Laboratory Medicine ›› 2018, Vol. 33 ›› Issue (12): 1098-1102.DOI: 10.3969/j.issn.1673-8640.2018.12.009
• Orginal Article • Previous Articles Next Articles
ZHANG Jinghao1, YANG Feng1, FANG Yi1, YANG Lihua2, LIU Wenjian1, ZHAO Hu1, ZHANG Yanmei1
Received:
2018-03-26
Online:
2018-12-26
Published:
2018-12-27
CLC Number:
ZHANG Jinghao, YANG Feng, FANG Yi, YANG Lihua, LIU Wenjian, ZHAO Hu, ZHANG Yanmei. Drug resistance profile and related genes of Salmonella isolated from patients with acute infectious diarrhea[J]. Laboratory Medicine, 2018, 33(12): 1098-1102.
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URL: https://www.shjyyx.com/EN/10.3969/j.issn.1673-8640.2018.12.009
目的基因 | 序列((5'~3') | 片段长度(bp) |
---|---|---|
blaTEM-1 | F:GTGCTGCCATAACCATGAGT | 282 |
R:CTGCAACTTTATCCGCCTCC | ||
blaCMY-2 | F:TTACTGCGTTATTTATTCCCAC | 339 |
R:CGCCCAAAATGACTTTAGCAA | ||
blaPSE-1 | F:TGCTTCGCAACTATGACTAC | 438 |
R:AGCCTGTGTTTGAGCTAGAT | ||
blaCTX-M1 | F:CGTCACGCTGTTGTTAGGAA | 780 |
R:ACGGCTTTCTGCCTTAGGTT | ||
blaCTX-M2 | F:ACGCTACCCCTGCTATT | 830 |
R:CAGAAACCGTGGGTTACGA | ||
blaCTX-M8 | F:AGTGCAACGGATGATGT | 792 |
R:GGCTGGGTAAAATAGGTC | ||
blaCTX-M9 | F:TATTGGGAGTTTGAGATGGT | 932 |
R:TCCTTCAACTCAGCAAAAGT | ||
blaSHV | F:AGCCGCTTGAGCAAATTAAAC | 713 |
R:ATCCCGCAGATAAATCACCAC | ||
DHA | F:AACTTTCACAGGTGTGCTGGGT | 405 |
R:CCGTACGCATACTGGCTTTGC | ||
sul I | F:CACAGCCAAACTATCAGGTCAA | 353 |
R:TACCTACAAAGCCCCACGCATC | ||
sulⅡ | F:GGCTTATTTTCCTGCTAATTGG | 710 |
R:GCAATGTGATCCATGATGTCGC | ||
sulⅢ | F:GGGAGCCGCTTCCAGTAAT | 500 |
R:TCCGTGACACTGCAATCATTA | ||
aph(3')-Ⅱ a | F:CACATCCGAACATAAACAACC | 244 |
R:CCGTCAGCCAGTTTAGTCT | ||
aadA1 | F:CATCTCGAACCGACGTTGCT | 492 |
R:TACATTTCGCTCATCGCCAG | ||
aadB | F:CATGGACACAACGCAGGTCACA | 508 |
R:GGCACGCAAGACCTCAACCTTT | ||
strB | F:GCGGACACCTTTTCCAGCCT | 608 |
R:TCCGCCATCTGTGCAATGGCG | ||
qyrA | F:CGTTGGTGACGTAATCGGTA | 190 |
R:CCGTACCGTCATAGTTATCC | ||
qnrB | F:GATCGTGAAAGCCAGAAAGG | 469 |
R:ATGAGCAACGATGCCTGGTA | ||
qnrC | F:GGGTTGTACATTTATTGAATC | 447 |
R:TCCACTTTACGAGGTTCT | ||
qnrD | F:GGGGATATCTTAAGGTTGTTCAAATTAATGTAC | 645 |
R:CCCGTCGACTTTGATTAGTACCA CATTGG | ||
qnrS | F:GCAAGTTCATTGAACAGGGT | 428 |
R:TCTAAACCGTCGAGTTCGGCG | ||
parC | F:CTATGCGATGTCAGAGCTGG | 270 |
R:TAACAGCAGCTCGGCGTATT |
目的基因 | 序列((5'~3') | 片段长度(bp) |
---|---|---|
blaTEM-1 | F:GTGCTGCCATAACCATGAGT | 282 |
R:CTGCAACTTTATCCGCCTCC | ||
blaCMY-2 | F:TTACTGCGTTATTTATTCCCAC | 339 |
R:CGCCCAAAATGACTTTAGCAA | ||
blaPSE-1 | F:TGCTTCGCAACTATGACTAC | 438 |
R:AGCCTGTGTTTGAGCTAGAT | ||
blaCTX-M1 | F:CGTCACGCTGTTGTTAGGAA | 780 |
R:ACGGCTTTCTGCCTTAGGTT | ||
blaCTX-M2 | F:ACGCTACCCCTGCTATT | 830 |
R:CAGAAACCGTGGGTTACGA | ||
blaCTX-M8 | F:AGTGCAACGGATGATGT | 792 |
R:GGCTGGGTAAAATAGGTC | ||
blaCTX-M9 | F:TATTGGGAGTTTGAGATGGT | 932 |
R:TCCTTCAACTCAGCAAAAGT | ||
blaSHV | F:AGCCGCTTGAGCAAATTAAAC | 713 |
R:ATCCCGCAGATAAATCACCAC | ||
DHA | F:AACTTTCACAGGTGTGCTGGGT | 405 |
R:CCGTACGCATACTGGCTTTGC | ||
sul I | F:CACAGCCAAACTATCAGGTCAA | 353 |
R:TACCTACAAAGCCCCACGCATC | ||
sulⅡ | F:GGCTTATTTTCCTGCTAATTGG | 710 |
R:GCAATGTGATCCATGATGTCGC | ||
sulⅢ | F:GGGAGCCGCTTCCAGTAAT | 500 |
R:TCCGTGACACTGCAATCATTA | ||
aph(3')-Ⅱ a | F:CACATCCGAACATAAACAACC | 244 |
R:CCGTCAGCCAGTTTAGTCT | ||
aadA1 | F:CATCTCGAACCGACGTTGCT | 492 |
R:TACATTTCGCTCATCGCCAG | ||
aadB | F:CATGGACACAACGCAGGTCACA | 508 |
R:GGCACGCAAGACCTCAACCTTT | ||
strB | F:GCGGACACCTTTTCCAGCCT | 608 |
R:TCCGCCATCTGTGCAATGGCG | ||
qyrA | F:CGTTGGTGACGTAATCGGTA | 190 |
R:CCGTACCGTCATAGTTATCC | ||
qnrB | F:GATCGTGAAAGCCAGAAAGG | 469 |
R:ATGAGCAACGATGCCTGGTA | ||
qnrC | F:GGGTTGTACATTTATTGAATC | 447 |
R:TCCACTTTACGAGGTTCT | ||
qnrD | F:GGGGATATCTTAAGGTTGTTCAAATTAATGTAC | 645 |
R:CCCGTCGACTTTGATTAGTACCA CATTGG | ||
qnrS | F:GCAAGTTCATTGAACAGGGT | 428 |
R:TCTAAACCGTCGAGTTCGGCG | ||
parC | F:CTATGCGATGTCAGAGCTGG | 270 |
R:TAACAGCAGCTCGGCGTATT |
抗菌药物 | 敏感 | 中介 | 耐药 |
---|---|---|---|
氨苄西林 | 4(18.2) | 0(0.0) | 18(81.8) |
氨苄西林-舒 巴坦 | 1(4.5) | 0(0.0) | 21(95.5) |
哌拉西林-他 唑巴坦 | 22(100.0) | 0(0.0) | 0(0.0) |
头孢唑林 | 19(86.4) | 0(0.0) | 3(13.6) |
头孢呋辛 | 19(86.4) | 0(0.0) | 3(13.6) |
头孢噻肟 | 16(72.7) | 2(9.1) | 4(18.2) |
头孢他啶 | 19(86.4) | 0(0.0) | 3(13.6) |
头孢吡肟 | 21(95.5) | 1(4.5) | 0(0.0) |
头孢西丁 | 21(95.5) | 1(4.5) | 0(0.0) |
亚胺培南 | 22(100.0) | 0(0.0) | 0(0.0) |
阿米卡星 | 21(95.5) | 0(0.0) | 1(4.5) |
庆大霉素 | 18(81.8) | 0(0.0) | 4(18.2) |
环丙沙星 | 4(18.2) | 14(63.6) | 4(18.2) |
左氧氟沙星 | 5(22.7) | 0(0.0) | 17(77.3) |
复方磺胺 甲噁唑 | 16(72.7) | 0(0.0) | 6(27.3) |
抗菌药物 | 敏感 | 中介 | 耐药 |
---|---|---|---|
氨苄西林 | 4(18.2) | 0(0.0) | 18(81.8) |
氨苄西林-舒 巴坦 | 1(4.5) | 0(0.0) | 21(95.5) |
哌拉西林-他 唑巴坦 | 22(100.0) | 0(0.0) | 0(0.0) |
头孢唑林 | 19(86.4) | 0(0.0) | 3(13.6) |
头孢呋辛 | 19(86.4) | 0(0.0) | 3(13.6) |
头孢噻肟 | 16(72.7) | 2(9.1) | 4(18.2) |
头孢他啶 | 19(86.4) | 0(0.0) | 3(13.6) |
头孢吡肟 | 21(95.5) | 1(4.5) | 0(0.0) |
头孢西丁 | 21(95.5) | 1(4.5) | 0(0.0) |
亚胺培南 | 22(100.0) | 0(0.0) | 0(0.0) |
阿米卡星 | 21(95.5) | 0(0.0) | 1(4.5) |
庆大霉素 | 18(81.8) | 0(0.0) | 4(18.2) |
环丙沙星 | 4(18.2) | 14(63.6) | 4(18.2) |
左氧氟沙星 | 5(22.7) | 0(0.0) | 17(77.3) |
复方磺胺 甲噁唑 | 16(72.7) | 0(0.0) | 6(27.3) |
抗菌药物 种类 | 目的基因 | 检出率(%) | |
---|---|---|---|
耐药 | 敏感 | ||
β-内酰胺类 | blaTEM-1 | 23.80 | 0.00 |
blaCMY-2 | 0.00 | 0.00 | |
blaPSE-1 | 0.00 | 0.00 | |
blaCTX-M1 | 0.00 | 0.00 | |
blaCTX-M2 | 0.00 | 0.00 | |
blaCTX-M8 | 0.00 | 0.00 | |
blaCTX-M9 | 0.00 | 0.00 | |
blaSHV | 0.00 | 0.00 | |
DHA | 23.80 | 0.00 | |
磺胺类 | sul I | 66.70 | 0.00 |
sulⅡ | 0.00 | 6.25 | |
sulⅢ | 16.70 | 0.00 | |
氨基糖苷类 | aph(3')-Ⅱ a | 0.00 | 5.60 |
aadA1 | 25.00 | 22.20 | |
aadB | 0.00 | 5.60 | |
strB | 0.00 | 50.00 | |
喹诺酮类 | qyrA | 47.10 | 0.00 |
parC | 58.80 | 0.00 | |
qnrA | 5.90 | 20.00 | |
qnrB | 11.70 | 0.00 | |
qnrC | 0.00 | 0.00 | |
qnrS | 17.60 | 0.00 |
抗菌药物 种类 | 目的基因 | 检出率(%) | |
---|---|---|---|
耐药 | 敏感 | ||
β-内酰胺类 | blaTEM-1 | 23.80 | 0.00 |
blaCMY-2 | 0.00 | 0.00 | |
blaPSE-1 | 0.00 | 0.00 | |
blaCTX-M1 | 0.00 | 0.00 | |
blaCTX-M2 | 0.00 | 0.00 | |
blaCTX-M8 | 0.00 | 0.00 | |
blaCTX-M9 | 0.00 | 0.00 | |
blaSHV | 0.00 | 0.00 | |
DHA | 23.80 | 0.00 | |
磺胺类 | sul I | 66.70 | 0.00 |
sulⅡ | 0.00 | 6.25 | |
sulⅢ | 16.70 | 0.00 | |
氨基糖苷类 | aph(3')-Ⅱ a | 0.00 | 5.60 |
aadA1 | 25.00 | 22.20 | |
aadB | 0.00 | 5.60 | |
strB | 0.00 | 50.00 | |
喹诺酮类 | qyrA | 47.10 | 0.00 |
parC | 58.80 | 0.00 | |
qnrA | 5.90 | 20.00 | |
qnrB | 11.70 | 0.00 | |
qnrC | 0.00 | 0.00 | |
qnrS | 17.60 | 0.00 |
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