Wujiao1，HUANG Xianzhang1，ZHUANG Junhua1，LI Qiang1，HUANG Hui1，ZHANG Qiang2

2012, 27(11):  900-903. 

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Objective　To evaluate A and B ELISA kits for detecting qualitatively anti-hepatitis B e antigen antibody（HBeAb），and to provide useful reference for choosing suitable HBeAb kits. Methods　According to Clinical and Laboratory Standards Institute（CLSI） EP12-A2 document，the bias and imprecision of A and B HBeAb kits were analyzed，and their C50，C5-C95 intervals and imprecision curves were compared.The HBeAb of 910 specimens were simultaneously determined by both A and B kits.The 95% confidence interval（CI）for concordance was calculated.The Kappa value was calculated to evaluate their concordance. Results　C50 for A kit was less than that for B kit.C5-C95 interval of A kit was narrower than that of B kit.The imprecision curve of A kit was steeper than that of B kit.The 95%CI for concordance was calculated as 93.2%-96.1%，and the Kappa value was 0.87. Conclusions　EP12-A2 is a useful protocol for the evaluation of qualitative test performance.

Investigation on the reference range of venous whole blood cell counts in healthy elder people

SHENYan，XUBingxin，WANGJianbiao

2012, 27(11):  904-907. 

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Objective　To investigate a reference range of blood cell counts in healthy elder people. Methods　An automated hematological analyzer（Sysmex XT-1800） was used to determine the parameters of venous whole blood cell counts，including white blood cell（WBC），red blood cell（RBC），hemoglobin（Hb），hematocrit（HCT），mean corpuscular volume（MCV），mean corpuscular hemoglobin（MCH），mean corpuscular hemoglobin concentration（MCHC），red cell distribution width（RDW），platelet（PLT） and mean platelet volume（MPV）. A total of 395 healthy elder people（197 males and 198 females ≥65 years old） and 188 controls < 65 years old were enrolled. Results　The WBC，RBC，Hb，HCT，MCH and MCHC in elder males were higher than those in elder females（P＜0.05，P＜0.001）. PLT in elder males was lower than that in elder females（P＜0.001）. The MCV，RDW and MPV had no statistical significance between males and females（P＞0.05）. The reference ranges of WBC were（4.10-9.81）×10⁹/L in males and（4.14-8.94）×10⁹/L in females，RBC:（3.68-5.13）×10¹²/L in males and（3.52-5.12）×10⁹/L in females，Hb: 119-161 g/L in males and 113-147 g/L in females，HCT: 34.0%-46.0% in males and 33.0%-47.0% in females，MCV: 84.0-99.3 fL，MCH: 29.1-34.7 pg in males and 27.7-33.7 pg in females，MCHC: 334-366 g/L in males and 328-360 g/L in females，RDW: 12.0%-14.9%，PLT:（102-257）×10⁹/L in males and（123-337）×10⁹/L in females and MPV: 7.7-12.1 fL. The reference ranges of RBC，Hb，HCT，MCH，MCHC，PLT and MPV in elder males had statistical significance with the males in the control group（P＜0.05）. In elder females，the reference ranges of MCV，MCH，MCHC and MPV had statistical significance with the females in the control group（P＜0.05）. The age of elder people had the positive correlation with WBC，MCV and RDW（r＝0.106，0.208 and 0.115，P＜0.05），had the negative correlation with RBC，Hb，HCT and MCHC（r＝－0.207，－0.200，－0.139 and －0.238，P＜0.001），and had no correlation with MCH，PLT and MPV. Conclusions　The WBC in the elder people is higher than that in the controls. Excluding RDW with positive correlation，the other parameters have negative correlations with age increasing. PLT in elder males decreases，and MPV increases. They are different with the current reference ranges. It is important to establish the reference range of venous whole blood cell counts in healthy elder people.

Contrast observation of ITS-PCR identification and phenotypic identification for nontuberculous Mycobacterium

GUI Jing，WANG Feng，LI Jinli

2012, 27(11):  908-912. 

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Objective　To evaluate the clinical significance of 16S-23S rRNA intergenic transcribed spacer-polymerase chain reaction（ITS-PCR）identification for nontuberculous Mycobacterium（NTM）. Methods　A total of 80 NTM identified by ITS-PCR were randomly selected. Phenotypic characteristics of NTM were identified by growth characterization test and drug sensitivity test.The results for the 3 identification methods were analyzed contrastively. Results　The proportion of Mycobacterium avium complex（MAC） and Mycobacterium abscessus was 85%.Mycobacterium abscessus，Mycobacterium kansasii，Mycobacterium fortuitum and Mycobacterium szulgai were identified accurately by ITS-PCR.The results were consistent among the results of growth characterization test，drug sensitivity test and ITS-PCR except that of MAC. Conclusions　The combination of ITS-PCR with the phenotypic identification for NTM is suitable to the clinical application.

Laboratory diagnosis in food-borne diseases caused by Salmonella and homology analysis in Salmonella typhimurium

SUN Kangde 1，LI Jieqiong 1，CHEN Fuxiang 1， CHEN Jun 2，WANG Yu 2

2012, 27(11):  913-916. 

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 Objective　To increase the detection rate of Salmonella in intestinal tract by the improved isolation and identification method，and to fundamentally understand the major Salmonella and its molecular typing in Shanghai，establish the database of pulsed-field gel electrophoresis（PFGE）typing for rapid source-tracing and control dissemination of the disease during Salmonella outbreaks. Methods　The improved isolation and identification method was used to detect suspected Salmonella in fecal specimens. PFGE was used to perform molecular typing of Salmonella typhimurium. Results　Salmonella enteritidis was main among the isolates of Salmonella by the improved isolation and identification method，accounting for 42.1%.The second was Salmonella typhimurium，accounting for 13.2%.Two distinct DNA fragment profiles，PFGE type A and B，were observed，including type A: 6 isolates and type B: 4 isolates respectively.Type A could be subclassified further into 4 subtypes. Conclusions　The improved isolation and identification method could effectively increase the detection rate of Salmonella in fecal specimens.PFGE is an effective method to study the molecular typing.The research shows relatively close kinship in molecular typing of Salmonella typhimurium.

Comparative study on genotyping of Candida tropicalis by repetitive sequence-based PCR and multilocus sequence typing

JIANG Cen，DONG Danfeng，YU Beiqin，PENG Yibing

2012, 27(11):  917-920. 

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Objective　To compare repetitive sequence-based polymerase chain reaction（REP-PCR）and multilocus sequence typing（MLST）in genotyping of Candida tropicalis. Methods　REP-PCR was performed on 147 clinical isolates of Candida tropicalis collected from 6 hospitals of 5 provinces.Primer Ca-21，Ca-22 and Com-21 were used pairly to find the most suitable pair.Three isolates of Candida tropicalis from different REP-PCR types were tested by MLST.Six loci in housekeeping genes were sequenced after amplification，which were compared with the MLST database to obtain sequence type（ST）. Results　Eight REP-PCR types were found in 147 isolates of Candida tropicalis with primer Com21-Com21，which had the best genotyping effect.Type A-H were corresponding with ST146，NEW1，ST136，ST127，ST177，ST169，NEW2 and ST117 by MLST respectively. Conclusions　REP-PCR offers a simple and rapid method for molecular typing，which has a similar discriminatory power with MLST.Therefore，REP-PCR can be the first choice in laboratory，especially for a large number of isolates.

Establishment of hepatitis B virus X gene eukaryotic expression vector

LI Xiaofei

2012, 27(11):  921-924. 

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Objective　To establish hepatitis B virus（HBV）X gene eukaryotic expression vector and identify its expression. Methods　Polymerase chain reaction（PCR）was used to amplify X gene sequence，and the pcDNA6（＋）vector and X gene PCR product double enzyme digestion transformed into Escherichia coli DH5 alpha.The positive clones were screened，and the double enzyme digestion and DNA sequencing were used to establish HBV X gene eukaryotic expression vector pcDNA6（＋）-HBx.By polyetherimide（PEI）method，the transformation into HepG2 cells was performed，and Western blot identification of target protein was carried out. Results　After pcDNA6-HBx enzyme digestion，agarose gel electrophoresis found HBx gene fragment，and sequencing results showed containing the complete X gene fragment.Western blot results showed that the pcDNA6（＋）-HBx in HepG2 cells expressed X protein. Conclusions　The eukaryotic expression vector pcDNA6（＋）-HBx is established successfully，and there is X protein expression in HepG2 cells. It provides convenience for the further study of HBV X gene with chronic hepatitis and hepatocellular carcinoma.

Simultaneous determination of the concentrations of fluoxetine and quetiapine in human plasma by HPLC

ZHANG Yuechun，LING Weiming，QIU Yawen

2012, 27(11):  925-928. 

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Objective　To establish a method for determining the concentrations of fluoxetine and quetiapine simultaneously in human plasma by high performance liquid chromatography（HPLC）. Methods　The plasma concentrations of fluoxetine and quetiapine were determined by HPLC.The column was Inertsil ODS-C18 column（4.6 mm×150 mm，5 μm），the mobile phase was 0.05 mol/L ammonium acetate-methanol（volume ratio: 22∶78），the flow rate was 0.8 mL/min， the detection wavelength was 254 nm，the column temperature was kept at 40℃，the extractant was ethylacetate-dichloromethane（volume ratio: 77∶23），and the internal standard was carbamazepine. Results　Chromatographic peaks of carbamazepine，quetiapine and fluoxetine were completely separated，and all peak shapes were good.The retention times were 4.91，6.53 and 7.48 min，respectively，and the interference of plasma impurities on the determination did not occur.The calibration curves showed good linearity in range of 10-480 μg/L for fluoxetine and 20-1 600 μg/L for quetiapine（r=0.990 5 and 0.992 9）.When the signal-to-noise ratio（S/N）was 3，the detection limits were 5 and 8 μg/L，respectively.The absolute recoveries of fluoxetine and quetiapine in low，middle and high concentrations were >75％，and the relative recoveries were >95%.The relative standard deviation（RSD） of intra-day and inter-day were <10％. Conclusions　The method is sensitive，accurate and low-cost，and it can be used for clinical drug monitoring and pharmacokinetics studies of fluoxetine and quetiapine.

The determination and clinical application of free light chain in children with viral encephalitis

AI Hongwu1，QIU Yan1，ZHANG Jiayun1，CHEN Sha1，LIU Zhisheng2

2012, 27(11):  929-931. 

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Objective　To investigate the clinical application significance of free light chain determination in children with viral encephalitis. Methods　Albumin（Alb），immunoglobulin G（IgG）and free light chain were determined in cerebrospinal fluids（CSF） and sera of 24 viral encephalitis children and 12 children without viral encephalitis by BN-II specific protein analyzer. The oligoclonal IgG bands were detected by immunofixation electrophoresis.The Alb CSF/serum quotient（QAlb），IgG CSF/serum quotient（QIgG），kappa CSF/serum quotient（Qκ）and lamda CSF/serum quotient（Qλ）were calculated and analyzed comparatively. Results　The oligoclonal IgG bands were all negative in all children. QAlb，QIgG，Qκ and Qλ in children with viral encephalitis were significantly higher than those in children without viral encephalitis（P<0.01）.QIgG，Qκ and Qλwere significantly higher in blood-brain barrier damage group than in normal group（P<0.01）.In blood-brain barrier damage group，the areas under the receiver operating characteristic curve were 0.884±0.056 for Qκ and 0.878±0.056 for Qλ. Conclusions　Free light chain has application significance for the clinical diagnosis of viral encephalitis in children，and Qκ and Qλ are good indices for the detection of blood-brain barrier damage.

The clinical significance of serum remnant lipoprotein cholesterol determination in dialysis patients with end-stage renal failure

TANG Yurong，WANG Jiliang

2012, 27(11):  932-935. 

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Objective　To detect the concentration of serum remnant lipoprotein cholesterol（RLP-C）in continuous ambulatory peritoneal dialysis（CAPD）and maintenance hemodialysis （MHD）patients，and investigate its clinical significance. Methods　Immunomagnetic separation method was used to measure the serum RLP-C concentrations in 30 healthy subjects and 29 patients with CAPD，28 patients with MHD before dialysis，dialysis for 1 month，6 months and 12 months.The levels of triglyceride（TG），total cholesterol（TC），high density lipoprotein-cholesterol （HDL-C）and low density lipoprotein-cholesterol（LDL-C）were determined.The levels of lipoprotein（a）[Lp（a）]，apolipoprotein A1（apo A1）and apolipoprotein B（apo B）were determined，and the very low density lipoprotein-cholesterol（VLDL-C）was calculated according to the formula: VLDL-C=TC－（HDL-C+LDL-C）.The correlation of RLP-C concentration with hemodialysis for 12 months and other lipid indices was analyzed.  Results　The serum concentrations of RLP-C in CAPD patients before dialysis，dialysis for 1 month，6 months and 12 months were0.26±0.12，0.33±0.14，0.41±0.15 and （0.49 ±0.14）mmol/L，and the serum concentrations of RLP-C in MHD patients were 0.22±0.15，0.31±0.14，0.40±0.16 and（0.49±0.17）mmol/L，which were significantly higher than those in controls [（0.19±0.12）mmol/L]（P<0.05）.The concentration of RLP-C with TG and VLDL-C had positive correlation for dialysis 12 months（r=0.72 and 0.64，P<0.01），and the concentration with TC，LDL-C，HDL-C，apo A1，apo B and Lp（a）had no significant correlation（r=0.14，0.20，－0.20，－0.21，0.21 and 0.18，P>0.05）. Conclusions　RLP-C can be used as a dialysis reference indicator for the prevention and treatment of cardiovascular and cerebrovascular complications.

Analysis on homeostasis model assessment of serum iron and ferritin levels in patients with type 2 diabetes mellitus

WANG Yuerong，ZHANG Dongliang，LI Lan，CAI Feng

2012, 27(11):  936-939. 

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Objective　To investigate the correlation of type 2 diabetes mellitus（T2DM）with homeostasis model assessment（HOMA），serum iron（Fe） and ferritin（Ferr）levels. Methods　A total of 79 patients with T2DM and 50 healthy subjects（control group）were enrolled，and the levels of Fe and fasting insulin（FINS），C-peptide（C-p），glycated albumin（GA），Ferr，fasting plasma glucose（FPG），glycosylated hemoglobin（HbA1C），homeostasis model assessment-insulin resistance（HOMA-IR）index and homeostasis model assessment-pancreatic islet beta cell function（HOMA- β） index were determined and analyzed statistically.The Spearman correlation analysis was performed. Results　Fe and Ferr levels in males were significantly higher than those in females in the control group（P<0.05）.The FINS，GA，Ferr，FPG，HbA1C and HOMA-IR levels in the T2DM group were significantly higher than those in the control group，and HOMA- β indices were significantly lower than those in the control group （P<0.05）.The level of Ferr was positively correlated to GA，FPG，HbA1C and HOMA-IR[correlation coefficients（r）were 0.328，0.333，0.315（P<0.01）and 0.196（P＜0.05）].The level of Ferr was negatively correlated with HOMA-β indices（r=－0.246，P<0.01）.The level of Fe had no significant correlation with HOMA-IR and HOMA- β indices（r=－0.049 and 0.039，P>0.05）. Conclusions　When the Ferr level increases，the insulin resistance degree increases，and the sensitivity of beta cell insulin secretion decreases in the patients with T2DM.

Change and significance on urinary NGAL and serum Cys C in paraquat poisoning patients with early kidney injury

ZHANG Qunmei1，GUO Qinghe2，LU Guangjian1，SONG Zhishan2

2012, 27(11):  940-943. 

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Objective　To investigate the change and significance on urinary neutrophil gelatinase-associated lipocalin（NGAL）and serum cystatin C（Cys C）in paraquat poisoning patients with early acute kidney injury（AKI）. Methods　The blood and urine specimens from 85 patients with paraquat poisoning were collected immediately after admission（≤15 min），2，4，6，8，10，12，18，24，36，48，72 h and 5，7 d.According to the occurrence of AKI，they were classified into AKI group（62 cases）and non-AKI group（23 cases）.A total of 20 healthy subjects were enrolled as control group.The urinary NGAL level was determined by enzyme-linked immunosorbent assay（ELISA）. The serum creatinine（SCr）levels were determined by sarcosine oxidase method. The serum Cys C levels were determined by immuno turbidimetry. The admission immediate detection values were as the baseline values.  Results　In 85 patients with paraquat poisoning，62 patients had AKI（72.94%）. In AKI group at each time point since the beginning of 18h，the SCr levels increased significantly，which were significantly higher than the baseline values and those in the control group（P<0.01）.In AKI group，24，36，48，72 h and 5，7 d SCr levels were significantly higher than those in non-AKI group（P<0.01）.In non-AKI group at each time point，SCr levels had no statistical significance with those in the control group（P>0.05）.Serum Cys C levels in AKI group at 6h were significantly higher than the baseline values（P<0.01）.After the time point，the levels increased gradually and were higher than those in the control group and the baseline values（P<0.01）.In non-AKI group，serum Cys C levels since the beginning of 10h increased significantly，and had a significant difference compared with those in the normal control group and the baseline values（P<0.01）. In AKI group，NGAL levels at 2h were significantly higher than the baseline values，and had gradual rising trend after that time point. Compared with those in the control group and the baseline values，the differences were statistically significant（P<0.01）.In non-AKI group，NGAL levels at 10h began gradually increased，and compared with those in the control group and the baseline values，the difference was statistically significant（P<0.01）.In AKI group，NGAL levels at each time point were obviously higher than those in non-AKI group（P<0.01）. Conclusions　With paraquat poisoning after 2 h，urinary NGAL level is markedly elevated，and serum Cys C in 8h begins to increase.The diagnosis time of AKI is shorter than that of SCr. Urinary NGAL and serum Cys C can be markers for the early diagnosis of AKI after paraquat poisoning.

The roles of serum ox-LDL/β2-GPI complex in patients with type 2 diabetes mellitus complicated with coronary heart disease

WANG Liangping

2012, 27(11):  944-947. 

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Objective　To investigate the roles of serum oxidized low density lipoprotein（ox-LDL）/beta2-glycoprotein Ⅰ（β2-GPI） complex in type 2 diabetes mellitus（T2DM）patients complicated with coronary heart disease（CHD）and its clinical significance. Methods　Serum samples were collected from 68 T2DM patients complicated with CHD，69 patients with T2DM，65 patients with CHD and 60 healthy controls.Serum ox-LDL/β2-GPI complex were measured by enzyme-linked immunosorbent assay（ELISA），and the fasting blood glucose（FBG），total cholesterol（TC），triglyceride（TG），high-density lipoprotein holesterol（HDL-C），low-density lipoprotein cholesterol（LDL-C），glycosylated hemoglobin（HbA1C），fasting serum insulin（FINS），body mass index（BMI）and waist to hip ratio（WHR）were determined and analyzed. Results　The T2DM patients complicated with CHD had significantly higher levels of ox-LDL/β2-GPI complex than T2DM group，CHD group and control group（P<0.05）.The ox-LDL/β2-GPI complex level was positively correlated with TG and homeostasis model assessment insulin resistance（HOMA-IR） index in the T2DM patients complicated with CHD（r=0.219 and 0.228，P<0.01）.TG and ox-LDL/β2-GPI complex were the risk factors of T2DM.The ox-LDL/β2-GPI complex and HbA1Cwere the risk factors of T2DM complicated with CHD. Conclusions　Serum ox-LDL/β2-GPI complex may play an important role in the development of T2DM complicated with CHD.

The clinical significance of glycated albumin for patients with liver cirrhosis

JIANG Xiaojiong，SUN Xiansheng，HUANG Yiqing，XU Dajun

2012, 27(11):  948-950. 

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Objective　To investigate the clinical significance and change of glycated albumin（GA） for patients with liver cirrhosis.   Methods　The percentage of GA（GA%） and the level of glucose（Glu） in 105 patients with liver cirrhosis and 80 healthy controls were detected by enzyme method and hexokinase（HK） method. A total of 105 patients with liver cirrhosis were classified into 2 groups according to the Child-Pugh criterion，86 patients in Child-Pugh A group and 19 patients in Child-Pugh B+C group. The differences of GA% and Glu between the 2 groups were analyzed. Results　GA% increased significantly in liver cirrhosis group，compared with that in control group（P<0.01）. There was no statistical significance of the level and abnormal rate of Glu between the 2 groups（P>0.05）. Furthermore，along with the progression of liver disease，the abnormal rate of GA% increased obviously，and that of Glu had no significant difference. Conclusions　GA% in liver cirrhosis patients with normal Glu increases abnormally and is closely related to the disease progression.