Comparative study on genotyping of Candida tropicalis by repetitive sequence-based PCR and multilocus sequence typing

Abstract

Abstract: Objective　To compare repetitive sequence-based polymerase chain reaction（REP-PCR）and multilocus sequence typing（MLST）in genotyping of Candida tropicalis. Methods　REP-PCR was performed on 147 clinical isolates of Candida tropicalis collected from 6 hospitals of 5 provinces.Primer Ca-21，Ca-22 and Com-21 were used pairly to find the most suitable pair.Three isolates of Candida tropicalis from different REP-PCR types were tested by MLST.Six loci in housekeeping genes were sequenced after amplification，which were compared with the MLST database to obtain sequence type（ST）. Results　Eight REP-PCR types were found in 147 isolates of Candida tropicalis with primer Com21-Com21，which had the best genotyping effect.Type A-H were corresponding with ST146，NEW1，ST136，ST127，ST177，ST169，NEW2 and ST117 by MLST respectively. Conclusions　REP-PCR offers a simple and rapid method for molecular typing，which has a similar discriminatory power with MLST.Therefore，REP-PCR can be the first choice in laboratory，especially for a large number of isolates.

Key words: Candida tropicalis, Repetitive sequence-based polymerase chain reaction, Multilocus sequence typing, Genotyping

JIANG Cen，DONG Danfeng，YU Beiqin，PENG Yibing. Comparative study on genotyping of Candida tropicalis by repetitive sequence-based PCR and multilocus sequence typing[J]. , 2012, 27(11): 917-920.

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