Table of Content

30 August 2020, Volume 35 Issue 8

Previous Issue    Next Issue

Study on the judgment model of preoperative microvascular invasion in HCC based on common clinical determination items

TONG Lin, HUANG Chenjun, GAO Zhiyuan, ZHOU Jun, FANG Meng, XIAO Xiao, HE Yutong, HONG Song, XU Minfan, ZHU Feifei, GAO Chunfang

2020, 35(8):  741-748.  DOI: 10.3969/j.issn.1673-8640.2020.08.001

Asbtract ( 126 )   HTML ( 5)   PDF (1074KB) ( 134 )  

Figures and Tables | References | Related Articles | Metrics

Objective To establish a judgment model of microvascular invasion（MVI） in hepatocellular carcinoma（HCC） based on common clinical determination items. Methods A total of 5 602 patients with HCC were enrolled as training group,and 1 905 patients with HCC were enrolled as validation group. The clinical data,including sex,age,tumor number,tumor size,Edmondson grade,MVI occurrence and preoperative 48 indexes,were collected. In training group, Logistic regression analysis was used to evaluate the risk factors of MVI in patients with HCC before operation,a judgment model was established,and independent validation was performed in validation group. Receiver operating characteristic（ROC） curve was used to evaluate the performance of the judgment model,PA-SALAD,for the diagnosis of MVI occurrence in preoperative HCC patients. Results There was statistical significance in age,albumin（Alb）,alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,gamma-glutamyltransferase（GGT）,alkaline phosphatase（ALP）,alpha-L-fucosidase（AFU）,sialic acid（SA）,prothrombin time（PT）,tumor number,tumor size and MVI ratio between training and validation groups（P<0.05）. There was no statistical significance for the other indexes between the 2 groups（P>0.05）. Logistic regression analysis showed that age,platelet（PLT）,Alb,SA,alpha-fetoprotein（AFP）,des-gamma-carboxy prothrombin（DCP） and alpha-fetoprotein heterogeneity（AFP-L3） were risk factors for MVI occurrence [odds ratios（OR） were 0.981,0.998,0.971,1.016,1.226,1.361 and 1.012]. Based on the results of Logistic regression analysis,the judgment model,PA-SALAD=0.220-0.002×PLT-0.019×age+0.016×SA-0.029×Alb+0.012×AFP-L3+0.204×lgAFP+0.308×lgDCP,was established. ROC curve analysis showed that the area under curve（AUC）,sensitivity and specificity of PA-SALAD for discriminating MVI in training group were 0.695,69.3% and 60.4%,and the optimal cut-off value was -0.235 3. In validation group,the AUC,sensitivity and specificity were 0.704,66.5% and 62.0%,and the optimal cut-off value was -0.235 3. Conclusions The judgment model,PA-SALAD,based on common clinical determination items is of certain value to determine whether MVI occurs in HCC patients,especially for patients at high risk of MVI.

Establishment of reference intervals of total bilirubin and direct bilirubin by indirect method

WU Qun, SHEN Junfei, WU Wenhao, WANG Beili, PAN Baishen, GUO Wei

2020, 35(8):  749-752.  DOI: 10.3969/j.issn.1673-8640.2020.08.002

Asbtract ( 206 )   HTML ( 12)   PDF (807KB) ( 225 )  

Figures and Tables | References | Related Articles | Metrics

Objective Using indirect method,to establish the reference intervals of total bilirubin（TB） and direct bilirubin（DBil）,to compare with the reference interval of industry standard（direct method）,and to verify its reliability. Methods The data of healthy subjects were collected from January 2017 to December 2017. There were 52 589 males and 36 819 females for TB data,and there were 51 555 males and 36 228 females for DBil data. Using skewness-kurtosis test,the normality of data was analyzed. If not the original data were transformed through BOX-COX transformation to obtain an approximate normal distribution,outliers were identified and omitted by turkey method. The indirect reference intervals were taken by Hoffmann method and compared with the reference interval of direct method. Results The results of skewness-kurtosis test showed that TB and DBil data were non-normal distribution. Using BOX-COX transformation to transform data into normality,784 males and 578 females of TB data and 925 males and 675 females of DBil data were eliminated by turkey method after transformation. There was statistical significance for TB and DBil between males and females（P=0.000）. Therefore,it is necessary to establish reference intervals according to different sex. Using Hoffmann method,the reference intervals were TB male≤22.4 μmol/L,TB female≤18.5 μmol/L,DBil male≤6.4 μmol/L and DBil female≤5.1 μmol/L. The relative deviation between TB male,TB female and DBil male reference intervals and direct method reference interval was lower than reference change value（RCV）. The relative deviation between DBil female reference interval and direct method reference interval was higher than RCV. Conclusions The feasibility of using indirect method to establish The reference intervals of TB and DBil is verified.

Diagnostic value of maternal plasma D-dimer and hypertensive disorders of pregnancy in late pregnancy

CHEN Yiming, HUANG Jianxia, SHI Xiaochun, HE Pei, SHEN Yonghai

2020, 35(8):  753-756.  DOI: 10.3969/j.issn.1673-8640.2020.08.003

Asbtract ( 209 )   HTML ( 6)   PDF (928KB) ( 131 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the diagnostic value of maternal plasma D-dimer（DD） and hypertensive disorders of pregnancy（HDP） in the 28-32 gestational weeks. Methods The data of 2 299 pregnant women who delivered in Hangzhou Women's Hospital（Hangzhou Maternity and Child Health Care Hospital） from November 2014 to April 2019 were collected. There were 299 gestational hypertension（GH） patients,158 preeclampsia（PE） patients and 137 severe preeclampsia（SPE） patients. A total of 1 705 healthy pregnant women were enrolled as control group. The performance of DD for HDP was evaluated by receiver operating characteristic（ROC） curve. Results In GH,PE and SPE groups,the levels of DD were 1 730,1 950 and 2 030 μg/L,respectively,which were higher than that in control group（1 520 μg/L,P<0.001）. With progress,the DD levels increased gradually. For the diagnosis of GH,PE and SPE,the areas under curves（AUC） of DD were 0.560（0.525-0.595,P=0.001）,0.614（0.567-0.660,P<0.001） and 0.640（0.589-0.692,P<0.001）,respectively. When the cut-off values of DD were 1 325,1 875 and 1 795 μg/L,the sensitivities were 0.746,0.544 and 0.635,and the specificities were 0.389,0.652 and 0.624,respectively. Conclusions The levels of DD in the late trimester of pregnancy play a predictive role to HDP.

Clinical infection characteristics and their drug resistance genes of carbapenem-resistant Enterobacteriaceae in a hospital of Shandong

LU Shuhua, LI Xiaozhe, LIU Lingyun, JIN Chengqiang, GE Ningning, DONG Haixin

2020, 35(8):  757-762.  DOI: 10.3969/j.issn.1673-8640.2020.08.004

Asbtract ( 154 )   HTML ( 7)   PDF (1039KB) ( 97 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the clinical infection characteristics,drug susceptibility and drug resistance genotypes of carbapenem-resistant Enterobacteriaceae（CRE） in the Affiliated Hospital of Jining Medical University,and to provide a reference for the prevention and treatment of CRE infection and the management of multi-drug resistant bacteria. Methods Totally,93 isolates of CRE were collected,bacterial identification and drug susceptibility tests were carried out by VITEK2 Compact automatic microbial identification and drug susceptibility system,and the isolates were confirmed by automatic rapid microbial mass spectrometry. Carbapenemase was confirmed by simplified carbapenem inactivation method（sCIM） phenotype confirmation test. Carbapenemase models were determined and identified by Xpert Carba-R. Carbapenemase resistance gene was amplified by polymerase chain reaction（PCR） and sequenced. Results CRE isolates were mainly isolated from sputum,blood and urine samples. CRE isolates were mainly distributed in intensive care unit. CRE isolates were resistant to 24 commonly used antibiotics and sensitive to tigecycline. Totally,79 isolates were positive for carbapenemase phenotype by sCIM phenotype confirmation test. Xpert Carba-R showed that there were 38 isolates of KPC gene positive,31 isolates of NDM gene positive,6 isolates of IMP gene positive and 4 isolates of NDM+KPC gene positive. A total of 38 isolates carrying KPC resistance genes,31 isolates carrying NDM resistance genes,6 isolates carrying IMP resistance genes and 4 isolates carrying KPC + NDM resistance genes. VIM and OXA-48 resistance genes were not determined. Conclusions CRE in the Affiliated Hospital of Jining Medical University is resistant to many commonly used antibiotics,but it has good drug susceptibility to tigecycline in vitro. The carbapenem-resistant gene of Klebsiella pneumoniae is mainly KPC type,and that of Escherichia coli is mainly NDM type. The surveillance should be strengthened to prevent its outbreak and nosocomial epidemic.

Role of an elevated D-dimer cut-off value for the prognosis of lung cancer

LIU Qin, CHEN Chong, YUE Chao

2020, 35(8):  763-766.  DOI: 10.3969/j.issn.1673-8640.2020.08.005

Asbtract ( 139 )   HTML ( 8)   PDF (844KB) ( 98 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the role of an elevated D-dimer（DD） cut-off value for the prognosis of lung cancer. Methods The clinical and laboratory data of 153 patients with lung cancer were studied retrospectively. Carcinoembryonic antigen（CEA）,cytokeratin-19 fragment（CYFRA21-1）,lactate dehydrogenase（LDH）,squamous cell carcinoma antigen（SCC-Ag） and DD were determined before chemotherapy or surgery,and the results were analyzed statistically. Results There was no statistical significance for overall survival rate between different sex,age,CEA,CYFRA21-1,LDH and DD（P>0.05）. The overall survival rate in high DD（≥981 ng/mL） group was lower than that in normal DD（<981 ng/mL） group（P<0.05）. Conclusions The elevated DD cut-off value to 981 ng/mL is beneficial to prognosis prediction in lung cancer.

Correlations of NLR and PLR with hyperlipidemia

ZHANG Tao, LIU Qin, LIU Aiping, DING Yaodong

2020, 35(8):  767-770.  DOI: 10.3969/j.issn.1673-8640.2020.08.006

Asbtract ( 166 )   HTML ( 5)   PDF (848KB) ( 91 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the correlations of neutrophil-to-lymphocyte ratio（NLR） and platelet-to-lymphocyte ratio（PLR） with hyperlipidemia（HLP）. Methods The study included 495 patients with HLP,and their results of laboratory parameters were analyzed retrospectively. Totally,500 healthy subjects were enrolled as control group. Results HLP group had higher NLR and PLR compared to those of control group（P=0.007）. In the univariate analysis,white blood cell,hemoglobin,NLR and PLR were moderately correlated with HLP. Receiver operating characteristic（ROC） curve analysis showed that the optimal cut-off value for NLR was 1.71（P<0.001）. The sensitivity was 68.0%,and the specificity was 78.5%. The predictive strength of NLR was better than those of the other parameters. A strong positive correlation was revealed between the severity of HLP and NLR. Conclusions Patients with HLP have higher NLR and PLR compared to healthy subjects. NLR increases with the severity of HLP.

Distribution and drug resistance of bacteria in patients with acute exacerbation of chronic obstructive pulmonary disease

HUANG Junmin, ZHUANG Xucui, QIU Shuang

2020, 35(8):  771-774.  DOI: 10.3969/j.issn.1673-8640.2020.08.007

Asbtract ( 122 )   HTML ( 12)   PDF (828KB) ( 65 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the distribution and drug resistance of bacteria in patients with acute exacerbation of chronic obstructive pulmonary disease（AECOPD）,and to provide a reference for clinical rational use of drugs. Methods The positive results of sputum culturing and the results of antimicrobial susceptibility test of 107 patients with AECOPD from Beijing Shijitan Hospital of the Capital Medical University were analyzed retrospectively. Results A total of 139 isolates were isolated,58.3% of them were Gram-negative bacilli,25.2% of them were Gram-positive cocci,and 16.5% of them were fungi. Among the Gram-negative bacilli,the main pathogens included Klebsiella pneumoniae,Acinetobacter baumannii,Pseudomonas aeruginosa. Staphylococcus aureus and Streptococcus pneumoniae were the most common pathogens in Gram-positive cocci. Candida albicans was the most common pathogen in fungi. Klebsiella pneumoniae and Acinetobacter baumannii had the highest drug resistance to cephalosporin antibiotics,but were sensitive to β-lactamase inhibitor compounds. Acinetobacter baumannii was only susceptible to carbapenem and cefoperazone-sulbactam. The antibiotic resistance of Gram-positive cocci was serious,but vancomycin,linezolid and tigecycline were still effective. Conclusions Gram-negative bacilli are predominant pathogens in AECOPD patients from Beijing Shijitan Hospital of the Capital Medical University. The surveillance of bacterial resistance has significance to the clinical use of drugs.

Analysis of mixed infection with common respiratory tract pathogens in patients with corona virus disease 2019

CHANG Zhongyong, LIAO Guolin, WANG Qiang, YANG Weibin

2020, 35(8):  775-777.  DOI: 10.3969/j.issn.1673-8640.2020.08.008

Asbtract ( 222 )   HTML ( 4)   PDF (798KB) ( 63 )  

Figures and Tables | References | Related Articles | Metrics

Objective To understand the status of corona virus disease 2019（COVID-19） patients with respiratory tract pathogen mixed infection. Methods Totally,500 patients with COVID-19 were classified into 4 groups,including light group（205 cases）,common group（195 cases）,severe group（65 cases） and critical group（35 cases）. The IgM antibodies of 8 common respiratory tract pathogens in patients with COVID-19 were determined by indirect immunofluorescence,which included influenza A,influenza B,parainfluenza virus,respiratory syncytial virus,adenovirus,Mycoplasma pneumoniae,Chlamydia pneumoniae and Legionella pneumophila IgM. Results The respiratory tract pathogen mixed infection accounted for 9.76%,10.26%,18.46% and 28.57% in light group（20 cases）,common group（20 cases）,severe group（12 cases） and critical group（10 cases）,respectively（P<0.05）. The respiratory tract pathogen mixed infection totally accounted for 12.4%（62 cases）,of which 26 cases had multiple infections,accounting for 5.2%,showing an increasing trend（P<0.05）. Conclusions There are mixed infection in patients with COVID-19. The rate of mixed infection and the severity of COVID-19 are increasing. In the clinical diagnosis and treatment process,it should pay attention to the combined usage of drugs to improve the cure rate.

Analysis of laboratory determination data of 328 corona virus disease 2019 patients in Shanghai

WEI Jianhao, GUO Qian, LI Haicong, HAO Menglu, XU Sheng, QI Weiqiang, LING Yun, HAN Weijing, LIU Yili, ZHU Zhaoqin

2020, 35(8):  778-783.  DOI: 10.3969/j.issn.1673-8640.2020.08.009

Asbtract ( 498 )   HTML ( 9)   PDF (854KB) ( 136 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the roles of inflammatory indicators and cytokines in the occurrence and development of corona virus disease 2019（COVID-19）. Methods From January to February 2020,328 patients with COVID-19 in Shanghai Public Health Clinical Center were classified into 2 groups according to disease severity,including light/common group and severe/critical group. By retrospective analysis,the laboratory determination data of inflammatory indicators and cytokines,such as C-reactive protein（CRP）,erythrocyte sedimentation rate（ESR）,high-sensitivity C-reactive protein（hs-CRP）,heparin-binding protein（HBP）,lactate dehydrogenase（LDH）,procalcitonin（PCT）,white blood cell（WBC） count,lymphocyte（LPC） count,interleukin（IL）-1β,IL-2,IL-4,IL-5,IL-6,IL-8,IL-10,IL-12P70,IL-17,tumor necrosis factor alpha（TNF-α）,interferon alpha（IFN-α） and interferon gamma（INF-γ）,were collected. Results Compared with light/common group,severe/critical group had a higher age [68.5（11.5） years old,P<0.01],longer hospitalization time [21（10） d,P<0.01] and lower discharge rate（32.14%,P<0.01）. Compared with light/common group,in severe/critical group,the levels of CRP[55（84.5） mg/L,P<0.01],hs-CRP[43.58（76.46） mg/L,P<0.01],HBP[61.37（111.01） ng/mL,P<0.01],LDH[354.5（150.5） U/L,P<0.01],PCT[0.09（0.21） mg/L,P<0.01],IL-5[0.49（1.6） pg/mL,P=0.01],IL-6[10.78（56.24） pg/mL,P<0.01],IL-8[12.97（19.4） pg/mL,P<0.01] and IL-10[0.82（1.94） pg/mL,P=0.03] were increased,and LPC count [0.77（0.5）×10⁹/L,P<0.01],IL-4[0（0） pg/mL,P<0.01] and IL-17[0（0） pg/mL,P=0.01] were decreased. ConclusionsInflammatory indicators and cytokines between light/common group and severe/critical group among COVID-19 patients have obvious difference. The levels of CRP,HBP,hs-CRP and PCT indicate the development of COVID-19 to severe situation. The determinations of LPC,IL-4,IL-6,IL-8 and IL-10 levels play roles in predicting the clinical progression,especially the occurrence of inflammatory storm.

Value of circulating tumor DNA for the diagnosis of melanoma：a Meta-analysis

WAN Wenting, YU Bo, GONG Mengchun, SHI Wenzhao, GUO Hao, YANG Jie

2020, 35(8):  784-790.  DOI: 10.3969/j.issn.1673-8640.2020.08.010

Asbtract ( 162 )   HTML ( 2)   PDF (1184KB) ( 109 )  

Figures and Tables | References | Related Articles | Metrics

Objective To evaluate the diagnostic value of circulating tumor DNA（ctDNA） for melanoma by a Meta-analysis. Methods Medline,Excerpta Medica Database（Embase）,Cochrane Library database,CNKI database and VIP database were searched systematically to identify studies reporting the diagnostic value of ctDNA for melanoma,until January 2019. The heterogeneity was analyzed by I² and Q tests. The pooled sensitivity,specificity,positive likelihood ratio（PLR）,negative likelihood ratio（NLR）,diagnostic odds ratio（DOR） and the area under receiver operating characteristic（ROC） curve（AUC） were analyzed. Deek's funnel plots were used to evaluate publication bias. Results A total of 10 eligible studies involving 1 360 patients were included in this Meta-analysis. The pooled sensitivity,specificity,PLR and NLR were 0.71 [95% confidence interval（CI） 0.63-0.79],0.94（95%CI 0.91-0.96）,11.3（95%CI 7.2-17.5） and 0.31（95%CI 0.23-0.41）,respectively. The AUC and DOR were 0.94（95%CI 0.92-0.96）and 37（95%CI 19-71）. Deek's funnel plots showed a publication bias. Conclusions ctDNA is potential for the auxiliary diagnosis of melanoma.

Role of serum pepsinogen in the diagnosis of Helicobacter pylori infection

JIN Xin, LI Shujin, CHEN Zhen, SHEN Zuojun

2020, 35(8):  791-794.  DOI: 10.3969/j.issn.1673-8640.2020.08.011

Asbtract ( 207 )   HTML ( 8)   PDF (855KB) ( 112 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the diagnostic roles of pepsinogen Ⅰ（PGⅠ）,pepsinogen Ⅱ（PGⅡ） and pepsinogen Ⅰ/ pepsinogenⅡ ratio（PGR） for Helicobacter pylori（HP） infection. Methods A total of 88 inpatients who were examined by serum HP antibody and pepsinogen determinations and urea breath test were enrolled. The cases with positive results of both urea breath test and HP antibody determination were classified as HP infection group（56 cases）,and those with negative results were classified as HP non-infection group（32 cases）. Serum PGⅠand PGⅡ levels were determined,and PGR was calculated. Receiver operating characteristic（ROC） curve was used to evaluate their roles in the diagnosis of HP infection. Results The levels of PGⅠand PGⅡ were higher in HP infection group than those in HP non-infection group（P=0.021 and 0.000）,and PGR was lower（P=0.000）. The levels of PGⅠand PGⅡ were higher in males than those in females（P=0.002 and 0.008）,and there was no statistical significance in PGR between males and females（P=0.601）. ROC curve analysis showed that the areas under curves （AUC） of PGⅠ,PGⅡ and PGR for the diagnosis of HP infection were 0.648,0.785 and 0.789,respectively. The optimal cut-off value of PG Ⅱ was 15.57 μg/L,and that of PGR was 9.27. The sensitivities were 76.79% and 75.00%,and the specificities were 78.13% and 68.75%,respectively. The AUC of the combined determination of PGⅡ and PGR was 0.829,and the sensitivity and specificity were 92.86% and 65.63%,respectively. Conclusions PGⅡ and PGR play roles in the diagnosis of HP infection. If PGⅡ and PGR were combinedly determined,the diagnostic effect would be improved.

Clinical significance of detecting plasma EGFR T790M gene mutation in patients with NSCLC by 3D digital PCR

YUAN Shiyang, LIU Chuan, OUYANG Xiaochun, XIE Junping, HE Rongzhi, LI Lixiang, ZOU Yeqing

2020, 35(8):  806-810.  DOI: 10.3969/j.issn.1673-8640.2020.08.015

Asbtract ( 143 )   HTML ( 4)   PDF (1120KB) ( 122 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the clinical significance of a new 3D digital polymerase chain reaction（PCR） technology platform for detecting epidermal growth factor receptor（EGFR） T790M gene mutation in patients with non-small cell lung cancer（NSCLC）. Methods A total of 70 plasma samples from NSCLC patients with first-generation EGFR-tyrosine kinase inhibitor（TKI） resistance were collected. Detection of EGFR T790M gene mutation was performed by amplification refractory mutation system（ARMS） and 3D digital PCR. If the results were different,tissue wax samples in the same period were collected and validated by ARMS. Results Of the 70 plasma samples,EGFR T790M gene mutation was detected in 30 cases by ARMS and 34 cases by 3D digital PCR. Among the 4 plasma samples,3D digital PCR showed positive for EGFR T790M gene mutation,while ARMS showed wild type. Kappa consistency test and paired χ² test results showed that there was a good agreement between the 2 methods,and the Kappa value was 0.885（P<0.001）. Conclusions 3D digital PCR can be used to detect plasma EGFR T790M gene mutation in patient with NSCLC,and it can detect the cases missed by ARMS.

Establishment and performance evaluation of candidate reference measurement procedure for serum aldosterone by liquid chromatography-tandem mass spectrometry

ZOU Jihua, LI Quanle, SHEN Min, ZHANG Man, YANG Xiaodong, TU Minmin, FENG Dongfang, SUN Hongna

2020, 35(8):  811-817.  DOI: 10.3969/j.issn.1673-8640.2020.08.016

Asbtract ( 152 )   HTML ( 14)   PDF (867KB) ( 138 )  

Figures and Tables | References | Related Articles | Metrics

Objective To establish a candidate reference measurement procedure for serum aldosterone by isotope dilution liquid chromatography-tandem mass spectrometry（ID-LC-MS/MS）. Methods The sample was prepared by liquid-liquid extraction with methyltert-bytylether as extractant. The aldosterone-²H₇ was selected as internal standard. Samples were prepared by liquid-liquid extraction,and aldosterone was detected by ID-LC-MS/MS in negative ion mode. The basic analysis performance of the established candidate reference measurement procedure,including accuracy,precision,sensitivity and linearity,was evaluated. Results The linear range of aldosterone was 0.08-3.55 nmol/L. The limit of detection and the limit of quantitation were （LOQ）0.012 nmol/L and 0.045 nmol/L,respectively. The within-run coefficients of variation（CV） was <5.0%,and the between-run CV was <4.1%. The average recovery rates were 98.52%-100.14%. The bias of the International Federation of Clinical Chemistry and Laboratory Medicine External Quality Assessment Scheme for Reference Laboratories in Laboratory Medicine（RELA） was <1.6%. Conclusions The established ID-LC-MS/MS for detecting aldosterone in serum is accuracy and precise,and may be used as a candidate reference measurement procedure.

Influence of OLFM4 on BCG-induced apoptosis and intracellular survival rate in macrophage

WANG Qiyuan, JI Wenlan, YU Xiufeng

2020, 35(8):  818-822.  DOI: 10.3969/j.issn.1673-8640.2020.08.017

Asbtract ( 119 )   HTML ( 5)   PDF (1373KB) ( 116 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the role and mechanism of olfactomedin（OLFM4） in Bacillus Calmette-Guerin（BCG）-induced macrophage RAW264.7 apoptosis and BCG survival rate. Methods The mRNA expression of OLFM4 was determined by reverse transcription-polymerase chain reaction（PCR）. Western blotting was conducted to determine the expression level of OLFM4 and apoptosis-associated protein Bcl-2 and Bax. The survival rate of BCG was determined by colony-forming unit. Enzyme-linked immunosorbent assay was performed to analyze cell apoptosis rate. Results The expression of OLFM4 at both mRNA and protein levels was dramatically elevated in a time dependent manner in macrophage RAW264.7 cells stimulated with BCG. The depletion of OLFM4 apparently repressed BCG-induced macrophage apoptosis. Meanwhile,the knockdown of OLFM4 enhanced strongly the expression of anti-apoptotic Bcl-2 concomitant with reducing the expression of pro-apoptotic protein Bax in RAW264.7 cells followed by BCG infection. Moreover,the silencing of OLFM4 enhanced obviously the intracellular survival of BCG during the infection of macrophages with BCG. Mechanically,the deletion of OLFM4 blocked TLR2/NF-κB signaling pathway through inhibiting the expression of p-p65 and TLR2. Conclusions The silencing of OLFM4 represses BCG-induced macrophage apoptosis and increases BCG survival rate in macrophage RAW264.7 through blocking TLR2/NF-κB signaling pathway. The underlying mechanism of OLFM4 in host cells on BCG infection warrants further study for the development of novel agents for clinical treatment of tuberculosis （TB）.

Similarities and differences of limits of detection in health institutions and pharmaceutical institutions

ZHU Lingfeng, GE Danhong, FANG Huiling, ZHU Yuqing

2020, 35(8):  825-828.  DOI: 10.3969/j.issn.1673-8640.2020.08.019

Asbtract ( 195 )   HTML ( 6)   PDF (804KB) ( 254 )  

Figures and Tables | References | Related Articles | Metrics

Clinical laboratories should complete the performance verification of the limit of detection（LoD） of qualitative test items,when the manufacturer of in vitro diagnostic reagents only declares the limit of blank（LoB） according to the standards of pharmaceutical institutions. The definitions of LoD and LoB in several medical related institution standards are analyzed,and the similarities and differences are pointed out. C50 concentration and C95 concentration in qualitative test items are correlated with LoB and LoD in quantitative test items.

Research progress of Pseudomonas aeruginosa transcription factor PsrA

LI Chanchan, WANG Ru, LUO Wenying

2020, 35(8):  832-836.  DOI: 10.3969/j.issn.1673-8640.2020.08.021

Asbtract ( 116 )   HTML ( 4)   PDF (878KB) ( 168 )  

References | Related Articles | Metrics

Pseudomonas aeruginosa （PA） is a class of opportunistic pathogens,and the pathogenesis is still not fully understood. Its resistance has always been a clinical problem. Biofilm has always been the focus of the study. This review discusses the research results and the significance of PA PsrA,in order to provide new ideas for its clinical diagnosis and treatment.

Role of circular RNA in tumor proliferation and metastasis

HOU Yanyan, FAN Jinong, LIU Xiangfan, NI Peihua

2020, 35(8):  837-842.  DOI: 10.3969/j.issn.1673-8640.2020.08.022

Asbtract ( 122 )   HTML ( 5)   PDF (823KB) ( 122 )  

Figures and Tables | References | Related Articles | Metrics

Circular RNA（circDNA） is a new type of endogenous non-coding RNA,which is widely expressed in eukaryotes,and it has species,tissue and cell specificities. It plays a role in the development of tumors. This review mainly discusses the role of circRNA in tumor growth and metastasis,which makes a prospect of its application in diagnosis and treatment.

Application of clinical laboratory testing techniques in the diagnosis of COVID-19

DU Jinghui, LI Sha, LIU Xu

2020, 35(8):  843-848.  DOI: 10.3969/j.issn.1673-8640.2020.08.023

Asbtract ( 150 )   HTML ( 8)   PDF (815KB) ( 78 )  

Figures and Tables | References | Related Articles | Metrics

Corona virus disease 2019（COVID-19） at the end of 2019 poses a serious and immediate threat to national and global public health. For its diagnosis and treatment,clinical laboratory testing has important reference value,and the nucleic acid detection for severe acute respiratory syndrome coronavirus 2（SARS-CoV-2） is the gold standard in the diagnosis of COVID-19. This review focuses on clinical laboratory testing techniques in the definite diagnosis,differential diagnosis and auxiliary diagnosis of COVID-19,in order to provide clinicians and laboratory workers with guidance on the clinical laboratory testing techniques for the diagnosis of COVID-19.