Abstract: Objective　To compare the results of anti-herpes simplex virus type 2（HSV-2）IgG antibody detected by electro-chemiluminescence assay（ECLIA）and enzyme-linked immunosorbent assay（ELISA），and evaluate the performance and application significance of ECLIA. Methods　Anti-HSV-2 specific IgG antibody of 400 serum samples were detected by ECLIA（Roche）and ELISA（Trinity，Ireland）. The samples with inconsistent results were detected by anti-HSV-2 IgG antibody detection kit of Euroimmun Biotechnology Co.Ltd.. Results　The positive rates were 39.0% by ELISA and 37.8% by ECLIA respectively. Compared with ELISA，the false negative rate of ECLIA was 1.3%，and the false positive rate was 0.8%. The relative sensitivity and relative specificity were 96.8% and 98.8%. The coincidence rate of the 2 methods was 98.0%. The 13 samples with inconsistent results（including 2 uncertain results）were detected by anti-HSV-2 IgG antibody detection kit of Euroimmun Biotechnology Co.，Ltd.. The results indicated that 6 samples were consistent with ECLIA，and 5 samples were consistent with ELISA. Conclusions　ECLIA and ELISA results had good coincidence，and Roche ECLIA can be used as a fast，accurate，sensitive，high-specific reagent for clinical application in the determination of anti-HSV-2 IgG antibody.

Key words: Herpes simplex virus type 2, IgG antibody, Electro-chemiluminescence assay, Enzyme-linked immunosorbent assay