Bronchoscopy combined with sputum Mycobacterium tuberculosis ribonucleic acid and serum Mycobacterium tuberculosis specific antibody determinations for diagnosis of negative pulmonary tuberculosis

doi:10.3969/j.issn.1673-8640.2023.09.011

Abstract

Abstract:

Objective To investigate the efficiency of bronchoscopy combined with sputum Mycobacterium tuberculosis RNA（TB-RNA） and serum Mycobacterium tuberculosis specific antigen（TB-SA） determinations for the diagnosis of negative pulmonary tuberculosis. Methods A total of 104 patients with bacterial-negative pulmonary tuberculosis diagnosed by sputum Mycobacterium tuberculosis（MTB） culture in Huai'an Fourth People's Hospital from October 2018 to October 2019 were enrolled as observation group，and 80 patients with non-pulmonary tuberculosis during the same period were enrolled as control group. Bronchoscopy was performed，sputum samples were collected to determine sputum TB-RNA and serum TB-SA in sputum samples，and the diagnostic efficiency of each determination method for bacterial-negative pulmonary tuberculosis was evaluated. Results The positive rate of bronchoscopy in observation group（62.50%） was higher than that in control group（32.0%）（P<0.05）. The positive rate of sputum TB-RNA in observation group（78.85%） was higher than that in control group（22.50 %）（P<0.05）. The positive rate of serum TB-SA in observation group （68.27%）was higher than that in control group（20.00%）（P<0.05）. The sensitivity of the combined determination of the 3 methods was higher than those of bronchoscopy（P<0.001），TB-RNA determination（P=0.037） and TB-SA determination（P<0.001）. The accuracy of the combined determination of the 3 methods was higher than those of bronchoscopy（P<0.001），TB-RNA determination（P=0.028） and TB-SA determination（P<0.001）. There was no statistical significance in the specificity of the combined determination of the 3 methods（P>0.05）. Conclusions Bronchoscopy combined with sputum TB-RNA and serum TB-SA can effectively improve the sensitivity for the diagnosis of bacterial-negative pulmonary tuberculosis，which provides a determination method for the clinical diagnosis of bacterial-negative pulmonary tuberculosis，with high clinical application value.

Key words: Bronchoscopy, Mycobacterium tuberculosis RNA, Mycobacterium tuberculosis specific antibody, Bacterial-negative pulmonary tuberculosis, Diagnosis

CLC Number:

R378.91

SUN Xinlin, YE Xingming, LI Qian, ZHAO Lijun. Bronchoscopy combined with sputum Mycobacterium tuberculosis ribonucleic acid and serum Mycobacterium tuberculosis specific antibody determinations for diagnosis of negative pulmonary tuberculosis[J]. Laboratory Medicine, 2023, 38(9): 870-873.

Figures/Tables 4

References 20

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组别	例数	阳性/[例（%）]	阴性/[例（%）]
观察组	104	65（62.50）	39（37.50）
对照组	80	26（32.50）	54（67.50）
χ²值		16.280
P值		<0.001

组别	例数	阳性/[例（%）]	阴性/[例（%）]
观察组	104	65（62.50）	39（37.50）
对照组	80	26（32.50）	54（67.50）
χ²值		16.280
P值		<0.001

组别	例数	阳性/[例（%）]	阴性/[例（%）]
观察组	104	82（78.85）	22（21.15）
对照组	80	18（22.50）	62（77.50）
χ²值		57.862
P值		<0.001

组别	例数	阳性/[例（%）]	阴性/[例（%）]
观察组	104	82（78.85）	22（21.15）
对照组	80	18（22.50）	62（77.50）
χ²值		57.862
P值		<0.001

组别	例数	阳性/[例（%）]	阴性/[例（%）]
观察组	104	71（68.27）	33（31.73）
对照组	80	16（20.00）	64（80.00）
χ²值		42.266
P值		<0.001