Abstract:

Objective To investigate the interference effect of hemolysis,icterus and lipemia on the determination of prealbumin（PA） by immunoscattering turbidimetry. Methods A fresh serum sample was used as a basic sample,interfering substances [hemoglobin（Hb）,bilirubin and lipid] were added,and the PA level was determined by immunoscattering turbidimetry. According to the EP07-A3 document of the Clinical and Laboratory Standards Institute（CLSI）,the paired difference experiment and dose-effect experiment were performed. Results PA had no interference with 10 g/L Hb（hemolysis index of 1 000）and 200 mg/L（340 μmol/L） bilirubin（icterus index of 20）. The lipid turbidity interfering substance with a lipid turbidity index of 987 had a negative interference on the determination of PA. The degree of interference had a linear relationship with the lipid turbidity index. For samples with low values of PA,when the lipid turbidity index≤120,there was no interference to PA determination. The linear equation between lipid turbidity index and interference rate was Y=-0.077X+0.979（r ²=0.999）. For samples with high values of PA,when the lipid turbidity index≤203,there was no interference to PA determination. The linear equation between lipid turbidity index and interference rate was Y=-0.040X-0.168（r²=0.992）. Conclusions Generally clinical hemolytic and icteric samples will not interfere with the PA determination results by immunoscattering turbidimetry. The samples with lipid turbidity index>120 will cause negative interference to PA determination results.

Key words: Prealbumin, Immunoscattering turbidimetry, Serum index, Interference