关键词: 抗M2型线粒体抗体, 原发性胆汁性肝硬化, 酶联免疫吸附试验

Abstract:

Objective To screen specific anti-mitochondrial antibody （AMA）-M2 for primary biliary cirrhosis （PBC） in healthy subjects,to analyze the results of positive cases,and to evaluate the role of AMA-M2 for the early screening of PBC. Methods AMA-M2 in 36 459 healthy subjects were determined by enzyme-linked immunosorbent assay （ELISA）. The results of AMA-M2 positive cases were analyzed further. Imaging examination was used to exclude non-PBC abnormality and make diagnosis. Results A total of 438 AMA-M2 positive cases were determined. The positive rate was 1.20%. There were 0.72% males （135/18 687） and 1.70% females （303/17 772）. In the 438 AMA-M2 positive cases,39 cases had alkaline phosphatase （ALP） increasing,3 cases with gamma-glutamyltransferase （GGT） increasing,15 cases with alanine aminotransferase （ALT）increasing,7 cases with total bilirubin （TB） increasing,7 cases with direct bilirubin （DBil） increasing and 4 cases with indirect bilirubin （IBil）increasing. Combined with the results of biochemical determinations,there were 39 cases meeting the diagnostic criteria of PBC. The prevalence rate was 0.11%,in which 10 males were （59.40±13.93）years old and 29 females were （54.31±14.80） years old. Conclusions Asymptomatic patients with PBC are common in Guiyang. Carrying out AMA-M2 screening in healthy population is an effective approach for the early determination of PBC.

Key words: Anti-mitochondrial antibody-M2, Primary biliary cirrhosis, Enzyme-linked immunosorbent assay