Correlations between IL-13,AICDA and TSLP gene loci and IgE in cord blood

doi:10.3969/j.issn.1673-8640.2019.06.009

Abstract

Abstract:

Objective To investigate the correlations between interleukin 13（IL-13）,activation-induced cytidine deaminase（AICDA） and thymic stromal lymphopoietin（TSLP） gene loci and immunoglobulin E（IgE） in cord blood. Methods Cord blood samples were collected from 779 neonates. Genomic DNA was extracted from cord blood samples,and polymerase chain reaction-ligase detection reaction（PCR-LDR） was used to detect IL-13 gene loci（rs20541,rs1800925,rs848,rs1295686 and rs2069743）,AICDA gene loci（rs2518144,rs17792729,rs2580873,rs2028373 and rs7304723） and TSLP gene loci（rs3806933,rs1545169,rs764917,rs1837253,rs2289276 and rs11466749）. Cord blood IgE level was determined. Results There were correlations between IL-13 gene locus rs1295686（β=0.087,P=0.02）,AICDA gene locus rs2028373（β=-0.082,P=0.02）,TSLP gene loci rs2289276,rs764917 and rs1837253（β=-0.126,-0.083 and 0.188,P=0.01,0.03 and 0.00,respectively） and cord blood IgE. Conclusions IL-13 gene locus rs1295686,AICDA gene locus rs2028373 and TSLP gene loci rs2289276,rs764917 and rs1837253 may be allergy susceptible loci.

Key words: Interleukin 13 gene, Activation-induced cytidine deaminase gene, Thymic stromal lymphopoietin gene, Immunoglobulin E, Cord blood, Allergic disease

CLC Number:

R446.1

TANG Lin, ZHU Chenchen, ZHANG Xiaojing, XIAO Ye, WEI Bing, CAI Mei, TANG Zhenhua. Correlations between IL-13,AICDA and TSLP gene loci and IgE in cord blood[J]. Laboratory Medicine, 2019, 34(6): 522-525.

Figures/Tables 2

References 24

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引物	上游引物序列（5'~3'）	下游引物序列（5'~3'）	长度（bp）
IL-13基因
rs20541	CCAGTTTGTAAAGGACCTGC	TGATGCTTTCGAAGTTTCAG	100
rs1800925	GGGTTTCTGGAGGACTTCTA	TGCAGCCATGTCGCCTTTTC	80
rs848	CAGTACTTATTACCAGGGAC	TTGGAGCCAAGGGTTCAGAG	97
rs1295686	AACTGCTGCAGGACAAAGAG	TGGAAAGCCCCTGGTTTGTG	100
rs2069743	CACTGTGAGAGGGATTGTCA	CCCAGAAAGACCTCTGAATC	99
AICDA基因
rs2518144	GCACTCACAGTTGACCATTA	TGGACTTTGGTTATCTTCGC	97
rs17792729	GCTGATGACTTGGGATTAC	GGGACTGAGGAACAAGTTTA	100
rs2580873	ATCAAGAGTCCCCATTGCAG	CAGGCATCACTTGTGCTTTC	99
rs2028373	ACTGAATTTTCATGCAGCCC	CTGCTGGAATACTTTTGTAG	92
rs7304723	AAGCCATCCTCCCTCCTCAA	TGATAAAGCTGCGAACCGTG	92
TSLP基因
rs3806933	TCAATTTCAGGAAAAACGCC	AGTGCTAGAGGACTCTTCAG	90
rs1545169	CTTACAGTTCTGAATGCTGG	AAGAGAGCCTTCACCAGGAC	89
rs764917	TGTAAAATCGCAAAGTAGG	GAACAGTTATTCCAAAAATGC	109
rs1837253	GGTTACTTTGTAAAAGATCC	CCTACTGGACTCTATTGTG	100
rs2289276	TGGCAATGAGAGGCAAAACC	CCACGAGTGTAAGAGATCTG	95
rs11466749	AGGGCCAACAGGTATAAAGG	GGGACATTATCTTCCTTCAG	101

引物	上游引物序列（5'~3'）	下游引物序列（5'~3'）	长度（bp）
IL-13基因
rs20541	CCAGTTTGTAAAGGACCTGC	TGATGCTTTCGAAGTTTCAG	100
rs1800925	GGGTTTCTGGAGGACTTCTA	TGCAGCCATGTCGCCTTTTC	80
rs848	CAGTACTTATTACCAGGGAC	TTGGAGCCAAGGGTTCAGAG	97
rs1295686	AACTGCTGCAGGACAAAGAG	TGGAAAGCCCCTGGTTTGTG	100
rs2069743	CACTGTGAGAGGGATTGTCA	CCCAGAAAGACCTCTGAATC	99
AICDA基因
rs2518144	GCACTCACAGTTGACCATTA	TGGACTTTGGTTATCTTCGC	97
rs17792729	GCTGATGACTTGGGATTAC	GGGACTGAGGAACAAGTTTA	100
rs2580873	ATCAAGAGTCCCCATTGCAG	CAGGCATCACTTGTGCTTTC	99
rs2028373	ACTGAATTTTCATGCAGCCC	CTGCTGGAATACTTTTGTAG	92
rs7304723	AAGCCATCCTCCCTCCTCAA	TGATAAAGCTGCGAACCGTG	92
TSLP基因
rs3806933	TCAATTTCAGGAAAAACGCC	AGTGCTAGAGGACTCTTCAG	90
rs1545169	CTTACAGTTCTGAATGCTGG	AAGAGAGCCTTCACCAGGAC	89
rs764917	TGTAAAATCGCAAAGTAGG	GAACAGTTATTCCAAAAATGC	109
rs1837253	GGTTACTTTGTAAAAGATCC	CCTACTGGACTCTATTGTG	100
rs2289276	TGGCAATGAGAGGCAAAACC	CCACGAGTGTAAGAGATCTG	95
rs11466749	AGGGCCAACAGGTATAAAGG	GGGACATTATCTTCCTTCAG	101

基因位点	β值	P值
IL-13基因
rs20541	0.150	0.76
rs1800925	-0.010	0.92
rs848	-0.340	0.36
rs1295686	0.087	0.02
rs2069743	0.460	0.43
AICDA基因
rs2518144	0.050	0.56
rs17792729	-0.140	0.37
rs2580873	0.170	0.26
rs2028373	-0.082	0.02
rs7304723	-0.270	0.55
TSLP基因
rs3806933	-0.040	0.89
rs1545169	-0.140	0.19
rs764917	-0.083	0.03
rs1837253	0.188	0.00
rs2289276	-0.126	0.01
rs11466749	0.060	0.76

基因位点	β值	P值
IL-13基因
rs20541	0.150	0.76
rs1800925	-0.010	0.92
rs848	-0.340	0.36
rs1295686	0.087	0.02
rs2069743	0.460	0.43
AICDA基因
rs2518144	0.050	0.56
rs17792729	-0.140	0.37
rs2580873	0.170	0.26
rs2028373	-0.082	0.02
rs7304723	-0.270	0.55
TSLP基因
rs3806933	-0.040	0.89
rs1545169	-0.140	0.19
rs764917	-0.083	0.03
rs1837253	0.188	0.00
rs2289276	-0.126	0.01
rs11466749	0.060	0.76