Abstract:

Objective To establish a fresh blood comparison program so as to ensure the comparable and consistent results of clinical specimens,and to improve the daily internal quality control（IQC） of hematology analyzers. Methods After the completion of daily IQC with supporting quality control products,2 mL fresh blood sample was drawn with test results within reference ranges. A hematology analyzer with good performance was selected as the target,and the remaining instruments were used as comparison instruments. The results tested by the target instrument were used as the target values,while the results tested by the comparison instruments were used as the measured values. Comparing the target values and the measured values,the relative deviations of white blood cell（WBC）,red blood cell（RBC）,hemoglobin（Hb）,hematocrit（HCT） and platelet（PLT） were calculated. The 1/2 of allowable error in the Clinical Laboratory Improvement Amendments of 1988（CLIA'88） was the relative deviation standard between different instruments,while the 1/3 of allowable error was the relative deviation standard of the same hematology analyzer at different times. The relative deviation was evaluated according to the relative deviation standard. Results The relative deviations of the comparison data among the comparison instruments and the reference instrument met the requirements of 1/2 CLIA'88 deviation range,showing the accurate and comparable determination results among the instruments. The relative deviations of the comparison data during the operation of the whole cell analyzers met the requirements of the 1/3 CLIA'88 deviation range,indicating the stable instruments. Conclusions The fresh blood comparison program could not only ensure the comparability of determination results among hematology analyzers and the stability of the same instrument during operation,but also perform convenient,economical and effective IQC on the hematology analyzers.

Key words: Hematology analyzer, Fresh blood, Comparison program, Internal quality control