Abstract: Objective　To investigate the comparability of the results of alpha fetoprotein（AFP） and carcinoembryonic antigen（CEA） measured by light initiated chemiluminescence（LiCA） and enzyme-enhanced chemiluminescence，and evaluate the clinical performances of LiCA AFP and CEA kits.   Methods　According to the documents of National Committee for Clinical Laboratory Standards（NCCLS） and In Vitro Diagnostic Products Clinic Research Guideline，the analytical sensitivity，imprecision and linearity of LiCA AFP and CEA kits were evaluated，and 406 AFP serum samples and 442 CEA serum samples were detected by LiCA and enzyme-enhanced chemiluminescence respectively. The data were analyzed statistically. Results　The analytical sensitivities of AFP and CEA by LiCA were 0.112 and 0.05 ng/mL. The within-run and between-run coefficients of variation（CV） of AFP and CEA by LiCA were 4.32%，4.56% and 5.89%，6.47% respectively. The linearities of AFP and CEA by LiCA were 2.6-950.2 and 1.2-862.6 ng/mL. The correlation coefficients（r） of 2 chemiluminescence systems for AFP and CEA were ＞0.99. Conclusions　The clinical performances of LiCA AFP and CEA are satisfied to clinical measurement. There is a correlation between the 2 systems for AFP and CEA.

Key words: Light initiated chemiluminescence, Enzyme-enhanced chemiluminescence, Alpha fetoprotein, Carcinoembryonic antigen, Performance evaluation, Comparison