Correlation between total IgE levels in cord blood and AICDA gene polymorphism

doi:10.3969/j.issn.1673-8640.2014.02.016

Abstract

Abstract: Objective To investigate the correlation between total immunoglobulin E(IgE) levels in cord blood and activation-induced cytidine deaminase(AICDA) gene polymorphism. Methods Cord blood samples of 70 infants whose mothers had allergic diseases and 97 infants whose mothers had no history of allergic diseases were collected. Genomic DNA were extracted from cord blood samples, and polymerase chain reaction-ligase detection reaction (PCR-LDR) was used to detect rs2518144, rs2028373, rs11046349 sites in AICDA gene. Meantime, total IgE levels in cord blood were detectedby electro-chemiluminescence immunoassay (ECLIA).Results There was no significant difference in distribution and allelic distribution frequencies of 3 rs2518144 genotypes (AA/AG/GG) and 3 rs11046349genotypes (TT/TG/GG) between allergic disease group and non-allergic disease group(P＞0.05).However, significant difference in genotype distribution and allelic distribution frequencies of 3 rs2028373genotypes (AA/AG/GG) between the 2 groups was detected(P=0.020 and 0.006). The genotyps of rs2518144, rs2028373 and rs11046349 sites were not related with total IgE levels(P＞0.05). Conclusions rs2028373 gene polymorphism is significantly correlated with allergic diseases, but the genotypes of rs2518144, rs2028373 and rs11046349 sites are not correlated with total IgE levels.

Key words: Activation-induced cytidine deaminase, Immunoglobulin E, Gene polymorphism, Cord blood

CLC Number:

Q503

TANG Lin, LI Jia, DAI Huirong, LI Li.. Correlation between total IgE levels in cord blood and AICDA gene polymorphism[J]. , 2014, 29(2): 158-161.

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