Identification and antimicrobial susceptibility of Salmonella typhimurium monophasic variant in Shanghai

doi:10.3969/j.issn.1673-8640.2018.09.006

Abstract

Abstract:

Objective To investigate the genotypes and antimicrobial susceptibility of monophasic variant of Salmonella typhimurium （monophasic Salmonella typhimurium） in Shanghai from 2013 to 2015. Methods A total of 1 179 isolates of Salmonella isolated from diarrheal patients from 2013 to 2015 and stored by bacter testing laboratory in Shanghai Municipal Center for Disease Control and Prevention were collected. Serotyping and polymerase chain reaction （PCR） were used to screen and identify monophasic Salmonella typhimurium. The genotype was analyzed by PCR. Antimicrobial susceptibility was evaluated by Kirby-Bauer disk diffusion method with 14 antimicrobial agents for monophasic and diphasic Salmonella typhimurium,in accordance with the Clinical and Laboratory Standards Institute （CLSI） guidelines. Results A total of 52 isolates were identified as monophasic Salmonella typhimurium from 1 179 isolates of Salmonella. The percentages of monophasic Salmonella typhimurium in all isolates of Salmonella and Salmonella typhimurium were 4.4% and 23.3%,respectively. The determination rate of monophasic Salmonella typhimurium was high in young- and middle-aged groups. Most of them were from the suburbs of Shanghai. In the 52 isolates of monophasic Salmonella typhimurium,44 isolates （84.6%） were confirmed as fljB gene positive,and the remaining 8 isolates were fljB⁺ fljA^- hin^-（2 isolates,3.8%） and fljB⁺ fljA⁺hin⁺ （6 isolates,11.6%）. There were 40 isolates （76.9%） of monophasic Salmonella typhimurium resistant to 3 or more antimicrobials. There were 36 isolates （69.2%） resistant to more than 4 antimicrobials. The drug resistance rates to ampicillin-streptomycin-sulfonamide-tetracycline（ASSuT） and ampicillin-chloramphenicol-streptomycin-sulfonamide-tetracycline（ACSSuT） were 61.54% and 19.23%,respectively,which were higher than those of diphasic Salmonella typhimurium （P<0.05）. Conclusions The determination rate of monophasic Salmonella typhimurium is high in Shanghai with multi-drug resistance and uneven population distribution. It should be paid attention to the reasonable distribution of resources and the rational use of antimicrobials.

Key words: Salmonella typhimurium, Drug resistance, Genotype, Monophasic variant

CLC Number:

R446.5

WU Wen, TU Lihong, ZHANG Wenxia, ZHANG Xi, CHEN Min. Identification and antimicrobial susceptibility of Salmonella typhimurium monophasic variant in Shanghai[J]. Laboratory Medicine, 2018, 33(9): 798-802.

Figures/Tables 3

References 14

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基因名称	引物序列（5'~3'）	退火温度（°C）	目的片段大小（bp）
fliB-fliA	CTGGCGACGATCTGTCGATG	58	964
	GCGGTATACAGTGAATTCAC
fljA	TTCATTAGGTCCCCTCCGG	55	642
	ATTCAGCCCCGTGAATTCGGG
fljB	TTTACCGTCTACGCCACCC	52	561
	GGTACTACACTGGATGTATCGGG
hin	TGGCTACTATTGGGTATATTCGGG	52	570
	AATTCATTCGTTTTTTTATGCGGC

基因名称	引物序列（5'~3'）	退火温度（°C）	目的片段大小（bp）
fliB-fliA	CTGGCGACGATCTGTCGATG	58	964
	GCGGTATACAGTGAATTCAC
fljA	TTCATTAGGTCCCCTCCGG	55	642
	ATTCAGCCCCGTGAATTCGGG
fljB	TTTACCGTCTACGCCACCC	52	561
	GGTACTACACTGGATGTATCGGG
hin	TGGCTACTATTGGGTATATTCGGG	52	570
	AATTCATTCGTTTTTTTATGCGGC

耐药谱	单相株耐药率	双相株耐药率
ASSuT±其他	61.54	19.7^*
ACSSuT±其他	19.23	10.6^*
3类以上耐药	76.92	48.48
4类以上耐药	69.23	34.8

耐药谱	单相株耐药率	双相株耐药率
ASSuT±其他	61.54	19.7^*
ACSSuT±其他	19.23	10.6^*
3类以上耐药	76.92	48.48
4类以上耐药	69.23	34.8

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