Influence of OLFM4 on BCG-induced apoptosis and intracellular survival rate in macrophage

doi:10.3969/j.issn.1673-8640.2020.08.017

Abstract

Abstract:

Objective To investigate the role and mechanism of olfactomedin（OLFM4） in Bacillus Calmette-Guerin（BCG）-induced macrophage RAW264.7 apoptosis and BCG survival rate. Methods The mRNA expression of OLFM4 was determined by reverse transcription-polymerase chain reaction（PCR）. Western blotting was conducted to determine the expression level of OLFM4 and apoptosis-associated protein Bcl-2 and Bax. The survival rate of BCG was determined by colony-forming unit. Enzyme-linked immunosorbent assay was performed to analyze cell apoptosis rate. Results The expression of OLFM4 at both mRNA and protein levels was dramatically elevated in a time dependent manner in macrophage RAW264.7 cells stimulated with BCG. The depletion of OLFM4 apparently repressed BCG-induced macrophage apoptosis. Meanwhile,the knockdown of OLFM4 enhanced strongly the expression of anti-apoptotic Bcl-2 concomitant with reducing the expression of pro-apoptotic protein Bax in RAW264.7 cells followed by BCG infection. Moreover,the silencing of OLFM4 enhanced obviously the intracellular survival of BCG during the infection of macrophages with BCG. Mechanically,the deletion of OLFM4 blocked TLR2/NF-κB signaling pathway through inhibiting the expression of p-p65 and TLR2. Conclusions The silencing of OLFM4 represses BCG-induced macrophage apoptosis and increases BCG survival rate in macrophage RAW264.7 through blocking TLR2/NF-κB signaling pathway. The underlying mechanism of OLFM4 in host cells on BCG infection warrants further study for the development of novel agents for clinical treatment of tuberculosis （TB）.

Key words: Olfactomedin, Macrophage, Apoptosis, Mycobacterium tuberculosis

CLC Number:

R446.1

WANG Qiyuan, JI Wenlan, YU Xiufeng. Influence of OLFM4 on BCG-induced apoptosis and intracellular survival rate in macrophage[J]. Laboratory Medicine, 2020, 35(8): 818-822.

Figures/Tables 4

References 14

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