Correlation between TSLP gene polymorphism and toddler atopic dermatitis

doi:10.3969/j.issn.1673-8640.2019.03.014

Abstract

Abstract:

Objective To investigate the correlation between thymic stromal lymphopoietin （TSLP）gene polymorphism in cord blood and toddler atopic dermatitis （AD）. Methods A total of 393 pregnant women in a prospective birth cohort were enrolled. Genomic DNA was extracted from cord blood samples,and polymerase chain reaction （PCR）- ligase detection reaction（LDR） was used to detect rs3806933,rs1545169,rs764917,rs1837253,rs2289276 and rs11466749 mutations in TSLP gene. Totally,285 toddlers received a phone call questionnaire at the age of 2 to determine whether they had AD or not,which was judged by pediatricians after birth, and 162 cases were diagnosed AD. Results There was no statistical significance in the genotype distribution frequencies of rs3806933,rs1545169,rs764917,rs1837253 and rs2289276 between AD group and non-AD group （P>0.05）. There was statistical significance in the genotype distribution frequency of rs11466749 （AA/AG）（AD group：rs11466749 locus 60.0% AA and 34.3% AG;non-AD group：rs11466749 locus 40.0% AA and 65.7% AG. Conclusions The gene polymorphism of TSLP gene rs11466749 in cord blood is correlated with AD.

Key words: Atopic dermatitis, Gene polymorphism, Cord blood

CLC Number:

TANG Lin, ZHU Chenchen, XIAO Ye, WEI Bing, CAI Mei, ZHANG Xiaojing, TANG Zhenhua. Correlation between TSLP gene polymorphism and toddler atopic dermatitis[J]. Laboratory Medicine, 2019, 34(3): 249-252.

Figures/Tables 6

References 13

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组别	例数	基因型			等位基因
组别	例数	CC	CT	TT	C	T
无AD组	123	44（39.6）	60（43.5）	19（52.8）	148（60.2）	98（39.8）
有AD组	162	67（60.4）	78（56.5）	17（56.8）	212（65.4）	112（34.6）

组别	例数	基因型			等位基因
组别	例数	CC	CT	TT	C	T
无AD组	123	44（39.6）	60（43.5）	19（52.8）	148（60.2）	98（39.8）
有AD组	162	67（60.4）	78（56.5）	17（56.8）	212（65.4）	112（34.6）

组别	例数	基因型			等位基因
组别	例数	TT	GT	GG	G	T
无AD组	123	47（42.3）	60（42.0）	16（51.6）	91（37.0）	155（63.0）
有AD组	162	64（57.7）	83（58.0）	15（48.4）	113（34.9）	211（65.1）

组别	例数	基因型			等位基因
组别	例数	TT	GT	GG	G	T
无AD组	123	47（42.3）	60（42.0）	16（51.6）	91（37.0）	155（63.0）
有AD组	162	64（57.7）	83（58.0）	15（48.4）	113（34.9）	211（65.1）

组别	例数	基因型			等位基因
组别	例数	AA	AC	CC	A	C
无AD组	123	61（44.9）	54（43.2）	8（33.3）	175（71.1）	71（28.9）
有AD组	162	75（55.1）	71（56.8）	16（66.7）	221（68.2）	103（31.8）