Abstract:  Objective　To increase the detection rate of Salmonella in intestinal tract by the improved isolation and identification method，and to fundamentally understand the major Salmonella and its molecular typing in Shanghai，establish the database of pulsed-field gel electrophoresis（PFGE）typing for rapid source-tracing and control dissemination of the disease during Salmonella outbreaks. Methods　The improved isolation and identification method was used to detect suspected Salmonella in fecal specimens. PFGE was used to perform molecular typing of Salmonella typhimurium. Results　Salmonella enteritidis was main among the isolates of Salmonella by the improved isolation and identification method，accounting for 42.1%.The second was Salmonella typhimurium，accounting for 13.2%.Two distinct DNA fragment profiles，PFGE type A and B，were observed，including type A: 6 isolates and type B: 4 isolates respectively.Type A could be subclassified further into 4 subtypes. Conclusions　The improved isolation and identification method could effectively increase the detection rate of Salmonella in fecal specimens.PFGE is an effective method to study the molecular typing.The research shows relatively close kinship in molecular typing of Salmonella typhimurium.

Key words: Salmonella, Improved isolation and identification method, Pulsed-field gel electrophoresis, Molecular typing