False negative interference of RF on serum HBeAg by ELISA

doi:10.3969/j.issn.1673-8640.2017.09.005

Abstract

Abstract:

Objective To investigate whether rheumatoid factor（RF） can cause false negative interference on serum hepatitis B e antigen（HBeAg）by enzyme-linked immunosorbent assay（ELISA）.Methods Serum samples with negative hepatitis B surface antigen（HBsAg）,negative HBeAg and positive RF were collected and comprised by blending HBeAg-positive and RF-positive control substance at a ratio of 9∶1. HBeAg level was determined by different ELISA kits. RF adsorption latex was used to remove RF from samples,and the change of HBeAg level was compared before and after pre-treatment. Results The 3 kinds of HBeAg ELISA kits had false negative interference. There were 7 of 9 false negative samples transforming to positive after pre-treatment. Conclusions The S/CO of HBeAg determination by ELISA is susceptible to false negative interference from RF. The pre-treatment of samples is effective prior to the interpretation of determination results.

Key words: Rheumatoid factor, Interference, Enzyme-linked immunosorbent assay, Hepatitis B e antigen

CLC Number:

R446.62

XIE Liangcai, FAN Wen, XU Lei, XU Rong, LIU Tianchun. False negative interference of RF on serum HBeAg by ELISA[J]. Laboratory Medicine, 2017, 32(9): 765-768.

Figures/Tables 6

References 15

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组别	假阴性（S/CO值<1.00）			合计
组别	试剂Ⅰ	试剂Ⅱ	试剂Ⅲ	合计
弱阳性组	18/53	21/53	26/53	65/159
阳性组	4/49	6/49	7/49	17/147
强阳性组	0/55	0/55	0/55	0/165
合计	26/157	30/157	37/157

组别	假阴性（S/CO值<1.00）			合计
组别	试剂Ⅰ	试剂Ⅱ	试剂Ⅲ	合计
弱阳性组	18/53	21/53	26/53	65/159
阳性组	4/49	6/49	7/49	17/147
强阳性组	0/55	0/55	0/55	0/165
合计	26/157	30/157	37/157

组别	不同HBeAg水平的S/CO值
组别	水平1（43例）	水平2（24例）	水平3（25例）	水平4（41例）	水平5（24例）
试验组	1.17±0.80	1.49±0.91	8.09±3.48	13.47±6.57	22.43±9.40
对照组	1.63±0.15	2.40±0.17	8.34±0.56	19.60±1.03	29.80±2.37
P值	0.001	0.000	0.731	0.000	0.001

组别	不同HBeAg水平的S/CO值
组别	水平1（43例）	水平2（24例）	水平3（25例）	水平4（41例）	水平5（24例）
试验组	1.17±0.80	1.49±0.91	8.09±3.48	13.47±6.57	22.43±9.40
对照组	1.63±0.15	2.40±0.17	8.34±0.56	19.60±1.03	29.80±2.37
P值	0.001	0.000	0.731	0.000	0.001

样本	各稀释比例样本的HBeAg S/CO值
样本	原倍	1∶2	1∶4	1∶8	1∶16	1∶32
试验组
样本1	0.82	0.83	1.14	1.33	0.90	0.71
样本2	2.31	1.84	1.77	1.66	1.79	0.86
样本3	3.63	3.26	2.88	2.04	1.52	0.84
样本4	1.23	1.14	1.07	1.05	0.91	0.91
样本5	2.21	2.31	2.01	1.85	1.40	0.93
对照组
对照1	15.82	9.29	5.38	3.08	1.59	0.84
对照2	16.23	10.03	6.02	3.11	1.68	0.86