Abstract: Objective　To compare the anti-interference abilities of immune turbidimetry and immune nephelometry for detecting specific proteins [immunoglobulin G（IgG），immunoglobulin M（IgM） and C reactive protein（CRP）]through the interferers of free bilirubin（FBil），conjugated bilirubin（CBil），hemoglobin（Hb） and chyle（CH）.   Methods　A total of 6 mixed serum samples were prepared under 2 levels（high/low） of specific proteins（IgG：8-12 g/L，＞16 g/L；IgM：1.0-1.5 g/L，＞2.0 g/L；CRP：4-10 mg/L，＞100mg/L）. The 4 interferers were prepared into 5 different concentrations（FBil：656，1 312，1 968，2 624 and 3 280 μmol/L；CBil：688，1 376，2 064，2 752 and 3 440 μmol/L；Hb：9.9，19.8，29.8，39.7 and 49.6 μmol/L；CH：3 000，6 000，9 000，12 000 and 15 000 FTU） by rules（1∶5，2∶5，3∶5，4∶5 and 5∶5），and the interferers were added to the mixed serum. The concentration of the specific proteins was detected by immune turbidimetry and immune nephelometry respectively，and the interference rate was calculated. The anti-interference abilities of the 2 methods were calculated according to Clinical and Laboratory Standards Institute（CLSI） EP7-A standard. The average concentration of the specific proteins with different interferers ±5% of the control tubes at the same concentration was considered to have interference. Results　By the immune nephelometry，the values to detect IgM（high level ）and CRP（ low level） with the interferer of Hb（9.9 and 29.8 μmol/L）and Hb（9.9，29.8 and 39.7 μmol/L） respectively，also the values to detect IgM（high level and low level ） with the interferer of CH（9 000，12 000 and 15 000 FTU）and CH（6 000，9 000，12 000 and 15 000 FTU） were all considered to have interference. However，by the immune turbidimetry，the values to detect IgG，IgM and CRP with the 4 interferers were considered to have no interference. Conclusions　To 4 interferers（FBil，CBil，Hb and CH），the anti-interference ability of immune turbidimetry is better than that of immune nephelometry. The immune turbidimetry shows better accuracy to detect specimens with the interferers of lipidemia and haemolysis as large particles in the clinical application.

Key words: Immunoglobulin G, Immunoglobulin M, C reactive protein, Immune turbidimetry, Immune nephelometry, Anti-interference ability