关键词: 中枢神经特异蛋白, 老年性痴呆, 酶联免疫吸附试验

Abstract:

Objective To evaluate the role of central nervous system specific protein（s100β protein） determination in senile dementia. Methods A total of 300 patients with Alzheimer's disease were enrolled,and 200 healthy subjects with same age were enrolled as healthy control group. Their blood samples were collected,and serum s100β protein levels were determined by enzyme-linked immunosorbent assay（ELISA）. The cognitive function was evaluated by mini-mental state examination（MMSE）,and the relationship between s100β protein and MMSE score was evaluated. Results The level of serum s100β protein in observation group was（82.07±11.58）pg/mL,and it was higher than that in healthy control group [（49.16±2.23）pg/mL,P<0.05]. The sensitivity of s100β protein determination was 71.35%,and the specificity was 84.67%. The MMSE score in observation group was 15.93±4.68,and it was lower than that in healthy control group（26.17±3.02,P<0.05）. There was a negative correlation between s100β protein and MMSE score [correlation coefficient（r）=-0.276,P<0.05]. Conclusions The determination of s100β protein for senile dementia has high sensitivity and specificity. The higher serum s100β protein level is,more serious the degree of senile dementia is. It can be used for the auxiliary diagnosis of senile dementia.

Key words: Central nervous system specific protein, Senile dementia, Enzyme-linked immunosorbent assay