微小RNA-132和高迁移率族蛋白1在脓毒症患儿外周血单个核细胞中的表达及临床意义

doi:10.3969/j.issn.1673-8640.2018.09.010

摘要/Abstract

摘要：

目的探讨微小RNA-132（miR-132）和高迁移率族蛋白1（HMGB1）在脓毒症患儿外周血单个核细胞中的表达及临床意义。方法选取脓毒症患儿87例,根据患儿预后情况分为死亡组（25例）和非死亡组（62例）,以体检健康儿童50名作为对照组,采用实时荧光定量聚合酶链反应（PCR）检测脓毒症患儿确诊后12 h内和对照组体检当日外周血单个核细胞中miR-132和HMGB1 mRNA表达,利用受试者工作特征（ROC）曲线分析miR-132和HMGB1 mRNA相对表达水平对患儿预后的影响。结果脓毒症患儿外周血单个核细胞中miR-132和HMGB1 mRNA相对表达量均高于对照组（P<0.05）;死亡组患儿外周血单个核细胞中miR-132和HMGB1 mRNA相对表达量均高于非死亡组（P<0.05）;Pearson相关分析显示,脓毒症患儿外周血单个核细胞中miR-132相对表达量与HMGB1 mRNA相对表达量呈正相关（r=0.521,P<0.001）;ROC曲线分析显示,miR-132、HMGB1 mRNA相对表达量在预测患儿预后时,曲线下面积（AUC）分别为0.921 [95%可信区间（CI）0.853~0.989]、0.858（95%CI 0.769~0.947）,敏感性分别为84.0%、68.0%,特异性分别为87.1%、90.3%;二者联合检测时,AUC为0.941（95%CI 0.878~1.000）,敏感性为88.0%,特异性为91.9%。结论 miR-132和HMGB1在脓毒症患儿外周血单个核细胞中表达升高,且与患儿预后有关,可作为早期评估患儿预后的指标。

关键词: 微小RNA-132, 高迁移率族蛋白1, 脓毒症, 外周血单个核细胞, 表达

Abstract:

Objective To investigate the expression levels of microRNA-132（miR-132） and high-mobility group box-1 protein（HMGB1） in peripheral blood mononuclear cells of children with sepsis. Methods A total of 87 children with sepsis were enrolled. According to prognosis,the children were classified into death group （25 cases） and non-death group （62 cases）. A total of 50 healthy children were enrolled as control group. Sepsis group was determined within 12 h after diagnosis,and control group was determined at physical examination day. Real-time fluorescence quantitation polymerase chain reaction （PCR）was used to determine the expression levels of miR-132 and HMGB1 in peripheral blood mononuclear cells. The roles of miR-132 and HMGB1 in the prognosis of children were analyzed using receiver operating characteristic （ROC） curve. Results The relative expression levels of miR-132 and HMGB1 mRNA in peripheral blood mononuclear cells in sepsis group were higher than those in control group （P<0.05）. The relative expression levels of miR-132 and HMGB1 mRNA in peripheral blood mononuclear cells in death group were higher than those in non-death group （P<0.05）. Pearson correlation analysis showed that the relative expression level of miR-132 in peripheral blood mononuclear cells of sepsis group was positively correlated with that of HMGB1 mRNA （r=0.521,P<0.001）. ROC curve analysis showed that using the relative expression levels of miR-132 and HMGB1 mRNA in predicting the prognosis of children,the areas under ROC curves （AUC） were 0.921 [95% confidence interval（CI） 0.853-0.989] and 0.858 （95%CI 0.769-0.947）,the sensitivities were 84.0% and 68.0%,and the specificities were 87.1% and 90.3%,respectively. The AUC of the combined determination of miR-132 and HMGB1 mRNA was 0.941 （95%CI 0.878-1.000）,the sensitivity was 88.0%,and the specificity was 91.9%. Conclusions The expression levels of miR-132 and HMGB1 in peripheral blood mononuclear cells of children with sepsis are increased,and they are related to the prognosis of children. They could be used as early assessment indices for the prognosis of children.

Key words: MicroRNA-132, High-mobility group box-1 protein, Sepsis, Peripheral blood mononuclear cell, Expression

中图分类号:

R446.1

张翠. 微小RNA-132和高迁移率族蛋白1在脓毒症患儿外周血单个核细胞中的表达及临床意义[J]. 检验医学, 2018, 33(9): 815-818.

ZHANG Cui. Expression levels of microRNA-132 and high-mobility group box-1 protein in peripheral blood mononuclear cells of children with sepsis[J]. Laboratory Medicine, 2018, 33(9): 815-818.

图/表 2

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