Abstract: Objective　To evaluate A and B ELISA kits for detecting qualitatively anti-hepatitis B e antigen antibody（HBeAb），and to provide useful reference for choosing suitable HBeAb kits. Methods　According to Clinical and Laboratory Standards Institute（CLSI） EP12-A2 document，the bias and imprecision of A and B HBeAb kits were analyzed，and their C50，C5-C95 intervals and imprecision curves were compared.The HBeAb of 910 specimens were simultaneously determined by both A and B kits.The 95% confidence interval（CI）for concordance was calculated.The Kappa value was calculated to evaluate their concordance. Results　C50 for A kit was less than that for B kit.C5-C95 interval of A kit was narrower than that of B kit.The imprecision curve of A kit was steeper than that of B kit.The 95%CI for concordance was calculated as 93.2%-96.1%，and the Kappa value was 0.87. Conclusions　EP12-A2 is a useful protocol for the evaluation of qualitative test performance.

Key words: Performance evaluation, Qualitative test, EP12-A2, Anti-hepatitis B e antigen antibody