基于cfDNA的NGS技术探究术后脓毒症患者血液微生物群特征

doi:10.3969/j.issn.1673-8640.2023.08.009

摘要/Abstract

摘要：

目的分析外科手术术后脓毒症患者血液微生物群特征。方法从SRA-NCBI公共数据库下载239例外科手术术后脓毒症患者（SP）和34例术后未感染患者[对照患者（CP）]血浆样本中细胞游离DNA（cfDNA）的原始测序数据，采用生物信息学方法进行再次分析。结果与CP比较，SP血液样本微生物多样性显著下降。SP血液样本中检测到12类显著富集的物种，其中相对丰度较高的代表性菌种有6类：麦芽寡养单胞菌、红串红球菌、食酸菌sp. KKS102、皮氏罗尔斯顿菌、屎肠球菌和人乙型疱疹病毒5。结论外科手术术后SP血液内微生物组成改变明显，有6类代表性物种可能在脓毒症的发展过程中起重要作用。

关键词: 脓毒症, 术后并发症, 细胞游离DNA, 微生物群

Abstract:

Objective To analyze the characteristic of blood microbiota in patients with sepsis after operation. Methods The sequencing data of microbial cell-free DNA（cfDNA） from plasma of 239 postoperative sepsis patients（SP）（SP group） and 34 postoperative patients without SP [control patients（CP）（CP group）] were downloaded from SRA-NCBI databases and reanalyzed by bioinformatics. Results The blood microbiota in SP group showed a decreased microbial diversity compared with CP group，which mainly enriched in 12 taxa. The most representative taxa which were increased in SP group，with higher relative abundance，included Stenotrophomonas maltophilia，Rhodococcus erythropolis，Acidovorax sp. KKS102，Ralstonia pickettii，Enterococcus faecium and human beta herpes virus 5. Conclusions The composition of blood microbiota is altered in SP group compared with CP group，and the 6 representative taxa may play roles in the development of SP.

Key words: Sepsis, Postoperative complication, Cell-free DNA, Microbial composition

中图分类号:

R446.1

梁栋, 梅月, 王腾蛟, 喻东. 基于cfDNA的NGS技术探究术后脓毒症患者血液微生物群特征[J]. 检验医学, 2023, 38(8): 753-759.

LIANG Dong, MEI Yue, WANG Tengjiao, YU Dong. Characteristic of blood microbiota of postoperative sepsis based on cfDNA NGS[J]. Laboratory Medicine, 2023, 38(8): 753-759.

图/表 6

参考文献 40

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微生物名称	CP/%	SP/%	P值
革兰阴性菌
麦芽寡养单胞菌	14.590	48.810	1.01×10^-60
食酸菌sp. KKS102	0.470	2.900	1.04×10^-56
皮氏罗尔斯顿菌	0.930	2.540	1.16×10^-35
屎肠球菌	0.002	2.530	1.92×10^-3
红串红球菌	0.053	14.170	1.71×10^-59
人乙型疱疹病毒5	<0.001	1.490	0.014

微生物名称	CP/%	SP/%	P值
革兰阴性菌
麦芽寡养单胞菌	14.590	48.810	1.01×10^-60
食酸菌sp. KKS102	0.470	2.900	1.04×10^-56
皮氏罗尔斯顿菌	0.930	2.540	1.16×10^-35
屎肠球菌	0.002	2.530	1.92×10^-3
红串红球菌	0.053	14.170	1.71×10^-59
人乙型疱疹病毒5	<0.001	1.490	0.014

微生物名称	血培养	NGS	本研究
前期研究^[15-16]
光滑念珠菌	+
鼠李糖乳酸杆菌	+		+
副干酪乳杆菌	+		+
经黏液真杆菌.	+		+
金黄色葡萄球菌	+		+
阴沟肠杆菌	+		+
普通拟杆菌		+	+
肺炎克雷伯菌		+	+
肠道沙门菌		+	+
假单胞菌属门多萨菌		+	+
多形拟杆菌		+	+
大肠埃希菌	+	+	+
白念珠菌	+	+
脆弱拟杆菌	+	+	+
本研究
屎肠球菌	+	+	+
人乙型疱疹病毒5		+	+
麦芽寡养单胞菌			+
食酸菌sp. KKS102			+
红串红球菌			+
皮氏罗尔斯顿菌			+

微生物名称	血培养	NGS	本研究
前期研究^[15-16]
光滑念珠菌	+
鼠李糖乳酸杆菌	+		+
副干酪乳杆菌	+		+
经黏液真杆菌.	+		+
金黄色葡萄球菌	+		+
阴沟肠杆菌	+		+
普通拟杆菌		+	+
肺炎克雷伯菌		+	+
肠道沙门菌		+	+
假单胞菌属门多萨菌		+	+
多形拟杆菌		+	+
大肠埃希菌	+	+	+
白念珠菌	+	+
脆弱拟杆菌	+	+	+
本研究
屎肠球菌	+	+	+
人乙型疱疹病毒5		+	+
麦芽寡养单胞菌			+
食酸菌sp. KKS102			+
红串红球菌			+
皮氏罗尔斯顿菌			+