Abstract:

Objective To investigate the relationship between the polymorphism of methylene tetrahydrofolate reductase（MTHFR） gene C677T polymorphism,blood lipid levels and gestational diabetes mellitus（GDM）. Methods Totally,144 patients with GDM and 142 healthy pregnant women were enrolled as case group and control group,respectively. The polymorphism of MTHFR gene C677T and blood lipid levels [total cholesterol（TC）,triglyceride（TG）,low-density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C）] were determined,and non- high-density lipoprotein cholesterol（non-HDL-C） levels were calculated. Results There was statistical significance in genotype distribution and allele frequency of MTHFR gene in GDM group compared with control group（P<0.01）. Compared with control group,the levels of TG,TC,LDL-C,non-HLD-C in GDM group were increased（P<0.05）,and the HDL-C level was decreased（P<0.01）. Compared with CC genotype GDM patients,TT genotype GDM patients had higher TC and non-HDL-C levels（P<0.05） and lower HDL-C levels（P<0.01）. The levels of TG,TC,LDL-C and non-HDL-C in GDM patients with CT genotype were increased（P<0.05）,while HDL-C was decreased（P<0.01）. Compared with CC genotype,TG,TC and non-HDL-C levels were increased in healthy pregnant women with TT and CT genotype. T allele frequency,LDL-C levels and non-HDL-C levels were positively correlated with GDM [odds ratio（OR）=5.25,95% confidence interval 3.66-7.53;OR=2.44,95% confidence interval 1.69-3.53;OR=1.65,95%confidence interval 1.41-1.92]. HDL-C was negatively correlated with GDM（OR=0.05,95% confidence interval 0.02-0.13）. Conclusions The polymorphism of MTHFR gene C677T may be related tso the abnormality of blood lipid in GDM,and increased MTHFR T allele frequency may be a risk factor for GDM.

Key words: Methylene tetrahydrofolate reductase, Gene polymorphism, Gestational diabetes mellitus