检验医学 ›› 2019, Vol. 34 ›› Issue (7): 610-612.DOI: 10.3969/j.issn.1673-8640.2019.07.008

• 临床应用研究·论著 • 上一篇    下一篇

PLT-F通道在异常血小板计数检测中的价值

袁莉, 邓凯, 李娜, 仝晓宁   

  1. 西安交通大学第一附属医院检验科,陕西 西安 710061
  • 收稿日期:2018-08-25 出版日期:2019-07-30 发布日期:2019-07-25
  • 作者简介:null

    作者简介:袁莉,女,1982年生,硕士,主管技师,主要从事临床血液学检验工作。

Role of PLT-F channel in abnormal platelet count determination

YUAN Li, DENG Kai, LI Na, TONG Xiaoning   

  1. Department of Clinical Laboratory,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,Shaanxi,China
  • Received:2018-08-25 Online:2019-07-30 Published:2019-07-25

摘要:

目的 通过荧光血小板(PLT-F)通道对异常血小板(PLT)计数样本进行检测,分析PLT-F通道在异常PLT计数检测中的价值。方法 采集空腹静脉血样本158例,将样本分为低值PLT组、小红细胞干扰组、大PLT组和PLT聚集组。采用电阻抗法(PLT-I)、荧光法(PLT-F)检测PLT计数,并将其检测结果与人工镜检法(PLT-M)结果进行比较。结果 低值PLT组、大PLT组及小红细胞干扰组PLT-F通道检测PLT计数及幼稚血小板分数(IPF)的批内重复性[变异系数(CV)]分别为4.94%、1.70%、0.80%与4.81%、1.30%、2.74%。低值PLT组、小红细胞干扰组及大PLT组PLT-F与PLT-M的决定系数(r2)值分别为0.856 8、0.822 3与0.771 0;PLT-I与PLT-M的r2值分别为0.679 1、0.775 4与0.592 6。结论 PLT-F通道批内重复性好,可通过PLT-F通道对异常PLT计数样本进行快速复检。

关键词: 荧光血小板通道, 血液分析仪, 异常血小板计数, 人工镜检法

Abstract:

Objective To evaluate the role of fluorescent platelet(PLT-F) channel in the determination of abnormal platelet(PLT) count. Methods A total of 158 fasting peripheral blood specimens were collected,which were classified into low PLT group,small red blood cell interference group,large PLT group and PLT aggregation group. Using impedance(PLT-I),fluorescence(PLT-F) and manual microscopy(PLT-M) methods,PLT counts were determined. The PLT-I and PLT-F results were compared with those of PLT-M. Results The within-run repeatabilities [coefficients of variation(CV)] of PLT count and immature platelet fraction(IPF) in low PLT,large PLT and small red blood cell interference groups were 4.94%,1.70%,0.80% and 4.81%,1.30%,2.74%,respectively. The determination coefficients(r2) of PLT-F with PLT-M in low PLT group,small red blood cell interference group and large PLT group were 0.856 8,0.822 3 and 0.771 0,respectively,and those of PLT-I with PLT-M were 0.679 1,0.775 4 and 0.592 6,respectively. Conclusions PLT-F channel has good within-run repeatability. PLT-F channel is a rapid and accurate retest way on determining abnormal PLT count.

Key words: Fluorescent platelet channel, Hematology analyzer, Abnormal platelet count, Manual microscopy

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